An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.

<h4>Background</h4>The stabilization or regulated reorganization of the actin cytoskeleton is essential for cellular structure and function. Recently, we could show that the activation of the SK3-channel that represents the predominant SK-channel in neural stem cells, leads to a rapid lo...

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Autores principales:	Stefan Liebau, Julie Steinestel, Leonhard Linta, Alexander Kleger, Alexander Storch, Michael Schoen, Konrad Steinestel, Christian Proepper, Juergen Bockmann, Michael J Schmeisser, Tobias M Boeckers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e0ea8c5a24d4b38b35ba8be100c8676
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spelling	oai:doaj.org-article:0e0ea8c5a24d4b38b35ba8be100c86762021-11-18T06:56:48ZAn SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.1932-620310.1371/journal.pone.0018148https://doaj.org/article/0e0ea8c5a24d4b38b35ba8be100c86762011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21464958/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The stabilization or regulated reorganization of the actin cytoskeleton is essential for cellular structure and function. Recently, we could show that the activation of the SK3-channel that represents the predominant SK-channel in neural stem cells, leads to a rapid local outgrowth of long filopodial processes. This observation indicates that the rearrangement of the actin based cytoskeleton via membrane bound SK3-channels might selectively be controlled in defined micro compartments of the cell.<h4>Principal findings</h4>We found two important proteins for cytoskeletal rearrangement, the Abelson interacting protein 1, Abi-1 and the neural Wiskott Aldrich Syndrome Protein, nWASP, to be in complex with SK3- channels in neural stem cells (NSCs). Moreover, this interaction is also found in spines and postsynaptic compartments of developing primary hippocampal neurons and regulates neurite outgrowth during early phases of differentiation. Overexpression of the proteins or pharmacological activation of SK3 channels induces obvious structural changes in NSCs and hippocampal neurons. In both neuronal cell systems SK3 channels and nWASP act synergistic by strongly inducing filopodial outgrowth while Abi-1 behaves antagonistic to its interaction partners.<h4>Conclusions</h4>Our results give good evidence for a functional interplay of a trimeric complex that transforms incoming signals via SK3-channel activation into the local rearrangement of the cytoskeleton in early steps of neuronal differentiation involving nWASP and Abi-1 actin binding proteins.Stefan LiebauJulie SteinestelLeonhard LintaAlexander KlegerAlexander StorchMichael SchoenKonrad SteinestelChristian ProepperJuergen BockmannMichael J SchmeisserTobias M BoeckersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e18148 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Liebau Julie Steinestel Leonhard Linta Alexander Kleger Alexander Storch Michael Schoen Konrad Steinestel Christian Proepper Juergen Bockmann Michael J Schmeisser Tobias M Boeckers An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
description	<h4>Background</h4>The stabilization or regulated reorganization of the actin cytoskeleton is essential for cellular structure and function. Recently, we could show that the activation of the SK3-channel that represents the predominant SK-channel in neural stem cells, leads to a rapid local outgrowth of long filopodial processes. This observation indicates that the rearrangement of the actin based cytoskeleton via membrane bound SK3-channels might selectively be controlled in defined micro compartments of the cell.<h4>Principal findings</h4>We found two important proteins for cytoskeletal rearrangement, the Abelson interacting protein 1, Abi-1 and the neural Wiskott Aldrich Syndrome Protein, nWASP, to be in complex with SK3- channels in neural stem cells (NSCs). Moreover, this interaction is also found in spines and postsynaptic compartments of developing primary hippocampal neurons and regulates neurite outgrowth during early phases of differentiation. Overexpression of the proteins or pharmacological activation of SK3 channels induces obvious structural changes in NSCs and hippocampal neurons. In both neuronal cell systems SK3 channels and nWASP act synergistic by strongly inducing filopodial outgrowth while Abi-1 behaves antagonistic to its interaction partners.<h4>Conclusions</h4>Our results give good evidence for a functional interplay of a trimeric complex that transforms incoming signals via SK3-channel activation into the local rearrangement of the cytoskeleton in early steps of neuronal differentiation involving nWASP and Abi-1 actin binding proteins.
format	article
author	Stefan Liebau Julie Steinestel Leonhard Linta Alexander Kleger Alexander Storch Michael Schoen Konrad Steinestel Christian Proepper Juergen Bockmann Michael J Schmeisser Tobias M Boeckers
author_facet	Stefan Liebau Julie Steinestel Leonhard Linta Alexander Kleger Alexander Storch Michael Schoen Konrad Steinestel Christian Proepper Juergen Bockmann Michael J Schmeisser Tobias M Boeckers
author_sort	Stefan Liebau
title	An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
title_short	An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
title_full	An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
title_fullStr	An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
title_full_unstemmed	An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
title_sort	sk3 channel/nwasp/abi-1 complex is involved in early neurogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/0e0ea8c5a24d4b38b35ba8be100c8676
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An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.

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