Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes

Three silver(I) dipeptide complexes [Ag(GlyGly)]n(NO3)n (AgGlyGly), [Ag2(GlyAla)(NO3)2]n<...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gabriela Kuzderová, Michaela Rendošová, Róbert Gyepes, Simona Sovová, Danica Sabolová, Mária Vilková, Petra Olejníková, Ivana Bačová, Simonida Stokič, Martin Kello, Zuzana Vargová
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	silver(I) complexes dipeptide crystal structure stability antimicrobial activity anticancer activity Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/0e7340056ac4427f81f8f2632b13c4fe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0e7340056ac4427f81f8f2632b13c4fe
record_format	dspace
spelling	oai:doaj.org-article:0e7340056ac4427f81f8f2632b13c4fe2021-11-11T18:22:09ZAntimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes10.3390/molecules262163351420-3049https://doaj.org/article/0e7340056ac4427f81f8f2632b13c4fe2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6335https://doaj.org/toc/1420-3049Three silver(I) dipeptide complexes [Ag(GlyGly)]<i><sub>n</sub></i>(NO<sub>3</sub>)<i><sub>n</sub></i> (AgGlyGly), [Ag<sub>2</sub>(GlyAla)(NO<sub>3</sub>)<sub>2</sub>]<i><sub>n</sub></i> (AgGlyAla) and [Ag<sub>2</sub>(HGlyAsp)(NO<sub>3</sub>)]<i><sub>n</sub></i> (AgGlyAsp) were prepared, investigated and characterized by vibrational spectroscopy (mid-IR), elemental and thermogravimetric analysis and mass spectrometry. For AgGlyGly, X-ray crystallography was also performed. Their stability in biological testing media was verified by time-dependent NMR measurements. Their in vitro antimicrobial activity was evaluated against selected pathogenic microorganisms. Moreover, the influence of silver(I) dipeptide complexes on microbial film formation was described. Further, the cytotoxicity of the complexes against selected cancer cells (BLM, MDA-MB-231, HeLa, HCT116, MCF-7 and Jurkat) and fibroblasts (BJ-5ta) using a colorimetric MTS assay was tested, and the selectivity index (SI) was identified. The mechanism of action of Ag(I) dipeptide complexes was elucidated and discussed by the study in terms of their binding affinity toward the CT DNA, the ability to cleave the DNA and the ability to influence numbers of cells within each cell cycle phase. The new silver(I) dipeptide complexes are able to bind into DNA by noncovalent interaction, and the topoisomerase I inhibition study showed that the studied complexes inhibit its activity at a concentration of 15 μM.Gabriela KuzderováMichaela RendošováRóbert GyepesSimona SovováDanica SabolováMária VilkováPetra OlejníkováIvana BačováSimonida StokičMartin KelloZuzana VargováMDPI AGarticlesilver(I) complexesdipeptidecrystal structurestabilityantimicrobial activityanticancer activityOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6335, p 6335 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver(I) complexes dipeptide crystal structure stability antimicrobial activity anticancer activity Organic chemistry QD241-441
spellingShingle	silver(I) complexes dipeptide crystal structure stability antimicrobial activity anticancer activity Organic chemistry QD241-441 Gabriela Kuzderová Michaela Rendošová Róbert Gyepes Simona Sovová Danica Sabolová Mária Vilková Petra Olejníková Ivana Bačová Simonida Stokič Martin Kello Zuzana Vargová Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
description	Three silver(I) dipeptide complexes [Ag(GlyGly)]<i><sub>n</sub></i>(NO<sub>3</sub>)<i><sub>n</sub></i> (AgGlyGly), [Ag<sub>2</sub>(GlyAla)(NO<sub>3</sub>)<sub>2</sub>]<i><sub>n</sub></i> (AgGlyAla) and [Ag<sub>2</sub>(HGlyAsp)(NO<sub>3</sub>)]<i><sub>n</sub></i> (AgGlyAsp) were prepared, investigated and characterized by vibrational spectroscopy (mid-IR), elemental and thermogravimetric analysis and mass spectrometry. For AgGlyGly, X-ray crystallography was also performed. Their stability in biological testing media was verified by time-dependent NMR measurements. Their in vitro antimicrobial activity was evaluated against selected pathogenic microorganisms. Moreover, the influence of silver(I) dipeptide complexes on microbial film formation was described. Further, the cytotoxicity of the complexes against selected cancer cells (BLM, MDA-MB-231, HeLa, HCT116, MCF-7 and Jurkat) and fibroblasts (BJ-5ta) using a colorimetric MTS assay was tested, and the selectivity index (SI) was identified. The mechanism of action of Ag(I) dipeptide complexes was elucidated and discussed by the study in terms of their binding affinity toward the CT DNA, the ability to cleave the DNA and the ability to influence numbers of cells within each cell cycle phase. The new silver(I) dipeptide complexes are able to bind into DNA by noncovalent interaction, and the topoisomerase I inhibition study showed that the studied complexes inhibit its activity at a concentration of 15 μM.
format	article
author	Gabriela Kuzderová Michaela Rendošová Róbert Gyepes Simona Sovová Danica Sabolová Mária Vilková Petra Olejníková Ivana Bačová Simonida Stokič Martin Kello Zuzana Vargová
author_facet	Gabriela Kuzderová Michaela Rendošová Róbert Gyepes Simona Sovová Danica Sabolová Mária Vilková Petra Olejníková Ivana Bačová Simonida Stokič Martin Kello Zuzana Vargová
author_sort	Gabriela Kuzderová
title	Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
title_short	Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
title_full	Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
title_fullStr	Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
title_full_unstemmed	Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
title_sort	antimicrobial and anticancer application of silver(i) dipeptide complexes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0e7340056ac4427f81f8f2632b13c4fe
work_keys_str_mv	AT gabrielakuzderova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT michaelarendosova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT robertgyepes antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT simonasovova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT danicasabolova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT mariavilkova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT petraolejnikova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT ivanabacova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT simonidastokic antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT martinkello antimicrobialandanticancerapplicationofsilveridipeptidecomplexes AT zuzanavargova antimicrobialandanticancerapplicationofsilveridipeptidecomplexes
_version_	1718431805180215296

Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes

Ejemplares similares