Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure

Abstract The aim of this study was to evaluate the nature and mechanisms of interaction between HIV peptide/dendrimer complexes (dendriplex) and artificial lipid membranes, such as large unilayered vesicles (LUV) and lipid monolayers in the air–water interface. Dendriplexes were combined as one of t...

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Autores principales:	Katarzyna Milowska, Aleksandra Rodacka, Sophie Melikishvili, Adam Buczkowski, Bartlomiej Pałecz, Iveta Waczulikova, Tibor Hianik, Jean Pierre Majoral, Maksim Ionov, Maria Bryszewska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e219cc9702a4453833b0b5af19d3194
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spelling	oai:doaj.org-article:0e219cc9702a4453833b0b5af19d31942021-12-02T16:45:40ZDendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure10.1038/s41598-021-96194-x2045-2322https://doaj.org/article/0e219cc9702a4453833b0b5af19d31942021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96194-xhttps://doaj.org/toc/2045-2322Abstract The aim of this study was to evaluate the nature and mechanisms of interaction between HIV peptide/dendrimer complexes (dendriplex) and artificial lipid membranes, such as large unilayered vesicles (LUV) and lipid monolayers in the air–water interface. Dendriplexes were combined as one of three HIV-derived peptides (Gp160, P24 and Nef) and one of two cationic phosphorus dendrimers (CPD-G3 and CPD-G4). LUVs were formed of 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) or of a mixture of DMPC and dipalmitoyl-phosphatidylglycerol (DPPG). Interactions between dendriplexes and vesicles were characterized by dynamic light scattering (DLS), fluorescence anisotropy, differential scanning calorimetry (DSC) and Langmuir–Blodgett methods. The morphology of formed systems was examined by transmission electron microscopy (TEM). The results suggest that dendriplexes interact with both hydrophobic and hydrophilic regions of lipid bilayers. The interactions between dendriplexes and negatively charged lipids (DMPC–DPPG) were stronger than those between dendriplexes and liposomes composed of zwitterionic lipids (DMPC). The former were primarily of electrostatic nature due to the positive charge of dendriplexes and the negative charge of the membrane, whereas the latter can be attributed to disturbances in the hydrophobic domain of the membrane. Obtained results provide new information about mechanisms of interaction between lipid membranes and nanocomplexes formed with HIV-derived peptides and phosphorus dendrimers. These data could be important for the choosing the appropriate antigen delivery vehicle in the new vaccines against HIV infection.Katarzyna MilowskaAleksandra RodackaSophie MelikishviliAdam BuczkowskiBartlomiej PałeczIveta WaczulikovaTibor HianikJean Pierre MajoralMaksim IonovMaria BryszewskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna Milowska Aleksandra Rodacka Sophie Melikishvili Adam Buczkowski Bartlomiej Pałecz Iveta Waczulikova Tibor Hianik Jean Pierre Majoral Maksim Ionov Maria Bryszewska Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure
description	Abstract The aim of this study was to evaluate the nature and mechanisms of interaction between HIV peptide/dendrimer complexes (dendriplex) and artificial lipid membranes, such as large unilayered vesicles (LUV) and lipid monolayers in the air–water interface. Dendriplexes were combined as one of three HIV-derived peptides (Gp160, P24 and Nef) and one of two cationic phosphorus dendrimers (CPD-G3 and CPD-G4). LUVs were formed of 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) or of a mixture of DMPC and dipalmitoyl-phosphatidylglycerol (DPPG). Interactions between dendriplexes and vesicles were characterized by dynamic light scattering (DLS), fluorescence anisotropy, differential scanning calorimetry (DSC) and Langmuir–Blodgett methods. The morphology of formed systems was examined by transmission electron microscopy (TEM). The results suggest that dendriplexes interact with both hydrophobic and hydrophilic regions of lipid bilayers. The interactions between dendriplexes and negatively charged lipids (DMPC–DPPG) were stronger than those between dendriplexes and liposomes composed of zwitterionic lipids (DMPC). The former were primarily of electrostatic nature due to the positive charge of dendriplexes and the negative charge of the membrane, whereas the latter can be attributed to disturbances in the hydrophobic domain of the membrane. Obtained results provide new information about mechanisms of interaction between lipid membranes and nanocomplexes formed with HIV-derived peptides and phosphorus dendrimers. These data could be important for the choosing the appropriate antigen delivery vehicle in the new vaccines against HIV infection.
format	article
author	Katarzyna Milowska Aleksandra Rodacka Sophie Melikishvili Adam Buczkowski Bartlomiej Pałecz Iveta Waczulikova Tibor Hianik Jean Pierre Majoral Maksim Ionov Maria Bryszewska
author_facet	Katarzyna Milowska Aleksandra Rodacka Sophie Melikishvili Adam Buczkowski Bartlomiej Pałecz Iveta Waczulikova Tibor Hianik Jean Pierre Majoral Maksim Ionov Maria Bryszewska
author_sort	Katarzyna Milowska
title	Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure
title_short	Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure
title_full	Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure
title_fullStr	Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure
title_full_unstemmed	Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure
title_sort	dendrimeric hiv-peptide delivery nanosystem affects lipid membranes structure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0e219cc9702a4453833b0b5af19d3194
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Dendrimeric HIV-peptide delivery nanosystem affects lipid membranes structure

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