Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.

Flavobacterium psychrophilum is a bacterial species that represents one of the most important pathogens for aquaculture worldwide, especially for salmonids. To gain insights into the genetic basis of the natural resistance to F. psychrophilum, we selected homozygous clones of rainbow trout with cont...

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Autores principales: Christelle Langevin, Mar Blanco, Samuel A M Martin, Luc Jouneau, Jean-Francois Bernardet, Armel Houel, Aurélie Lunazzi, Eric Duchaud, Christian Michel, Edwige Quillet, Pierre Boudinot
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Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/0e5bb4245c9c4f47866bfb1d39d511c9
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spelling oai:doaj.org-article:0e5bb4245c9c4f47866bfb1d39d511c92021-11-18T07:15:33ZTranscriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.1932-620310.1371/journal.pone.0039126https://doaj.org/article/0e5bb4245c9c4f47866bfb1d39d511c92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22720048/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Flavobacterium psychrophilum is a bacterial species that represents one of the most important pathogens for aquaculture worldwide, especially for salmonids. To gain insights into the genetic basis of the natural resistance to F. psychrophilum, we selected homozygous clones of rainbow trout with contrasted susceptibility to the infection. We compared the transcriptional response to the bacteria in the pronephros of a susceptible and a resistant line by micro-array analysis five days after infection. While the basal transcriptome of healthy fish was significantly different in the resistant and susceptible lines, the transcriptome modifications induced by the bacteria involved essentially the same genes and pathways. The response to F. psychrophilum involved antimicrobial peptides, complement, and a number of enzymes and chemokines. The matrix metalloproteases mmp9 and mmp13 were among the most highly induced genes in both genetic backgrounds. Key genes of both pro- and anti-inflammatory response such as IL1 and IL10, were up-regulated with a greater magnitude in susceptible animals where the bacterial load was also much higher. While higher resistance to F. psychrophilum does not seem to be based on extensive differences in the orientation of the immune response, several genes including complement C3 showed stronger induction in the resistant fish. They may be important for the variation of susceptibility to the infection.Christelle LangevinMar BlancoSamuel A M MartinLuc JouneauJean-Francois BernardetArmel HouelAurélie LunazziEric DuchaudChristian MichelEdwige QuilletPierre BoudinotPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39126 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Christelle Langevin
Mar Blanco
Samuel A M Martin
Luc Jouneau
Jean-Francois Bernardet
Armel Houel
Aurélie Lunazzi
Eric Duchaud
Christian Michel
Edwige Quillet
Pierre Boudinot
Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.
description Flavobacterium psychrophilum is a bacterial species that represents one of the most important pathogens for aquaculture worldwide, especially for salmonids. To gain insights into the genetic basis of the natural resistance to F. psychrophilum, we selected homozygous clones of rainbow trout with contrasted susceptibility to the infection. We compared the transcriptional response to the bacteria in the pronephros of a susceptible and a resistant line by micro-array analysis five days after infection. While the basal transcriptome of healthy fish was significantly different in the resistant and susceptible lines, the transcriptome modifications induced by the bacteria involved essentially the same genes and pathways. The response to F. psychrophilum involved antimicrobial peptides, complement, and a number of enzymes and chemokines. The matrix metalloproteases mmp9 and mmp13 were among the most highly induced genes in both genetic backgrounds. Key genes of both pro- and anti-inflammatory response such as IL1 and IL10, were up-regulated with a greater magnitude in susceptible animals where the bacterial load was also much higher. While higher resistance to F. psychrophilum does not seem to be based on extensive differences in the orientation of the immune response, several genes including complement C3 showed stronger induction in the resistant fish. They may be important for the variation of susceptibility to the infection.
format article
author Christelle Langevin
Mar Blanco
Samuel A M Martin
Luc Jouneau
Jean-Francois Bernardet
Armel Houel
Aurélie Lunazzi
Eric Duchaud
Christian Michel
Edwige Quillet
Pierre Boudinot
author_facet Christelle Langevin
Mar Blanco
Samuel A M Martin
Luc Jouneau
Jean-Francois Bernardet
Armel Houel
Aurélie Lunazzi
Eric Duchaud
Christian Michel
Edwige Quillet
Pierre Boudinot
author_sort Christelle Langevin
title Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.
title_short Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.
title_full Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.
title_fullStr Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.
title_full_unstemmed Transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen Flavobacterium psychrophilum.
title_sort transcriptional responses of resistant and susceptible fish clones to the bacterial pathogen flavobacterium psychrophilum.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/0e5bb4245c9c4f47866bfb1d39d511c9
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