Control of directed cell migration in vivo by membrane-to-cortex attachment.

Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to...

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Autores principales:	Alba Diz-Muñoz, Michael Krieg, Martin Bergert, Itziar Ibarlucea-Benitez, Daniel J Muller, Ewa Paluch, Carl-Philipp Heisenberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0e46b57003fb4fc48fe3efd316d3fd08
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spelling	oai:doaj.org-article:0e46b57003fb4fc48fe3efd316d3fd082021-11-18T05:36:26ZControl of directed cell migration in vivo by membrane-to-cortex attachment.1544-91731545-788510.1371/journal.pbio.1000544https://doaj.org/article/0e46b57003fb4fc48fe3efd316d3fd082010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21151339/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.Alba Diz-MuñozMichael KriegMartin BergertItziar Ibarlucea-BenitezDaniel J MullerEwa PaluchCarl-Philipp HeisenbergPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 11, p e1000544 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Alba Diz-Muñoz Michael Krieg Martin Bergert Itziar Ibarlucea-Benitez Daniel J Muller Ewa Paluch Carl-Philipp Heisenberg Control of directed cell migration in vivo by membrane-to-cortex attachment.
description	Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.
format	article
author	Alba Diz-Muñoz Michael Krieg Martin Bergert Itziar Ibarlucea-Benitez Daniel J Muller Ewa Paluch Carl-Philipp Heisenberg
author_facet	Alba Diz-Muñoz Michael Krieg Martin Bergert Itziar Ibarlucea-Benitez Daniel J Muller Ewa Paluch Carl-Philipp Heisenberg
author_sort	Alba Diz-Muñoz
title	Control of directed cell migration in vivo by membrane-to-cortex attachment.
title_short	Control of directed cell migration in vivo by membrane-to-cortex attachment.
title_full	Control of directed cell migration in vivo by membrane-to-cortex attachment.
title_fullStr	Control of directed cell migration in vivo by membrane-to-cortex attachment.
title_full_unstemmed	Control of directed cell migration in vivo by membrane-to-cortex attachment.
title_sort	control of directed cell migration in vivo by membrane-to-cortex attachment.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/0e46b57003fb4fc48fe3efd316d3fd08
work_keys_str_mv	AT albadizmunoz controlofdirectedcellmigrationinvivobymembranetocortexattachment AT michaelkrieg controlofdirectedcellmigrationinvivobymembranetocortexattachment AT martinbergert controlofdirectedcellmigrationinvivobymembranetocortexattachment AT itziaribarluceabenitez controlofdirectedcellmigrationinvivobymembranetocortexattachment AT danieljmuller controlofdirectedcellmigrationinvivobymembranetocortexattachment AT ewapaluch controlofdirectedcellmigrationinvivobymembranetocortexattachment AT carlphilippheisenberg controlofdirectedcellmigrationinvivobymembranetocortexattachment
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Control of directed cell migration in vivo by membrane-to-cortex attachment.

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