Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.

Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS) with a tail shock (unconditioned stimulus, UCS) expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV...

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Autores principales: Ewa Siucinska, Adam Hamed, Malgorzata Jasinska
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Publicado: Public Library of Science (PLoS) 2014
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spelling oai:doaj.org-article:0e58c20d110f419b975ffe717b937fb22021-11-25T05:55:48ZIncreases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.1932-620310.1371/journal.pone.0110493https://doaj.org/article/0e58c20d110f419b975ffe717b937fb22014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110493https://doaj.org/toc/1932-6203Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS) with a tail shock (unconditioned stimulus, UCS) expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV of the barrel cortex. We have also shown that functional reorganization of the primary somatosensory cortex (S1) increases GABAergic markers in the hollows of "trained" barrels of the adult mouse. This study investigated how whisker-shock conditioning (CS+UCS) affected the expression of puncta of a high-affinity GABA plasma membrane transporter GAT-1 in the barrel cortex of mice 24 h after associative learning paradigm. We found that whisker-shock conditioning (CS+UCS) led to increase expression of neuronal and astroglial GAT-1 puncta in the "trained" row compared to controls: Pseudoconditioned, CS-only, UCS-only and Naïve animals. These findings suggest that fear conditioning specifically induces activation of systems regulating cellular levels of the inhibitory neurotransmitter GABA.Ewa SiucinskaAdam HamedMalgorzata JasinskaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110493 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ewa Siucinska
Adam Hamed
Malgorzata Jasinska
Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
description Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae (conditioned stimulus, CS) with a tail shock (unconditioned stimulus, UCS) expands the representation of "trained" vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV of the barrel cortex. We have also shown that functional reorganization of the primary somatosensory cortex (S1) increases GABAergic markers in the hollows of "trained" barrels of the adult mouse. This study investigated how whisker-shock conditioning (CS+UCS) affected the expression of puncta of a high-affinity GABA plasma membrane transporter GAT-1 in the barrel cortex of mice 24 h after associative learning paradigm. We found that whisker-shock conditioning (CS+UCS) led to increase expression of neuronal and astroglial GAT-1 puncta in the "trained" row compared to controls: Pseudoconditioned, CS-only, UCS-only and Naïve animals. These findings suggest that fear conditioning specifically induces activation of systems regulating cellular levels of the inhibitory neurotransmitter GABA.
format article
author Ewa Siucinska
Adam Hamed
Malgorzata Jasinska
author_facet Ewa Siucinska
Adam Hamed
Malgorzata Jasinska
author_sort Ewa Siucinska
title Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_short Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_full Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_fullStr Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_full_unstemmed Increases in the numerical density of GAT-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
title_sort increases in the numerical density of gat-1 positive puncta in the barrel cortex of adult mice after fear conditioning.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/0e58c20d110f419b975ffe717b937fb2
work_keys_str_mv AT ewasiucinska increasesinthenumericaldensityofgat1positivepunctainthebarrelcortexofadultmiceafterfearconditioning
AT adamhamed increasesinthenumericaldensityofgat1positivepunctainthebarrelcortexofadultmiceafterfearconditioning
AT malgorzatajasinska increasesinthenumericaldensityofgat1positivepunctainthebarrelcortexofadultmiceafterfearconditioning
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