Next-generation mammalian genetics toward organism-level systems biology

Abstract Organism-level systems biology in mammals aims to identify, analyze, control, and design molecular and cellular networks executing various biological functions in mammals. In particular, system-level identification and analysis of molecular and cellular networks can be accelerated by next-g...

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Autores principales:	Etsuo A. Susaki, Hideki Ukai, Hiroki R. Ueda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0e504e9c36d14e26a96d40ede0aa609e
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spelling	oai:doaj.org-article:0e504e9c36d14e26a96d40ede0aa609e2021-12-02T16:09:11ZNext-generation mammalian genetics toward organism-level systems biology10.1038/s41540-017-0015-22056-7189https://doaj.org/article/0e504e9c36d14e26a96d40ede0aa609e2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41540-017-0015-2https://doaj.org/toc/2056-7189Abstract Organism-level systems biology in mammals aims to identify, analyze, control, and design molecular and cellular networks executing various biological functions in mammals. In particular, system-level identification and analysis of molecular and cellular networks can be accelerated by next-generation mammalian genetics. Mammalian genetics without crossing, where all production and phenotyping studies of genome-edited animals are completed within a single generation drastically reduce the time, space, and effort of conducting the systems research. Next-generation mammalian genetics is based on recent technological advancements in genome editing and developmental engineering. The process begins with introduction of double-strand breaks into genomic DNA by using site-specific endonucleases, which results in highly efficient genome editing in mammalian zygotes or embryonic stem cells. By using nuclease-mediated genome editing in zygotes, or ~100% embryonic stem cell-derived mouse technology, whole-body knock-out and knock-in mice can be produced within a single generation. These emerging technologies allow us to produce multiple knock-out or knock-in strains in high-throughput manner. In this review, we discuss the basic concepts and related technologies as well as current challenges and future opportunities for next-generation mammalian genetics in organism-level systems biology.Etsuo A. SusakiHideki UkaiHiroki R. UedaNature PortfolioarticleBiology (General)QH301-705.5ENnpj Systems Biology and Applications, Vol 3, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Etsuo A. Susaki Hideki Ukai Hiroki R. Ueda Next-generation mammalian genetics toward organism-level systems biology
description	Abstract Organism-level systems biology in mammals aims to identify, analyze, control, and design molecular and cellular networks executing various biological functions in mammals. In particular, system-level identification and analysis of molecular and cellular networks can be accelerated by next-generation mammalian genetics. Mammalian genetics without crossing, where all production and phenotyping studies of genome-edited animals are completed within a single generation drastically reduce the time, space, and effort of conducting the systems research. Next-generation mammalian genetics is based on recent technological advancements in genome editing and developmental engineering. The process begins with introduction of double-strand breaks into genomic DNA by using site-specific endonucleases, which results in highly efficient genome editing in mammalian zygotes or embryonic stem cells. By using nuclease-mediated genome editing in zygotes, or ~100% embryonic stem cell-derived mouse technology, whole-body knock-out and knock-in mice can be produced within a single generation. These emerging technologies allow us to produce multiple knock-out or knock-in strains in high-throughput manner. In this review, we discuss the basic concepts and related technologies as well as current challenges and future opportunities for next-generation mammalian genetics in organism-level systems biology.
format	article
author	Etsuo A. Susaki Hideki Ukai Hiroki R. Ueda
author_facet	Etsuo A. Susaki Hideki Ukai Hiroki R. Ueda
author_sort	Etsuo A. Susaki
title	Next-generation mammalian genetics toward organism-level systems biology
title_short	Next-generation mammalian genetics toward organism-level systems biology
title_full	Next-generation mammalian genetics toward organism-level systems biology
title_fullStr	Next-generation mammalian genetics toward organism-level systems biology
title_full_unstemmed	Next-generation mammalian genetics toward organism-level systems biology
title_sort	next-generation mammalian genetics toward organism-level systems biology
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0e504e9c36d14e26a96d40ede0aa609e
work_keys_str_mv	AT etsuoasusaki nextgenerationmammaliangeneticstowardorganismlevelsystemsbiology AT hidekiukai nextgenerationmammaliangeneticstowardorganismlevelsystemsbiology AT hirokirueda nextgenerationmammaliangeneticstowardorganismlevelsystemsbiology
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Next-generation mammalian genetics toward organism-level systems biology

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