Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis

Abstract Vitrification is a well-accepted procedure for cryopreservation of gametes and embryos. Less is known, however, about its performance in preserving ovarian tissue, for which slow freezing is the current convention. Increasing interest is being focused on vitrification, but there are as yet...

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Autores principales:	Qingquan Shi, Yidong Xie, Yan Wang, Shangwei Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e10ba33464547e592cb1295710bc6d6
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spelling	oai:doaj.org-article:0e10ba33464547e592cb1295710bc6d62021-12-02T11:52:44ZVitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis10.1038/s41598-017-09005-72045-2322https://doaj.org/article/0e10ba33464547e592cb1295710bc6d62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09005-7https://doaj.org/toc/2045-2322Abstract Vitrification is a well-accepted procedure for cryopreservation of gametes and embryos. Less is known, however, about its performance in preserving ovarian tissue, for which slow freezing is the current convention. Increasing interest is being focused on vitrification, but there are as yet no standard protocols for its use with ovarian tissue. In part, this is because of the variety of cell types and complex nature of ovarian tissue. We performed a meta-analysis of 14 studies that compared vitrification with slow freezing for cryopreservation of ovarian tissue. In the pooled analysis, there was no significant difference between the two methods in terms of the proportion of intact primordial follicles, but vitrification was associated with significantly less DNA damage. Secondary endpoints included the number of stromal cells, significantly higher with vitrification, and primordial follicle density, which did not differ between the two methods. The present meta-analysis suggests that vitrification may be more effective than slow freezing, with less primordial follicular DNA strand breaks and better preservation of stromal cells. These advantages should lead to improved ovarian function after transplantation.Qingquan ShiYidong XieYan WangShangwei LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingquan Shi Yidong Xie Yan Wang Shangwei Li Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
description	Abstract Vitrification is a well-accepted procedure for cryopreservation of gametes and embryos. Less is known, however, about its performance in preserving ovarian tissue, for which slow freezing is the current convention. Increasing interest is being focused on vitrification, but there are as yet no standard protocols for its use with ovarian tissue. In part, this is because of the variety of cell types and complex nature of ovarian tissue. We performed a meta-analysis of 14 studies that compared vitrification with slow freezing for cryopreservation of ovarian tissue. In the pooled analysis, there was no significant difference between the two methods in terms of the proportion of intact primordial follicles, but vitrification was associated with significantly less DNA damage. Secondary endpoints included the number of stromal cells, significantly higher with vitrification, and primordial follicle density, which did not differ between the two methods. The present meta-analysis suggests that vitrification may be more effective than slow freezing, with less primordial follicular DNA strand breaks and better preservation of stromal cells. These advantages should lead to improved ovarian function after transplantation.
format	article
author	Qingquan Shi Yidong Xie Yan Wang Shangwei Li
author_facet	Qingquan Shi Yidong Xie Yan Wang Shangwei Li
author_sort	Qingquan Shi
title	Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
title_short	Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
title_full	Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
title_fullStr	Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
title_full_unstemmed	Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
title_sort	vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0e10ba33464547e592cb1295710bc6d6
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Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis

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