Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study

Abstract Fading puppy syndrome (FPS) is a fatal condition in neonatal dogs. Intestinal microbial alterations, although never investigated, may be involved in its pathophysiology. The study examined the occurrence of FPS and its associations with dam, puppy, and husbandry characteristics, compared th...

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Autores principales:	Smadar Tal, Evgenii Tikhonov, Itamar Aroch, Lior Hefetz, Sondra Turjeman, Omry Koren, Sharon Kuzi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/0e65942877a242e4839955373bec6f75
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spelling	oai:doaj.org-article:0e65942877a242e4839955373bec6f752021-12-02T18:02:50ZDevelopmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study10.1038/s41522-021-00222-72055-5008https://doaj.org/article/0e65942877a242e4839955373bec6f752021-06-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00222-7https://doaj.org/toc/2055-5008Abstract Fading puppy syndrome (FPS) is a fatal condition in neonatal dogs. Intestinal microbial alterations, although never investigated, may be involved in its pathophysiology. The study examined the occurrence of FPS and its associations with dam, puppy, and husbandry characteristics, compared the intestinal microbial diversity of healthy puppies and those with FPS, and examined whether intestinal microbiomes are predictive of FPS. Day 1 and 8 post-partum (PP) rectal swabs were collected from healthy puppies and puppies which later developed FPS. Microbial compositional structure, including alpha and beta diversities and relative abundance of specific taxa were compared between groups, and microbial data was applied to a machine-learning model to assess the predictive performance of microbial indices of FPS or death. FPS occurred in 22/165 puppies (13%), with a 100% mortality rate. FPS was associated (P < 0.001) with decreased Day 1 PP puppy activity. Day 1 (P = 0.003) and 8 (P = 0.005) PP rectal beta diversities were different in puppies with FPS vs healthy ones. Increased Proteobacteria/Firmicutes ratio, increased relative abundance of Pasteurellaceae, and decreased relative abundance of Clostridia and Enterococcus were associated with FPS. A machine-learning model showed that Day 1 PP rectal microbiome composition accurately predicted FPS-related death. We found that specific rectal microbial phenotypes are associated with FPS, reflecting the significant role of microbiome alterations in this phenomenon. These findings may serve as useful microbial indices for early diagnosis of puppies at risk of FPS and may provide specific therapeutic targets.Smadar TalEvgenii TikhonovItamar ArochLior HefetzSondra TurjemanOmry KorenSharon KuziNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Smadar Tal Evgenii Tikhonov Itamar Aroch Lior Hefetz Sondra Turjeman Omry Koren Sharon Kuzi Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
description	Abstract Fading puppy syndrome (FPS) is a fatal condition in neonatal dogs. Intestinal microbial alterations, although never investigated, may be involved in its pathophysiology. The study examined the occurrence of FPS and its associations with dam, puppy, and husbandry characteristics, compared the intestinal microbial diversity of healthy puppies and those with FPS, and examined whether intestinal microbiomes are predictive of FPS. Day 1 and 8 post-partum (PP) rectal swabs were collected from healthy puppies and puppies which later developed FPS. Microbial compositional structure, including alpha and beta diversities and relative abundance of specific taxa were compared between groups, and microbial data was applied to a machine-learning model to assess the predictive performance of microbial indices of FPS or death. FPS occurred in 22/165 puppies (13%), with a 100% mortality rate. FPS was associated (P < 0.001) with decreased Day 1 PP puppy activity. Day 1 (P = 0.003) and 8 (P = 0.005) PP rectal beta diversities were different in puppies with FPS vs healthy ones. Increased Proteobacteria/Firmicutes ratio, increased relative abundance of Pasteurellaceae, and decreased relative abundance of Clostridia and Enterococcus were associated with FPS. A machine-learning model showed that Day 1 PP rectal microbiome composition accurately predicted FPS-related death. We found that specific rectal microbial phenotypes are associated with FPS, reflecting the significant role of microbiome alterations in this phenomenon. These findings may serve as useful microbial indices for early diagnosis of puppies at risk of FPS and may provide specific therapeutic targets.
format	article
author	Smadar Tal Evgenii Tikhonov Itamar Aroch Lior Hefetz Sondra Turjeman Omry Koren Sharon Kuzi
author_facet	Smadar Tal Evgenii Tikhonov Itamar Aroch Lior Hefetz Sondra Turjeman Omry Koren Sharon Kuzi
author_sort	Smadar Tal
title	Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
title_short	Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
title_full	Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
title_fullStr	Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
title_full_unstemmed	Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
title_sort	developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0e65942877a242e4839955373bec6f75
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Developmental intestinal microbiome alterations in canine fading puppy syndrome: a prospective observational study

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