Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals

The detection capacity of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) in tongues from dead animals in breeding herds (stillborns and piglets dying during the lactating period) and nursery farms (naturally dead animals) for PRRSV surveillance was evaluated. The samples were selected i...

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Autores principales:	Jordi Baliellas, Elena Novell, Vicens Enric-Tarancón, Carles Vilalta, Lorenzo Fraile
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PRRSV monitoring tongues exudate swine Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/0e2c01d175eb443eb47a1b56cba6a65d
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spelling	oai:doaj.org-article:0e2c01d175eb443eb47a1b56cba6a65d2021-11-25T19:11:56ZPorcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals10.3390/vetsci81102592306-7381https://doaj.org/article/0e2c01d175eb443eb47a1b56cba6a65d2021-11-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/259https://doaj.org/toc/2306-7381The detection capacity of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) in tongues from dead animals in breeding herds (stillborns and piglets dying during the lactating period) and nursery farms (naturally dead animals) for PRRSV surveillance was evaluated. The samples were selected if pairs of serum and tongues were available from 2018 to 2020. Serum (pools of five) and exudate from tongues (one bag) were analyzed by PRRSV RT-PCR. The agreement between the serum sample procedure versus tongues exudate was assessed using a concordance test (Kappa statistic) at batch level. A total of 32 submissions, corresponding to 14 farms, had PRRSV diagnostic information for serum and tongues exudate. The overall agreement of batch classification as positive or negative, based on RT-PCR PRRSV results, between serum and tongue exudate of the 32 pairs was 76.9%. Cohen’s Kappa was 0.55. The main discrepancy came from the presence of positive samples in tongues exudate and not in serum, suggesting that tongue exudate to monitor PRRSV seems to be more sensitive than serum. These results suggest that this sample procedure could be also used for PRRSV surveillance and monitoring.Jordi BaliellasElena NovellVicens Enric-TarancónCarles VilaltaLorenzo FraileMDPI AGarticlePRRSVmonitoringtongues exudateswineVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 259, p 259 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PRRSV monitoring tongues exudate swine Veterinary medicine SF600-1100
spellingShingle	PRRSV monitoring tongues exudate swine Veterinary medicine SF600-1100 Jordi Baliellas Elena Novell Vicens Enric-Tarancón Carles Vilalta Lorenzo Fraile Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
description	The detection capacity of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) in tongues from dead animals in breeding herds (stillborns and piglets dying during the lactating period) and nursery farms (naturally dead animals) for PRRSV surveillance was evaluated. The samples were selected if pairs of serum and tongues were available from 2018 to 2020. Serum (pools of five) and exudate from tongues (one bag) were analyzed by PRRSV RT-PCR. The agreement between the serum sample procedure versus tongues exudate was assessed using a concordance test (Kappa statistic) at batch level. A total of 32 submissions, corresponding to 14 farms, had PRRSV diagnostic information for serum and tongues exudate. The overall agreement of batch classification as positive or negative, based on RT-PCR PRRSV results, between serum and tongue exudate of the 32 pairs was 76.9%. Cohen’s Kappa was 0.55. The main discrepancy came from the presence of positive samples in tongues exudate and not in serum, suggesting that tongue exudate to monitor PRRSV seems to be more sensitive than serum. These results suggest that this sample procedure could be also used for PRRSV surveillance and monitoring.
format	article
author	Jordi Baliellas Elena Novell Vicens Enric-Tarancón Carles Vilalta Lorenzo Fraile
author_facet	Jordi Baliellas Elena Novell Vicens Enric-Tarancón Carles Vilalta Lorenzo Fraile
author_sort	Jordi Baliellas
title	Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
title_short	Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
title_full	Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
title_fullStr	Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
title_full_unstemmed	Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
title_sort	porcine reproductive and respiratory syndrome surveillance in breeding herds and nurseries using tongue tips from dead animals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0e2c01d175eb443eb47a1b56cba6a65d
work_keys_str_mv	AT jordibaliellas porcinereproductiveandrespiratorysyndromesurveillanceinbreedingherdsandnurseriesusingtonguetipsfromdeadanimals AT elenanovell porcinereproductiveandrespiratorysyndromesurveillanceinbreedingherdsandnurseriesusingtonguetipsfromdeadanimals AT vicensenrictarancon porcinereproductiveandrespiratorysyndromesurveillanceinbreedingherdsandnurseriesusingtonguetipsfromdeadanimals AT carlesvilalta porcinereproductiveandrespiratorysyndromesurveillanceinbreedingherdsandnurseriesusingtonguetipsfromdeadanimals AT lorenzofraile porcinereproductiveandrespiratorysyndromesurveillanceinbreedingherdsandnurseriesusingtonguetipsfromdeadanimals
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Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals

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