Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention

Claudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of <i>Clostridium perfringens</i> enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has b...

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Autores principales:	Suhayla Alnajjar, Ingo Nolte, Annegret Becker, Jan Torben Schille, Nares Trakooljul, Marcus Frank, Anaclet Ngezahayo, Hugo Murua Escobar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell lines prostate cancer C-CPE gold nanoparticle GNOME-LP Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0e116d1d8c1843d6b0d3ee07971c7aac
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spelling	oai:doaj.org-article:0e116d1d8c1843d6b0d3ee07971c7aac2021-11-25T17:55:03ZAblation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention10.3390/ijms2222122891422-00671661-6596https://doaj.org/article/0e116d1d8c1843d6b0d3ee07971c7aac2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12289https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Claudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of <i>Clostridium perfringens</i> enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for prostate adenocarcinoma (PAC) and transitional cell carcinoma (TCC) cell killing in vitro using gold-nanoparticle-mediated laser perforation (GNOME-LP). A PAC and TCC cell lines, as well as red fluorescence variants, allowing deep tissue imaging, were used. CLDN-3, -4, and -7 expression was confirmed by qPCR and immunofluorescences. The binding of C-CPE-AuNPs complexes on the cell surface was examined by scanning electron microscopy (SEM). Further, transcriptome analysis was carried out to evaluate the effect of C-CPE binder on the biological response of treated cells. Directed C-CPE-AuNP binding verified the capability to target CLDN receptors. Transcriptome analysis showed that C-CPE binding may activate immune and inflammatory responses but does not directly affect cell survival. Cancer cells ablation was demonstrated using a combination of GNOME-LP and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10% depending on cell line. The fluorescent cell lines and the verified proof of concept in vitro provide the basis for perspective xenograft studies in an animal model.Suhayla AlnajjarIngo NolteAnnegret BeckerJan Torben SchilleNares TrakooljulMarcus FrankAnaclet NgezahayoHugo Murua EscobarMDPI AGarticlecell linesprostate cancerC-CPEgold nanoparticleGNOME-LPBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12289, p 12289 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell lines prostate cancer C-CPE gold nanoparticle GNOME-LP Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cell lines prostate cancer C-CPE gold nanoparticle GNOME-LP Biology (General) QH301-705.5 Chemistry QD1-999 Suhayla Alnajjar Ingo Nolte Annegret Becker Jan Torben Schille Nares Trakooljul Marcus Frank Anaclet Ngezahayo Hugo Murua Escobar Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
description	Claudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of <i>Clostridium perfringens</i> enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for prostate adenocarcinoma (PAC) and transitional cell carcinoma (TCC) cell killing in vitro using gold-nanoparticle-mediated laser perforation (GNOME-LP). A PAC and TCC cell lines, as well as red fluorescence variants, allowing deep tissue imaging, were used. CLDN-3, -4, and -7 expression was confirmed by qPCR and immunofluorescences. The binding of C-CPE-AuNPs complexes on the cell surface was examined by scanning electron microscopy (SEM). Further, transcriptome analysis was carried out to evaluate the effect of C-CPE binder on the biological response of treated cells. Directed C-CPE-AuNP binding verified the capability to target CLDN receptors. Transcriptome analysis showed that C-CPE binding may activate immune and inflammatory responses but does not directly affect cell survival. Cancer cells ablation was demonstrated using a combination of GNOME-LP and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10% depending on cell line. The fluorescent cell lines and the verified proof of concept in vitro provide the basis for perspective xenograft studies in an animal model.
format	article
author	Suhayla Alnajjar Ingo Nolte Annegret Becker Jan Torben Schille Nares Trakooljul Marcus Frank Anaclet Ngezahayo Hugo Murua Escobar
author_facet	Suhayla Alnajjar Ingo Nolte Annegret Becker Jan Torben Schille Nares Trakooljul Marcus Frank Anaclet Ngezahayo Hugo Murua Escobar
author_sort	Suhayla Alnajjar
title	Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
title_short	Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
title_full	Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
title_fullStr	Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
title_full_unstemmed	Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
title_sort	ablation of red stable transfected claudin expressing canine prostate adenocarcinoma and transitional cell carcinoma cell lines by c-cpe gold-nanoparticle-mediated laser intervention
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0e116d1d8c1843d6b0d3ee07971c7aac
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Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention

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