Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.

The siRNA pathway is an essential antiviral mechanism in insects. Whether other RNA interference pathways are involved in antiviral defense remains unclear. Here, we report in cells derived from the two main vectors for arboviruses, Aedes albopictus and Aedes aegypti, the production of viral small R...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicolas Vodovar, Alfred W Bronkhorst, Koen W R van Cleef, Pascal Miesen, Hervé Blanc, Ronald P van Rij, Maria-Carla Saleh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e7c276b9e784e69b906d3688485ff91
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0e7c276b9e784e69b906d3688485ff91
record_format	dspace
spelling	oai:doaj.org-article:0e7c276b9e784e69b906d3688485ff912021-11-18T07:29:25ZArbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.1932-620310.1371/journal.pone.0030861https://doaj.org/article/0e7c276b9e784e69b906d3688485ff912012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22292064/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The siRNA pathway is an essential antiviral mechanism in insects. Whether other RNA interference pathways are involved in antiviral defense remains unclear. Here, we report in cells derived from the two main vectors for arboviruses, Aedes albopictus and Aedes aegypti, the production of viral small RNAs that exhibit the hallmarks of ping-pong derived piwi-associated RNAs (piRNAs) after infection with positive or negative sense RNA viruses. Furthermore, these cells produce endogenous piRNAs that mapped to transposable elements. Our results show that these mosquito cells can initiate de novo piRNA production and recapitulate the ping-pong dependent piRNA pathway upon viral infection. The mechanism of viral-piRNA production is discussed.Nicolas VodovarAlfred W BronkhorstKoen W R van CleefPascal MiesenHervé BlancRonald P van RijMaria-Carla SalehPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30861 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolas Vodovar Alfred W Bronkhorst Koen W R van Cleef Pascal Miesen Hervé Blanc Ronald P van Rij Maria-Carla Saleh Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
description	The siRNA pathway is an essential antiviral mechanism in insects. Whether other RNA interference pathways are involved in antiviral defense remains unclear. Here, we report in cells derived from the two main vectors for arboviruses, Aedes albopictus and Aedes aegypti, the production of viral small RNAs that exhibit the hallmarks of ping-pong derived piwi-associated RNAs (piRNAs) after infection with positive or negative sense RNA viruses. Furthermore, these cells produce endogenous piRNAs that mapped to transposable elements. Our results show that these mosquito cells can initiate de novo piRNA production and recapitulate the ping-pong dependent piRNA pathway upon viral infection. The mechanism of viral-piRNA production is discussed.
format	article
author	Nicolas Vodovar Alfred W Bronkhorst Koen W R van Cleef Pascal Miesen Hervé Blanc Ronald P van Rij Maria-Carla Saleh
author_facet	Nicolas Vodovar Alfred W Bronkhorst Koen W R van Cleef Pascal Miesen Hervé Blanc Ronald P van Rij Maria-Carla Saleh
author_sort	Nicolas Vodovar
title	Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
title_short	Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
title_full	Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
title_fullStr	Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
title_full_unstemmed	Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.
title_sort	arbovirus-derived pirnas exhibit a ping-pong signature in mosquito cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0e7c276b9e784e69b906d3688485ff91
work_keys_str_mv	AT nicolasvodovar arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells AT alfredwbronkhorst arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells AT koenwrvancleef arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells AT pascalmiesen arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells AT herveblanc arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells AT ronaldpvanrij arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells AT mariacarlasaleh arbovirusderivedpirnasexhibitapingpongsignatureinmosquitocells
_version_	1718423330959130624

Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.

Ejemplares similares