Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model

Abstract Mycobacterium avium subsp. paratuberculosis (Map) causes paratuberculosis (PTB), a granulomatous enteritis in ruminants that exerts high economic impact on the dairy industry worldwide. Current vaccines have shown to be cost-effective against Map and in some cases confer beneficial non-spec...

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Autores principales:	Iraia Ladero-Auñon, Elena Molina, Maddi Oyanguren, Diego Barriales, Miguel Fuertes, Iker A. Sevilla, Lucy Luo, Rakel Arrazuria, Jeroen De Buck, Juan Anguita, Natalia Elguezabal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/0e308e07961c4a49aaad38bedf5df7ee
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spelling	oai:doaj.org-article:0e308e07961c4a49aaad38bedf5df7ee2021-12-02T17:08:20ZOral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model10.1038/s41541-021-00367-82059-0105https://doaj.org/article/0e308e07961c4a49aaad38bedf5df7ee2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00367-8https://doaj.org/toc/2059-0105Abstract Mycobacterium avium subsp. paratuberculosis (Map) causes paratuberculosis (PTB), a granulomatous enteritis in ruminants that exerts high economic impact on the dairy industry worldwide. Current vaccines have shown to be cost-effective against Map and in some cases confer beneficial non-specific effects against other pathogens suggesting the existence of trained immunity. Although Map infection is mainly transmitted by the fecal-oral route, oral vaccination has not been deeply studied. Therefore, the aim of this study was to compare the oral route with a set of mycobacterial and non-mycobacterial vaccines with a subcutaneously administered commercially available vaccine. Training effects on polymorphonuclear neutrophils (PMNs) and homologous and heterologous in vivo protection against Map were investigated in the rabbit infection model. Oral vaccination with inactivated or live vaccines was able to activate mucosal immunity as seen by elevation of serum IgA and the expression of IL4 in peripheral blood mononuclear cells (PBMCs). In addition, peripheral PMN phagocytosis against Map was enhanced by vaccination and extracellular trap release against Map and non-related pathogens was modified by both, vaccination and Map-challenge, indicating trained immunity. Finally, PBMCs from vaccinated animals stimulated in vitro with Map antigens showed a rapid innate activation cytokine profile. In conclusion, our data show that oral vaccination against PTB can stimulate neutrophil activity and both innate and adaptive immune responses that correlate with protection.Iraia Ladero-AuñonElena MolinaMaddi OyangurenDiego BarrialesMiguel FuertesIker A. SevillaLucy LuoRakel ArrazuriaJeroen De BuckJuan AnguitaNatalia ElguezabalNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Iraia Ladero-Auñon Elena Molina Maddi Oyanguren Diego Barriales Miguel Fuertes Iker A. Sevilla Lucy Luo Rakel Arrazuria Jeroen De Buck Juan Anguita Natalia Elguezabal Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model
description	Abstract Mycobacterium avium subsp. paratuberculosis (Map) causes paratuberculosis (PTB), a granulomatous enteritis in ruminants that exerts high economic impact on the dairy industry worldwide. Current vaccines have shown to be cost-effective against Map and in some cases confer beneficial non-specific effects against other pathogens suggesting the existence of trained immunity. Although Map infection is mainly transmitted by the fecal-oral route, oral vaccination has not been deeply studied. Therefore, the aim of this study was to compare the oral route with a set of mycobacterial and non-mycobacterial vaccines with a subcutaneously administered commercially available vaccine. Training effects on polymorphonuclear neutrophils (PMNs) and homologous and heterologous in vivo protection against Map were investigated in the rabbit infection model. Oral vaccination with inactivated or live vaccines was able to activate mucosal immunity as seen by elevation of serum IgA and the expression of IL4 in peripheral blood mononuclear cells (PBMCs). In addition, peripheral PMN phagocytosis against Map was enhanced by vaccination and extracellular trap release against Map and non-related pathogens was modified by both, vaccination and Map-challenge, indicating trained immunity. Finally, PBMCs from vaccinated animals stimulated in vitro with Map antigens showed a rapid innate activation cytokine profile. In conclusion, our data show that oral vaccination against PTB can stimulate neutrophil activity and both innate and adaptive immune responses that correlate with protection.
format	article
author	Iraia Ladero-Auñon Elena Molina Maddi Oyanguren Diego Barriales Miguel Fuertes Iker A. Sevilla Lucy Luo Rakel Arrazuria Jeroen De Buck Juan Anguita Natalia Elguezabal
author_facet	Iraia Ladero-Auñon Elena Molina Maddi Oyanguren Diego Barriales Miguel Fuertes Iker A. Sevilla Lucy Luo Rakel Arrazuria Jeroen De Buck Juan Anguita Natalia Elguezabal
author_sort	Iraia Ladero-Auñon
title	Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model
title_short	Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model
title_full	Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model
title_fullStr	Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model
title_full_unstemmed	Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model
title_sort	oral vaccination stimulates neutrophil functionality and exerts protection in a mycobacterium avium subsp. paratuberculosis infection model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0e308e07961c4a49aaad38bedf5df7ee
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Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model

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