Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

CRISPR array generation is difficult due to reoccurring repeat sequences. Here the authors present CRATES—a modular, one-pot assembly method—and demonstrate the creation of arrays for Cas9, Cas12a and Cas13a for cell-free, bacterial, yeast and mammalian systems.

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Autores principales:	Chunyu Liao, Fani Ttofali, Rebecca A. Slotkowski, Steven R. Denny, Taylor D. Cecil, Ryan T. Leenay, Albert J. Keung, Chase L. Beisel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0e7bb4e3bb974825935f7c719c17076e
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spelling	oai:doaj.org-article:0e7bb4e3bb974825935f7c719c17076e2021-12-02T15:35:27ZModular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis10.1038/s41467-019-10747-32041-1723https://doaj.org/article/0e7bb4e3bb974825935f7c719c17076e2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10747-3https://doaj.org/toc/2041-1723CRISPR array generation is difficult due to reoccurring repeat sequences. Here the authors present CRATES—a modular, one-pot assembly method—and demonstrate the creation of arrays for Cas9, Cas12a and Cas13a for cell-free, bacterial, yeast and mammalian systems.Chunyu LiaoFani TtofaliRebecca A. SlotkowskiSteven R. DennyTaylor D. CecilRyan T. LeenayAlbert J. KeungChase L. BeiselNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Chunyu Liao Fani Ttofali Rebecca A. Slotkowski Steven R. Denny Taylor D. Cecil Ryan T. Leenay Albert J. Keung Chase L. Beisel Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
description	CRISPR array generation is difficult due to reoccurring repeat sequences. Here the authors present CRATES—a modular, one-pot assembly method—and demonstrate the creation of arrays for Cas9, Cas12a and Cas13a for cell-free, bacterial, yeast and mammalian systems.
format	article
author	Chunyu Liao Fani Ttofali Rebecca A. Slotkowski Steven R. Denny Taylor D. Cecil Ryan T. Leenay Albert J. Keung Chase L. Beisel
author_facet	Chunyu Liao Fani Ttofali Rebecca A. Slotkowski Steven R. Denny Taylor D. Cecil Ryan T. Leenay Albert J. Keung Chase L. Beisel
author_sort	Chunyu Liao
title	Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
title_short	Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
title_full	Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
title_fullStr	Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
title_full_unstemmed	Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
title_sort	modular one-pot assembly of crispr arrays enables library generation and reveals factors influencing crrna biogenesis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/0e7bb4e3bb974825935f7c719c17076e
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_version_	1718386514792022016

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

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