Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta

Salwa Ibrahim,1,* Adam P Strange,2,* Sebastian Aguayo,2,3 Albatool Shinawi,1 Nabilah Harith,1 Nurjehan Mohamed-Ibrahim,1 Samera Siddiqui,2 Susan Parekh,1 Laurent Bozec4 1Department of Paediatric Dentistry, UCL Eastman Dental Institute, University College London, London, UK; 2Department of Biomateria...

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Autores principales:	Ibrahim S, Strange AP, Aguayo S, Shinawi A, Harith N, Mohamed-Ibrahim N, Siddiqui S, Parekh S, Bozec L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	dentin collagen dentinogenesis imperfecta demineralisation dentistry atomic force microscopy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0e4daf24d7da419f8d6a6eb67dd4493c
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id	oai:doaj.org-article:0e4daf24d7da419f8d6a6eb67dd4493c
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	dentin collagen dentinogenesis imperfecta demineralisation dentistry atomic force microscopy Medicine (General) R5-920
spellingShingle	dentin collagen dentinogenesis imperfecta demineralisation dentistry atomic force microscopy Medicine (General) R5-920 Ibrahim S Strange AP Aguayo S Shinawi A Harith N Mohamed-Ibrahim N Siddiqui S Parekh S Bozec L Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
description	Salwa Ibrahim,1,* Adam P Strange,2,* Sebastian Aguayo,2,3 Albatool Shinawi,1 Nabilah Harith,1 Nurjehan Mohamed-Ibrahim,1 Samera Siddiqui,2 Susan Parekh,1 Laurent Bozec4 1Department of Paediatric Dentistry, UCL Eastman Dental Institute, University College London, London, UK; 2Department of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, UK; 3School of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile; 4Faculty of Dentistry, University of Toronto, Toronto, Canada*These authors contributed equally to this workCorrespondence: Laurent BozecFaculty of Dentistry, University of Toronto, 124 Edward Street, Toronto ON M5G 1G6, CanadaEmail L.bozec@utoronto.caIntroduction: Dentinogenesis imperfecta type 1 (OIDI) is considered a relatively rare genetic disorder (1:5000 to 1:45,000) associated with osteogenesis imperfecta. OIDI impacts the formation of collagen fibrils in dentin, leading to morphological and structural changes that affect the strength and appearance of teeth. However, there is still a lack of understanding regarding the nanoscale characterization of the disease, in terms of collagen ultrastructure and mechanical properties. Therefore, this research presents a qualitative and quantitative report into the phenotype and characterization of OIDI in dentin, by using a combination of imaging, nanomechanical approaches.Methods: For this study, 8 primary molars from OIDI patients and 8 primary control molars were collected, embedded in acrylic resin and cut into longitudinal sections. Sections were then demineralized in 37% phosphoric acid using a protocol developed in-house. Initial experiments demonstrated the effectiveness of the demineralization protocol, as the ATR-FTIR spectral fingerprints showed an increase in the amide bands together with a decrease in phosphate content. Structural and mechanical analyses were performed directly on both the mineralized and demineralized samples using a combination of scanning electron microscopy, atomic force microscopy, and Wallace indentation.Results: Mesoscale imaging showed alterations in dentinal tubule morphology in OIDI patients, with a reduced number of tubules and a decreased tubule diameter compared to healthy controls. Nanoscale collagen ultrastructure presented a similar D-banding periodicity between OIDI and controls. Reduced collagen fibrils diameter was also recorded for the OIDI group. The hardness of the (mineralized) control dentin was found to be significantly higher (p<0.05) than that of the OIDI (mineralized) dentine. Both the exposed peri- and intratubular dentinal collagen presented bimodal elastic behaviors (Young’s moduli). The control samples presented a stiffening of the intratubular collagen when compared to the peritubular collagen. In case of the OIDI, this stiffening in the collagen between peri- and intratubular dentinal collagen was not observed and the exposed collagen presented overall a lower elasticity than the control samples.Conclusion: This study presents a systematic approach to the characterization of collagen structure and properties in OIDI as diagnosed in dentin. Structural markers for OIDI at the mesoscale and nanoscale were found and correlated with an observed lack of increased elastic moduli of the collagen fibrils in the intratubular OIDI dentin. These findings offer an explanation of how structural changes in the dentin could be responsible for the failure of some adhesive restorative materials as observed in patients affected by OIDI.Keywords: dentin, collagen, dentinogenesis imperfecta, demineralisation, dentistry, atomic force microscopy
format	article
author	Ibrahim S Strange AP Aguayo S Shinawi A Harith N Mohamed-Ibrahim N Siddiqui S Parekh S Bozec L
author_facet	Ibrahim S Strange AP Aguayo S Shinawi A Harith N Mohamed-Ibrahim N Siddiqui S Parekh S Bozec L
author_sort	Ibrahim S
title	Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
title_short	Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
title_full	Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
title_fullStr	Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
title_full_unstemmed	Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
title_sort	phenotypic properties of collagen in dentinogenesis imperfecta associated with osteogenesis imperfecta
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/0e4daf24d7da419f8d6a6eb67dd4493c
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spelling	oai:doaj.org-article:0e4daf24d7da419f8d6a6eb67dd4493c2021-12-02T06:56:54ZPhenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta1178-2013https://doaj.org/article/0e4daf24d7da419f8d6a6eb67dd4493c2019-12-01T00:00:00Zhttps://www.dovepress.com/phenotypic-properties-of-collagen-in-dentinogenesis-imperfecta-associa-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Salwa Ibrahim,1,* Adam P Strange,2,* Sebastian Aguayo,2,3 Albatool Shinawi,1 Nabilah Harith,1 Nurjehan Mohamed-Ibrahim,1 Samera Siddiqui,2 Susan Parekh,1 Laurent Bozec4 1Department of Paediatric Dentistry, UCL Eastman Dental Institute, University College London, London, UK; 2Department of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, UK; 3School of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile; 4Faculty of Dentistry, University of Toronto, Toronto, Canada*These authors contributed equally to this workCorrespondence: Laurent BozecFaculty of Dentistry, University of Toronto, 124 Edward Street, Toronto ON M5G 1G6, CanadaEmail L.bozec@utoronto.caIntroduction: Dentinogenesis imperfecta type 1 (OIDI) is considered a relatively rare genetic disorder (1:5000 to 1:45,000) associated with osteogenesis imperfecta. OIDI impacts the formation of collagen fibrils in dentin, leading to morphological and structural changes that affect the strength and appearance of teeth. However, there is still a lack of understanding regarding the nanoscale characterization of the disease, in terms of collagen ultrastructure and mechanical properties. Therefore, this research presents a qualitative and quantitative report into the phenotype and characterization of OIDI in dentin, by using a combination of imaging, nanomechanical approaches.Methods: For this study, 8 primary molars from OIDI patients and 8 primary control molars were collected, embedded in acrylic resin and cut into longitudinal sections. Sections were then demineralized in 37% phosphoric acid using a protocol developed in-house. Initial experiments demonstrated the effectiveness of the demineralization protocol, as the ATR-FTIR spectral fingerprints showed an increase in the amide bands together with a decrease in phosphate content. Structural and mechanical analyses were performed directly on both the mineralized and demineralized samples using a combination of scanning electron microscopy, atomic force microscopy, and Wallace indentation.Results: Mesoscale imaging showed alterations in dentinal tubule morphology in OIDI patients, with a reduced number of tubules and a decreased tubule diameter compared to healthy controls. Nanoscale collagen ultrastructure presented a similar D-banding periodicity between OIDI and controls. Reduced collagen fibrils diameter was also recorded for the OIDI group. The hardness of the (mineralized) control dentin was found to be significantly higher (p<0.05) than that of the OIDI (mineralized) dentine. Both the exposed peri- and intratubular dentinal collagen presented bimodal elastic behaviors (Young’s moduli). The control samples presented a stiffening of the intratubular collagen when compared to the peritubular collagen. In case of the OIDI, this stiffening in the collagen between peri- and intratubular dentinal collagen was not observed and the exposed collagen presented overall a lower elasticity than the control samples.Conclusion: This study presents a systematic approach to the characterization of collagen structure and properties in OIDI as diagnosed in dentin. Structural markers for OIDI at the mesoscale and nanoscale were found and correlated with an observed lack of increased elastic moduli of the collagen fibrils in the intratubular OIDI dentin. These findings offer an explanation of how structural changes in the dentin could be responsible for the failure of some adhesive restorative materials as observed in patients affected by OIDI.Keywords: dentin, collagen, dentinogenesis imperfecta, demineralisation, dentistry, atomic force microscopyIbrahim SStrange APAguayo SShinawi AHarith NMohamed-Ibrahim NSiddiqui SParekh SBozec LDove Medical Pressarticledentincollagendentinogenesis imperfectademineralisationdentistryatomic force microscopyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9423-9435 (2019)

Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta

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