Targeting chromosome trisomy for chromosome editing

Abstract A trisomy is a type of aneuploidy characterised by an additional chromosome. The additional chromosome theoretically accepts any kind of changes since it is not necessary for cellular proliferation. This advantage led us to apply two chromosome manipulation methods to autosomal trisomy in c...

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Autores principales:	Takuya Abe, Yuya Suzuki, Teppei Ikeya, Kouji Hirota
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e11f84f775b4b3cbe945e698523f711
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spelling	oai:doaj.org-article:0e11f84f775b4b3cbe945e698523f7112021-12-02T17:41:18ZTargeting chromosome trisomy for chromosome editing10.1038/s41598-021-97580-12045-2322https://doaj.org/article/0e11f84f775b4b3cbe945e698523f7112021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97580-1https://doaj.org/toc/2045-2322Abstract A trisomy is a type of aneuploidy characterised by an additional chromosome. The additional chromosome theoretically accepts any kind of changes since it is not necessary for cellular proliferation. This advantage led us to apply two chromosome manipulation methods to autosomal trisomy in chicken DT40 cells. We first corrected chromosome 2 trisomy to disomy by employing counter-selection markers. Upon construction of cells carrying markers targeted in one of the trisomic chromosome 2s, cells that have lost markers integrated in chromosome 2 were subsequently selected. The loss of one of the chromosome 2s had little impacts on the proliferative capacity, indicating unsubstantial role of the additional chromosome 2 in DT40 cells. We next tested large-scale truncations of chromosome 2 to make a mini-chromosome for the assessment of chromosome stability by introducing telomere repeat sequences to delete most of p-arm or q-arm of chromosome 2. The obtained cell lines had 0.7 Mb mini-chromosome, and approximately 0.2% of mini-chromosome was lost per cell division in wild-type background while the rate of chromosome loss was significantly increased by the depletion of DDX11, a cohesin regulatory protein. Collectively, our findings propose that trisomic chromosomes are good targets to make unique artificial chromosomes.Takuya AbeYuya SuzukiTeppei IkeyaKouji HirotaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takuya Abe Yuya Suzuki Teppei Ikeya Kouji Hirota Targeting chromosome trisomy for chromosome editing
description	Abstract A trisomy is a type of aneuploidy characterised by an additional chromosome. The additional chromosome theoretically accepts any kind of changes since it is not necessary for cellular proliferation. This advantage led us to apply two chromosome manipulation methods to autosomal trisomy in chicken DT40 cells. We first corrected chromosome 2 trisomy to disomy by employing counter-selection markers. Upon construction of cells carrying markers targeted in one of the trisomic chromosome 2s, cells that have lost markers integrated in chromosome 2 were subsequently selected. The loss of one of the chromosome 2s had little impacts on the proliferative capacity, indicating unsubstantial role of the additional chromosome 2 in DT40 cells. We next tested large-scale truncations of chromosome 2 to make a mini-chromosome for the assessment of chromosome stability by introducing telomere repeat sequences to delete most of p-arm or q-arm of chromosome 2. The obtained cell lines had 0.7 Mb mini-chromosome, and approximately 0.2% of mini-chromosome was lost per cell division in wild-type background while the rate of chromosome loss was significantly increased by the depletion of DDX11, a cohesin regulatory protein. Collectively, our findings propose that trisomic chromosomes are good targets to make unique artificial chromosomes.
format	article
author	Takuya Abe Yuya Suzuki Teppei Ikeya Kouji Hirota
author_facet	Takuya Abe Yuya Suzuki Teppei Ikeya Kouji Hirota
author_sort	Takuya Abe
title	Targeting chromosome trisomy for chromosome editing
title_short	Targeting chromosome trisomy for chromosome editing
title_full	Targeting chromosome trisomy for chromosome editing
title_fullStr	Targeting chromosome trisomy for chromosome editing
title_full_unstemmed	Targeting chromosome trisomy for chromosome editing
title_sort	targeting chromosome trisomy for chromosome editing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0e11f84f775b4b3cbe945e698523f711
work_keys_str_mv	AT takuyaabe targetingchromosometrisomyforchromosomeediting AT yuyasuzuki targetingchromosometrisomyforchromosomeediting AT teppeiikeya targetingchromosometrisomyforchromosomeediting AT koujihirota targetingchromosometrisomyforchromosomeediting
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Targeting chromosome trisomy for chromosome editing

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