Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats

Introduction: Parkinson’s Disease (PD) associates with changes in sex hormones; however, it remains unknown whether this is either a cause for or a result of the disease. To further evaluate it, we investigated if the development of 6-Hydroxydopamine (6-OHDA)-induced Parkinsonism changes the serum l...

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Autores principales:	Roghaieh Razaghi, Hossein Piri, Hanieh Jafari, Nafiseh Rastgoo, Mohammad Ali Hosseini, Hashem Haghdoost – Yazdi
Formato:	article
Lenguaje:	EN
Publicado:	Iran University of Medical Sciences 2021
Materias:	parkinson’s disease 6-hydroxydopamine testosterone prolactin dopaminergic Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/0e5b5e6372264dc2829ef011795af803
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spelling	oai:doaj.org-article:0e5b5e6372264dc2829ef011795af8032021-12-05T08:43:44ZEvaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats2008-126X2228-7442https://doaj.org/article/0e5b5e6372264dc2829ef011795af8032021-07-01T00:00:00Zhttp://bcn.iums.ac.ir/article-1-1556-en.htmlhttps://doaj.org/toc/2008-126Xhttps://doaj.org/toc/2228-7442Introduction: Parkinson’s Disease (PD) associates with changes in sex hormones; however, it remains unknown whether this is either a cause for or a result of the disease. To further evaluate it, we investigated if the development of 6-Hydroxydopamine (6-OHDA)-induced Parkinsonism changes the serum levels of testosterone and prolactin or not. Methods: 6-OHDA was injected into the medial forebrain bundle using stereotaxic surgery. The development of Parkinsonism was evaluated by apomorphine-induced rotational test and the immunofluorescence labeling of Dopaminergic (DA) neurons in substantia nigra. The necessary blood samples were collected before the toxin and in the third and sixth weeks afterward. The hormones levels were determined using Enzyme-Linked Immunosorbent Assay (ELISA) kits. Results: The severity of rotations was different among 6-OHDA-treated rats; accordingly, they were divided into two subgroups of severe and mild parkinsonian rats. The degeneration of DA neurons was observed in both subgroups; however, it was significantly less in the mild group. In the sixth week after the toxin, testosterone level increased but only in the severe subgroup. Prolactin increased in both subgroups in the third week after the toxin but returned to normal in the sixth week. There was no association between the pre-toxin levels of these hormones and the intensity of Parkinsonism. Conclusion: Our findings indicated that the development of 6-OHDA-induced Parkinsonism increases the serum levels of testosterone and prolactin. Increased prolactin occurred earlier and was observed in rats with less DA neuronal loss. Therefore, prolactin levels can predict the death of DA neurons before the clinical signs of PD were revealed.Roghaieh RazaghiHossein PiriHanieh JafariNafiseh RastgooMohammad Ali HosseiniHashem Haghdoost – YazdiIran University of Medical Sciencesarticleparkinson’s disease6-hydroxydopaminetestosteroneprolactindopaminergicNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBasic and Clinical Neuroscience, Vol 12, Iss 4, Pp 453-464 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	parkinson’s disease 6-hydroxydopamine testosterone prolactin dopaminergic Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	parkinson’s disease 6-hydroxydopamine testosterone prolactin dopaminergic Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Roghaieh Razaghi Hossein Piri Hanieh Jafari Nafiseh Rastgoo Mohammad Ali Hosseini Hashem Haghdoost – Yazdi Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats
description	Introduction: Parkinson’s Disease (PD) associates with changes in sex hormones; however, it remains unknown whether this is either a cause for or a result of the disease. To further evaluate it, we investigated if the development of 6-Hydroxydopamine (6-OHDA)-induced Parkinsonism changes the serum levels of testosterone and prolactin or not. Methods: 6-OHDA was injected into the medial forebrain bundle using stereotaxic surgery. The development of Parkinsonism was evaluated by apomorphine-induced rotational test and the immunofluorescence labeling of Dopaminergic (DA) neurons in substantia nigra. The necessary blood samples were collected before the toxin and in the third and sixth weeks afterward. The hormones levels were determined using Enzyme-Linked Immunosorbent Assay (ELISA) kits. Results: The severity of rotations was different among 6-OHDA-treated rats; accordingly, they were divided into two subgroups of severe and mild parkinsonian rats. The degeneration of DA neurons was observed in both subgroups; however, it was significantly less in the mild group. In the sixth week after the toxin, testosterone level increased but only in the severe subgroup. Prolactin increased in both subgroups in the third week after the toxin but returned to normal in the sixth week. There was no association between the pre-toxin levels of these hormones and the intensity of Parkinsonism. Conclusion: Our findings indicated that the development of 6-OHDA-induced Parkinsonism increases the serum levels of testosterone and prolactin. Increased prolactin occurred earlier and was observed in rats with less DA neuronal loss. Therefore, prolactin levels can predict the death of DA neurons before the clinical signs of PD were revealed.
format	article
author	Roghaieh Razaghi Hossein Piri Hanieh Jafari Nafiseh Rastgoo Mohammad Ali Hosseini Hashem Haghdoost – Yazdi
author_facet	Roghaieh Razaghi Hossein Piri Hanieh Jafari Nafiseh Rastgoo Mohammad Ali Hosseini Hashem Haghdoost – Yazdi
author_sort	Roghaieh Razaghi
title	Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats
title_short	Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats
title_full	Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats
title_fullStr	Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats
title_full_unstemmed	Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats
title_sort	evaluation of the association between serum levels of testosterone and prolactin with 6- hydroxydopamine- induced parkinsonism in male rats
publisher	Iran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/0e5b5e6372264dc2829ef011795af803
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Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine- Induced Parkinsonism in Male Rats

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