Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning

Abstract Extracellular vesicles (EVs) such as exosomes are nano-sized vesicles that carry proteins and miRNAs and can transmit signals between cells. We hypothesized that exosomes from endothelial cells can transmit protective signals to cardiomyocytes. Co-culture of primary adult rat cardiomyocytes...

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Autores principales:	Sean M. Davidson, Jaime A. Riquelme, Ying Zheng, Jose M. Vicencio, Sergio Lavandero, Derek M. Yellon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Ischaemic Preconditioning (IPC) Human Umbilical Vein Endothelial Cells (HUVEC) Nanoparticle Tracking Analysis (NTA) Exosome Release HUVEC Cells Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e2d4a96a5c74a57b33fabee88b3c25c
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spelling	oai:doaj.org-article:0e2d4a96a5c74a57b33fabee88b3c25c2021-12-02T11:40:46ZEndothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning10.1038/s41598-018-34357-z2045-2322https://doaj.org/article/0e2d4a96a5c74a57b33fabee88b3c25c2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34357-zhttps://doaj.org/toc/2045-2322Abstract Extracellular vesicles (EVs) such as exosomes are nano-sized vesicles that carry proteins and miRNAs and can transmit signals between cells. We hypothesized that exosomes from endothelial cells can transmit protective signals to cardiomyocytes. Co-culture of primary adult rat cardiomyocytes with normoxic HUVEC cells separated by a cell-impermeable membrane reduced the percentage of cardiomyocyte death following simulated ischaemia and reperfusion (sIR) from 80 ± 11% to 51 ± 4% (P < 0.05; N = 5). When EVs were removed from the HUVEC-conditioned medium it was no longer protective. Exosomes were purified from HUVEC-conditioned medium using differential centrifugation and characterized by nanoparticle tracking analysis, electron microscopy, and flow cytometry. Pre-incubation of cardiomyocytes with HUVEC exosomes reduced the percentage of cell death after sIR from 88 ± 4% to 55 ± 3% (P < 0.05; N = 3). This protection required ERK1/2 activity as it was prevented by inhibitors PD98059 and U0126. Ischaemic preconditioning caused about ~3-fold higher rate of exosome production from HUVEC and from isolated, perfused rat hearts. This increase resulted in significantly greater protection against sIR in cardiomyocytes. In conclusion, exosomes released from endothelial cells can confer resistance to sIR injury in cardiomyocytes via the activation of the ERK1/2 MAPK signalling pathway, and may contribute to IPC.Sean M. DavidsonJaime A. RiquelmeYing ZhengJose M. VicencioSergio LavanderoDerek M. YellonNature PortfolioarticleIschaemic Preconditioning (IPC)Human Umbilical Vein Endothelial Cells (HUVEC)Nanoparticle Tracking Analysis (NTA)Exosome ReleaseHUVEC CellsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ischaemic Preconditioning (IPC) Human Umbilical Vein Endothelial Cells (HUVEC) Nanoparticle Tracking Analysis (NTA) Exosome Release HUVEC Cells Medicine R Science Q
spellingShingle	Ischaemic Preconditioning (IPC) Human Umbilical Vein Endothelial Cells (HUVEC) Nanoparticle Tracking Analysis (NTA) Exosome Release HUVEC Cells Medicine R Science Q Sean M. Davidson Jaime A. Riquelme Ying Zheng Jose M. Vicencio Sergio Lavandero Derek M. Yellon Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
description	Abstract Extracellular vesicles (EVs) such as exosomes are nano-sized vesicles that carry proteins and miRNAs and can transmit signals between cells. We hypothesized that exosomes from endothelial cells can transmit protective signals to cardiomyocytes. Co-culture of primary adult rat cardiomyocytes with normoxic HUVEC cells separated by a cell-impermeable membrane reduced the percentage of cardiomyocyte death following simulated ischaemia and reperfusion (sIR) from 80 ± 11% to 51 ± 4% (P < 0.05; N = 5). When EVs were removed from the HUVEC-conditioned medium it was no longer protective. Exosomes were purified from HUVEC-conditioned medium using differential centrifugation and characterized by nanoparticle tracking analysis, electron microscopy, and flow cytometry. Pre-incubation of cardiomyocytes with HUVEC exosomes reduced the percentage of cell death after sIR from 88 ± 4% to 55 ± 3% (P < 0.05; N = 3). This protection required ERK1/2 activity as it was prevented by inhibitors PD98059 and U0126. Ischaemic preconditioning caused about ~3-fold higher rate of exosome production from HUVEC and from isolated, perfused rat hearts. This increase resulted in significantly greater protection against sIR in cardiomyocytes. In conclusion, exosomes released from endothelial cells can confer resistance to sIR injury in cardiomyocytes via the activation of the ERK1/2 MAPK signalling pathway, and may contribute to IPC.
format	article
author	Sean M. Davidson Jaime A. Riquelme Ying Zheng Jose M. Vicencio Sergio Lavandero Derek M. Yellon
author_facet	Sean M. Davidson Jaime A. Riquelme Ying Zheng Jose M. Vicencio Sergio Lavandero Derek M. Yellon
author_sort	Sean M. Davidson
title	Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
title_short	Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
title_full	Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
title_fullStr	Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
title_full_unstemmed	Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
title_sort	endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0e2d4a96a5c74a57b33fabee88b3c25c
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Endothelial cells release cardioprotective exosomes that may contribute to ischaemic preconditioning

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