Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni

Abstract Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide...

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Autores principales:	Mariana Boroni, Michael Sammeth, Sandra Grossi Gava, Natasha Andressa Nogueira Jorge, Andréa Mara Macedo, Carlos Renato Machado, Marina Moraes Mourão, Glória Regina Franco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e756365d71143b4b464e9bcddd7f1ab
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spelling	oai:doaj.org-article:0e756365d71143b4b464e9bcddd7f1ab2021-12-02T15:08:06ZLandscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni10.1038/s41598-018-22093-32045-2322https://doaj.org/article/0e756365d71143b4b464e9bcddd7f1ab2018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22093-3https://doaj.org/toc/2045-2322Abstract Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide spectrum of functional classes, which underlines the SLTS as a ubiquitous mechanism in the parasite. Also, SLTS gene expression levels span several orders of magnitude, showing that SLTS frequency is not determined by the expression level of the target gene, but by the presence of particular gene features facilitating or hindering the trans-splicing mechanism. Our in-depth investigation of SLTS events demonstrates widespread alternative trans-splicing (ATS) acceptor sites occurring in different regions along the entire gene body, highlighting another important role of SLTS generating alternative RNA isoforms in the parasite, besides the polycistron resolution. Particularly for introns where SLTS directly competes for the same acceptor substrate with cis-splicing, we identified for the first time additional and important features that might determine the type of splicing. Our study substantially extends the current knowledge of RNA processing by SLTS in S. mansoni, and provide basis for future studies on the trans-splicing mechanism in other eukaryotes.Mariana BoroniMichael SammethSandra Grossi GavaNatasha Andressa Nogueira JorgeAndréa Mara MacedoCarlos Renato MachadoMarina Moraes MourãoGlória Regina FrancoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Boroni Michael Sammeth Sandra Grossi Gava Natasha Andressa Nogueira Jorge Andréa Mara Macedo Carlos Renato Machado Marina Moraes Mourão Glória Regina Franco Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
description	Abstract Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide spectrum of functional classes, which underlines the SLTS as a ubiquitous mechanism in the parasite. Also, SLTS gene expression levels span several orders of magnitude, showing that SLTS frequency is not determined by the expression level of the target gene, but by the presence of particular gene features facilitating or hindering the trans-splicing mechanism. Our in-depth investigation of SLTS events demonstrates widespread alternative trans-splicing (ATS) acceptor sites occurring in different regions along the entire gene body, highlighting another important role of SLTS generating alternative RNA isoforms in the parasite, besides the polycistron resolution. Particularly for introns where SLTS directly competes for the same acceptor substrate with cis-splicing, we identified for the first time additional and important features that might determine the type of splicing. Our study substantially extends the current knowledge of RNA processing by SLTS in S. mansoni, and provide basis for future studies on the trans-splicing mechanism in other eukaryotes.
format	article
author	Mariana Boroni Michael Sammeth Sandra Grossi Gava Natasha Andressa Nogueira Jorge Andréa Mara Macedo Carlos Renato Machado Marina Moraes Mourão Glória Regina Franco
author_facet	Mariana Boroni Michael Sammeth Sandra Grossi Gava Natasha Andressa Nogueira Jorge Andréa Mara Macedo Carlos Renato Machado Marina Moraes Mourão Glória Regina Franco
author_sort	Mariana Boroni
title	Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
title_short	Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
title_full	Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
title_fullStr	Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
title_full_unstemmed	Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni
title_sort	landscape of the spliced leader trans-splicing mechanism in schistosoma mansoni
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0e756365d71143b4b464e9bcddd7f1ab
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Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni

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