Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt

Although food is very important for human life, it may be life-threatening. Foodborne diseases are spreading worldwide through the increasing rate of fresh and undercooked food consumption. Foodborne pathogens include many types of bacterial species. This study was conducted to determine the prevale...

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Autores principales:	Aya A.A. Ahmed, Heba N. Deif, Aalaa Saad, Kamelia M. Osman
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	acinetobacter ast meat pcr virulence Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://doaj.org/article/0e1e223842e34beb8f5f207337847000
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spelling	oai:doaj.org-article:0e1e223842e34beb8f5f2073378470002021-12-02T19:19:02ZMicrobiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt10.21608/javs.2021.91450.11011687-40722090-3308https://doaj.org/article/0e1e223842e34beb8f5f2073378470002021-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_196675.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Although food is very important for human life, it may be life-threatening. Foodborne diseases are spreading worldwide through the increasing rate of fresh and undercooked food consumption. Foodborne pathogens include many types of bacterial species. This study was conducted to determine the prevalence of Acinetobacter species isolated from meat samples and their phenotypic characteristics, antimicrobial resistance profiles, and genotypic characteristics. A total of 110 samples, collected from chickens (n=50), beef (n=44), rabbits (n=10), and mutton (n=6), were examined bacteriologically. The suspected colonies were identified biochemically and then tested for antimicrobial resistance, biofilm formation, and hemolytic activity; identification was confirmed by Polymerase Chain Reaction (PCR) for the genes; rpoB, tarT, fimH, and espA. Nine Acinetobacter species isolates (8.2%) were recovered. Fifty five samples resulted in the isolation of non-lactose fermenters with an incidence of 50%, 29 produced late lactose fermenters with an incidence of 26%. The rest of the samples showed no growth or non-lactose fermenters. On antibiogram, the isolates showed high resistance to ceftriaxone, imipenem, ceftazidime and ticarcillin/clavulanic acid in percentages of 89%, 77.8%, 66.7% and 66.7 %, respectively. While low resistance was found to sulfamethazole/ trimethoprim, doxycycline and amikacin in percentages of 44.4%, 33.3% and 11.1%, respectively. However, the isolates showed no resistance to ciprofloxacin. All the isolates were MDR with MDRindex (more than 0.5). Only one isolate was a weak biofilm producer but, no isolates produced hemolysis of the sheep RBCs. Genetically, 88.9% of the isolates expressed tarT and fimH genes, while only 5.6% of the isolates expressed espA gene. It can be concluded that Acinetobacter species are to be considered when inspecting meat samples of different sources.Aya A.A. AhmedHeba N. Deif Aalaa SaadKamelia M. OsmanEgyptian Society for Animal ManagementarticleacinetobacterastmeatpcrvirulenceZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 4, Pp 67-74 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acinetobacter ast meat pcr virulence Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	acinetobacter ast meat pcr virulence Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Aya A.A. Ahmed Heba N. Deif Aalaa Saad Kamelia M. Osman Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt
description	Although food is very important for human life, it may be life-threatening. Foodborne diseases are spreading worldwide through the increasing rate of fresh and undercooked food consumption. Foodborne pathogens include many types of bacterial species. This study was conducted to determine the prevalence of Acinetobacter species isolated from meat samples and their phenotypic characteristics, antimicrobial resistance profiles, and genotypic characteristics. A total of 110 samples, collected from chickens (n=50), beef (n=44), rabbits (n=10), and mutton (n=6), were examined bacteriologically. The suspected colonies were identified biochemically and then tested for antimicrobial resistance, biofilm formation, and hemolytic activity; identification was confirmed by Polymerase Chain Reaction (PCR) for the genes; rpoB, tarT, fimH, and espA. Nine Acinetobacter species isolates (8.2%) were recovered. Fifty five samples resulted in the isolation of non-lactose fermenters with an incidence of 50%, 29 produced late lactose fermenters with an incidence of 26%. The rest of the samples showed no growth or non-lactose fermenters. On antibiogram, the isolates showed high resistance to ceftriaxone, imipenem, ceftazidime and ticarcillin/clavulanic acid in percentages of 89%, 77.8%, 66.7% and 66.7 %, respectively. While low resistance was found to sulfamethazole/ trimethoprim, doxycycline and amikacin in percentages of 44.4%, 33.3% and 11.1%, respectively. However, the isolates showed no resistance to ciprofloxacin. All the isolates were MDR with MDRindex (more than 0.5). Only one isolate was a weak biofilm producer but, no isolates produced hemolysis of the sheep RBCs. Genetically, 88.9% of the isolates expressed tarT and fimH genes, while only 5.6% of the isolates expressed espA gene. It can be concluded that Acinetobacter species are to be considered when inspecting meat samples of different sources.
format	article
author	Aya A.A. Ahmed Heba N. Deif Aalaa Saad Kamelia M. Osman
author_facet	Aya A.A. Ahmed Heba N. Deif Aalaa Saad Kamelia M. Osman
author_sort	Aya A.A. Ahmed
title	Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt
title_short	Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt
title_full	Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt
title_fullStr	Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt
title_full_unstemmed	Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt
title_sort	microbiological, phenotypic characteristics, antimicrobial resistance profile and molecular identification of acinetobacter species isolated from meat of different sources in egypt
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://doaj.org/article/0e1e223842e34beb8f5f207337847000
work_keys_str_mv	AT ayaaaahmed microbiologicalphenotypiccharacteristicsantimicrobialresistanceprofileandmolecularidentificationofacinetobacterspeciesisolatedfrommeatofdifferentsourcesinegypt AT hebandeif microbiologicalphenotypiccharacteristicsantimicrobialresistanceprofileandmolecularidentificationofacinetobacterspeciesisolatedfrommeatofdifferentsourcesinegypt AT aalaasaad microbiologicalphenotypiccharacteristicsantimicrobialresistanceprofileandmolecularidentificationofacinetobacterspeciesisolatedfrommeatofdifferentsourcesinegypt AT kameliamosman microbiologicalphenotypiccharacteristicsantimicrobialresistanceprofileandmolecularidentificationofacinetobacterspeciesisolatedfrommeatofdifferentsourcesinegypt
_version_	1718376837121310720

Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt

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