Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.

Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS) with a tail shock (unconditioned stimulus, UCS) expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV...

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Auteurs principaux:	Ewa Siucinska, Adam Hamed, Malgorzata Jasinska
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2014
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/0e58c20d110f419b975ffe717b937fb2
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spelling	oai:doaj.org-article:0e58c20d110f419b975ffe717b937fb22021-11-25T05:55:48ZIncreases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.1932-620310.1371/journal.pone.0110493https://doaj.org/article/0e58c20d110f419b975ffe717b937fb22014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110493https://doaj.org/toc/1932-6203Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS) with a tail shock (unconditioned stimulus, UCS) expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV of the barrel cortex. We have also shown that functional reorganization of the primary somatosensory cortex (S1) increases GABAergic markers in the hollows of "trained" barrels of the adult mouse. This study investigated how whisker-shock conditioning (CS+UCS) affected the expression of puncta of a high-affinity GABA plasma membrane transporter GAT-1 in the barrel cortex of mice 24 h after associative learning paradigm. We found that whisker-shock conditioning (CS+UCS) led to increase expression of neuronal and astroglial GAT-1 puncta in the "trained" row compared to controls: Pseudoconditioned, CS-only, UCS-only and Naïve animals. These findings suggest that fear conditioning specifically induces activation of systems regulating cellular levels of the inhibitory neurotransmitter GABA.Ewa SiucinskaAdam HamedMalgorzata JasinskaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110493 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ewa Siucinska Adam Hamed Malgorzata Jasinska Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
description	Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS) with a tail shock (unconditioned stimulus, UCS) expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV of the barrel cortex. We have also shown that functional reorganization of the primary somatosensory cortex (S1) increases GABAergic markers in the hollows of "trained" barrels of the adult mouse. This study investigated how whisker-shock conditioning (CS+UCS) affected the expression of puncta of a high-affinity GABA plasma membrane transporter GAT-1 in the barrel cortex of mice 24 h after associative learning paradigm. We found that whisker-shock conditioning (CS+UCS) led to increase expression of neuronal and astroglial GAT-1 puncta in the "trained" row compared to controls: Pseudoconditioned, CS-only, UCS-only and Naïve animals. These findings suggest that fear conditioning specifically induces activation of systems regulating cellular levels of the inhibitory neurotransmitter GABA.
format	article
author	Ewa Siucinska Adam Hamed Malgorzata Jasinska
author_facet	Ewa Siucinska Adam Hamed Malgorzata Jasinska
author_sort	Ewa Siucinska
title	Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_short	Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_full	Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_fullStr	Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_full_unstemmed	Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_sort	increases in the numerical density of gat-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0e58c20d110f419b975ffe717b937fb2
work_keys_str_mv	AT ewasiucinska increasesinthenumericaldensityofgat1positivepunctainthebarrelcortexofadultmiceafterfearconditioning AT adamhamed increasesinthenumericaldensityofgat1positivepunctainthebarrelcortexofadultmiceafterfearconditioning AT malgorzatajasinska increasesinthenumericaldensityofgat1positivepunctainthebarrelcortexofadultmiceafterfearconditioning
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Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.

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