Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

CRISPR array generation is difficult due to reoccurring repeat sequences. Here the authors present CRATES—a modular, one-pot assembly method—and demonstrate the creation of arrays for Cas9, Cas12a and Cas13a for cell-free, bacterial, yeast and mammalian systems.

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Detalles Bibliográficos
Autores principales: Chunyu Liao, Fani Ttofali, Rebecca A. Slotkowski, Steven R. Denny, Taylor D. Cecil, Ryan T. Leenay, Albert J. Keung, Chase L. Beisel
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
Science
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Acceso en línea:https://doaj.org/article/0e7bb4e3bb974825935f7c719c17076e
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