Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.

Recent studies have shown that adipose-derived stromal/stem cells (ASCs) contain phenotypically and functionally heterogeneous subpopulations of cells, but their developmental origin and their relative differentiation potential remain elusive. In the present study, we aimed at investigating how and...

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Autores principales:	Yoshihiro Sowa, Tetsuya Imura, Toshiaki Numajiri, Kosuke Takeda, Yo Mabuchi, Yumi Matsuzaki, Kenichi Nishino
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e7cc83d44514f69a61798326f8d62ec
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spelling	oai:doaj.org-article:0e7cc83d44514f69a61798326f8d62ec2021-11-18T08:39:23ZAdipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.1932-620310.1371/journal.pone.0084206https://doaj.org/article/0e7cc83d44514f69a61798326f8d62ec2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391913/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies have shown that adipose-derived stromal/stem cells (ASCs) contain phenotypically and functionally heterogeneous subpopulations of cells, but their developmental origin and their relative differentiation potential remain elusive. In the present study, we aimed at investigating how and to what extent the neural crest contributes to ASCs using Cre-loxP-mediated fate mapping. ASCs harvested from subcutaneous fat depots of either adult P0-Cre/or Wnt1-Cre/Floxed-reporter mice contained a few neural crest-derived ASCs (NCDASCs). This subpopulation of cells was successfully expanded in vitro under standard culture conditions and their growth rate was comparable to non-neural crest derivatives. Although NCDASCs were positive for several mesenchymal stem cell markers as non-neural crest derivatives, they exhibited a unique bipolar or multipolar morphology with higher expression of markers for both neural crest progenitors (p75NTR, Nestin, and Sox2) and preadipocytes (CD24, CD34, S100, Pref-1, GATA2, and C/EBP-delta). NCDASCs were able to differentiate into adipocytes with high efficiency but their osteogenic and chondrogenic potential was markedly attenuated, indicating their commitment to adipogenesis. In vivo, a very small proportion of adipocytes were originated from the neural crest. In addition, p75NTR-positive neural crest-derived cells were identified along the vessels within the subcutaneous adipose tissue, but they were negative for mural and endothelial markers. These results demonstrate that ASCs contain neural crest-derived adipocyte-restricted progenitors whose phenotype is distinct from that of non-neural crest derivatives.Yoshihiro SowaTetsuya ImuraToshiaki NumajiriKosuke TakedaYo MabuchiYumi MatsuzakiKenichi NishinoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84206 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshihiro Sowa Tetsuya Imura Toshiaki Numajiri Kosuke Takeda Yo Mabuchi Yumi Matsuzaki Kenichi Nishino Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
description	Recent studies have shown that adipose-derived stromal/stem cells (ASCs) contain phenotypically and functionally heterogeneous subpopulations of cells, but their developmental origin and their relative differentiation potential remain elusive. In the present study, we aimed at investigating how and to what extent the neural crest contributes to ASCs using Cre-loxP-mediated fate mapping. ASCs harvested from subcutaneous fat depots of either adult P0-Cre/or Wnt1-Cre/Floxed-reporter mice contained a few neural crest-derived ASCs (NCDASCs). This subpopulation of cells was successfully expanded in vitro under standard culture conditions and their growth rate was comparable to non-neural crest derivatives. Although NCDASCs were positive for several mesenchymal stem cell markers as non-neural crest derivatives, they exhibited a unique bipolar or multipolar morphology with higher expression of markers for both neural crest progenitors (p75NTR, Nestin, and Sox2) and preadipocytes (CD24, CD34, S100, Pref-1, GATA2, and C/EBP-delta). NCDASCs were able to differentiate into adipocytes with high efficiency but their osteogenic and chondrogenic potential was markedly attenuated, indicating their commitment to adipogenesis. In vivo, a very small proportion of adipocytes were originated from the neural crest. In addition, p75NTR-positive neural crest-derived cells were identified along the vessels within the subcutaneous adipose tissue, but they were negative for mural and endothelial markers. These results demonstrate that ASCs contain neural crest-derived adipocyte-restricted progenitors whose phenotype is distinct from that of non-neural crest derivatives.
format	article
author	Yoshihiro Sowa Tetsuya Imura Toshiaki Numajiri Kosuke Takeda Yo Mabuchi Yumi Matsuzaki Kenichi Nishino
author_facet	Yoshihiro Sowa Tetsuya Imura Toshiaki Numajiri Kosuke Takeda Yo Mabuchi Yumi Matsuzaki Kenichi Nishino
author_sort	Yoshihiro Sowa
title	Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
title_short	Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
title_full	Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
title_fullStr	Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
title_full_unstemmed	Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
title_sort	adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0e7cc83d44514f69a61798326f8d62ec
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Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.

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