Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of Rhododendron delavayi Franch

Rhododendron delavayi Franch. is a famous ornamental plant. However, seasonal drought caused by a monsoon climate seriously limits its growth and flowering performance in urban gardens. Our previous study has found that brassinosteroids (BRs) can improve the tolerance of ...

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Autores principales:	Yan-Fei Cai, Lu Zhang, Lv-Chun Peng, Shi-Feng Li, Jie Song, Wei-Jia Xie, Ji-Hua Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	brassinosteroids data-independent acquisition drought stress proteomic analysis <i>Rhododendron delavayi</i> Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0e6b3d3d50d44819b2301e95be0335f2
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spelling	oai:doaj.org-article:0e6b3d3d50d44819b2301e95be0335f22021-11-25T17:47:41ZKey Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch10.3390/horticulturae71105012311-7524https://doaj.org/article/0e6b3d3d50d44819b2301e95be0335f22021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/501https://doaj.org/toc/2311-7524<i>Rhododendron delavayi</i> Franch. is a famous ornamental plant. However, seasonal drought caused by a monsoon climate seriously limits its growth and flowering performance in urban gardens. Our previous study has found that brassinosteroids (BRs) can improve the tolerance of <i>R. delavayi</i> to drought stress. Here, we employed a data-independent acquisition (DIA) approach to compare the protein expression profiles under drought treatment (D) and pre-treatment with BR before drought treatment (BR). With an increase in drought stress, the net photosynthetic rate, stomatal conductance, and transpiration rate in the BR treatment showed more stable changes that were significantly higher than those in the D treatment. However, the contents of malondialdehyde, soluble sugar, soluble protein, and the activity of superoxide dismutase (SOD), peroxidase, and catalase showed opposite trends. The pre-treatment with BR alleviated the negative effect of drought stress on the photosynthetic performance of <i>R. delavayi.</i> A total of 3453 differentially expressed proteins (DEPs) were identified, and 683 DEPs were significantly expressed in the D and BR treatments. The DEPs uniquely expressed in the BR treatment participated in the pathways of “ribosome”, “ether lipid metabolism”, “photosynthesis”, and “oxidative phosphorylation”. The improvement effect of the BR treatment on the drought tolerance of <i>R. delavayi</i> was mainly attributed to improved photosynthesis by alleviating stomatal closure and oxidative stress, maintaining the integrity and stability of the ribosomal complex to mediate protein synthesis and the balance between energy metabolism and carbon metabolism. Our study presents a comprehensive understanding of the key proteins and metabolic pathways related to the response of <i>R. delavayi</i> to drought and will contribute to the breeding of drought-tolerant rhododendrons.Yan-Fei CaiLu ZhangLv-Chun PengShi-Feng LiJie SongWei-Jia XieJi-Hua WangMDPI AGarticlebrassinosteroidsdata-independent acquisitiondrought stressproteomic analysis<i>Rhododendron delavayi</i>Plant cultureSB1-1110ENHorticulturae, Vol 7, Iss 501, p 501 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	brassinosteroids data-independent acquisition drought stress proteomic analysis <i>Rhododendron delavayi</i> Plant culture SB1-1110
spellingShingle	brassinosteroids data-independent acquisition drought stress proteomic analysis <i>Rhododendron delavayi</i> Plant culture SB1-1110 Yan-Fei Cai Lu Zhang Lv-Chun Peng Shi-Feng Li Jie Song Wei-Jia Xie Ji-Hua Wang Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch
description	<i>Rhododendron delavayi</i> Franch. is a famous ornamental plant. However, seasonal drought caused by a monsoon climate seriously limits its growth and flowering performance in urban gardens. Our previous study has found that brassinosteroids (BRs) can improve the tolerance of <i>R. delavayi</i> to drought stress. Here, we employed a data-independent acquisition (DIA) approach to compare the protein expression profiles under drought treatment (D) and pre-treatment with BR before drought treatment (BR). With an increase in drought stress, the net photosynthetic rate, stomatal conductance, and transpiration rate in the BR treatment showed more stable changes that were significantly higher than those in the D treatment. However, the contents of malondialdehyde, soluble sugar, soluble protein, and the activity of superoxide dismutase (SOD), peroxidase, and catalase showed opposite trends. The pre-treatment with BR alleviated the negative effect of drought stress on the photosynthetic performance of <i>R. delavayi.</i> A total of 3453 differentially expressed proteins (DEPs) were identified, and 683 DEPs were significantly expressed in the D and BR treatments. The DEPs uniquely expressed in the BR treatment participated in the pathways of “ribosome”, “ether lipid metabolism”, “photosynthesis”, and “oxidative phosphorylation”. The improvement effect of the BR treatment on the drought tolerance of <i>R. delavayi</i> was mainly attributed to improved photosynthesis by alleviating stomatal closure and oxidative stress, maintaining the integrity and stability of the ribosomal complex to mediate protein synthesis and the balance between energy metabolism and carbon metabolism. Our study presents a comprehensive understanding of the key proteins and metabolic pathways related to the response of <i>R. delavayi</i> to drought and will contribute to the breeding of drought-tolerant rhododendrons.
format	article
author	Yan-Fei Cai Lu Zhang Lv-Chun Peng Shi-Feng Li Jie Song Wei-Jia Xie Ji-Hua Wang
author_facet	Yan-Fei Cai Lu Zhang Lv-Chun Peng Shi-Feng Li Jie Song Wei-Jia Xie Ji-Hua Wang
author_sort	Yan-Fei Cai
title	Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch
title_short	Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch
title_full	Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch
title_fullStr	Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch
title_full_unstemmed	Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch
title_sort	key proteins and metabolic pathways involved in 24-epibrasionlide improving drought tolerance of <i>rhododendron delavayi</i> franch
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0e6b3d3d50d44819b2301e95be0335f2
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Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of <i>Rhododendron delavayi</i> Franch

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