Cryo-Gel embedding compound for renal biopsy biobanking

Abstract Optimal preservation and biobanking of renal tissue is vital for good diagnostics and subsequent research. Optimal cutting temperature (OCT) compound is a commonly used embedding medium for freezing tissue samples. However, due to interfering polymers in OCT, analysis as mass spectrometry (...

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Autores principales: Malou L. H. Snijders, Marina Zajec, Laurens A. J. Walter, Remco M. A. A. de Louw, Monique H. A. Oomen, Shazia Arshad, Thierry P. P. van den Bosch, Lennard J. M. Dekker, Michail Doukas, Theo M. Luider, Peter H. J. Riegman, Folkert J. van Kemenade, Marian C. Clahsen-van Groningen
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Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/0e7d90e3e42941199e6d89072dc636f0
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spelling oai:doaj.org-article:0e7d90e3e42941199e6d89072dc636f02021-12-02T16:08:27ZCryo-Gel embedding compound for renal biopsy biobanking10.1038/s41598-019-51962-82045-2322https://doaj.org/article/0e7d90e3e42941199e6d89072dc636f02019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51962-8https://doaj.org/toc/2045-2322Abstract Optimal preservation and biobanking of renal tissue is vital for good diagnostics and subsequent research. Optimal cutting temperature (OCT) compound is a commonly used embedding medium for freezing tissue samples. However, due to interfering polymers in OCT, analysis as mass spectrometry (MS) is difficult. We investigated if the replacement of OCT with Cryo-Gel as embedding compound for renal biopsies would enable proteomics and not disturb other common techniques used in tissue diagnostics and research. For the present study, fresh renal samples were snap-frozen using Cryo-Gel, OCT and without embedding compound and evaluated using different techniques. In addition, tissue samples from normal spleen, skin, liver and colon were analyzed. Cryo-Gel embedded tissues showed good morphological preservation and no interference in immunohistochemical or immunofluorescent investigations. The quality of extracted RNA and DNA was good. The number of proteins identified using MS was similar between Cryo-Gel embedded samples, samples without embedding compound and OCT embedded samples. However, polymers in the OCT disturbed the signal in the MS, while this was not observed in the Cryo-Gel embedded samples. We conclude that embedding of renal biopsies in Cryo-Gel is an excellent and preferable alternative for OCT compound for both diagnostic and research purposes, especially in those cases where proteomic analysis might be necessary.Malou L. H. SnijdersMarina ZajecLaurens A. J. WalterRemco M. A. A. de LouwMonique H. A. OomenShazia ArshadThierry P. P. van den BoschLennard J. M. DekkerMichail DoukasTheo M. LuiderPeter H. J. RiegmanFolkert J. van KemenadeMarian C. Clahsen-van GroningenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Malou L. H. Snijders
Marina Zajec
Laurens A. J. Walter
Remco M. A. A. de Louw
Monique H. A. Oomen
Shazia Arshad
Thierry P. P. van den Bosch
Lennard J. M. Dekker
Michail Doukas
Theo M. Luider
Peter H. J. Riegman
Folkert J. van Kemenade
Marian C. Clahsen-van Groningen
Cryo-Gel embedding compound for renal biopsy biobanking
description Abstract Optimal preservation and biobanking of renal tissue is vital for good diagnostics and subsequent research. Optimal cutting temperature (OCT) compound is a commonly used embedding medium for freezing tissue samples. However, due to interfering polymers in OCT, analysis as mass spectrometry (MS) is difficult. We investigated if the replacement of OCT with Cryo-Gel as embedding compound for renal biopsies would enable proteomics and not disturb other common techniques used in tissue diagnostics and research. For the present study, fresh renal samples were snap-frozen using Cryo-Gel, OCT and without embedding compound and evaluated using different techniques. In addition, tissue samples from normal spleen, skin, liver and colon were analyzed. Cryo-Gel embedded tissues showed good morphological preservation and no interference in immunohistochemical or immunofluorescent investigations. The quality of extracted RNA and DNA was good. The number of proteins identified using MS was similar between Cryo-Gel embedded samples, samples without embedding compound and OCT embedded samples. However, polymers in the OCT disturbed the signal in the MS, while this was not observed in the Cryo-Gel embedded samples. We conclude that embedding of renal biopsies in Cryo-Gel is an excellent and preferable alternative for OCT compound for both diagnostic and research purposes, especially in those cases where proteomic analysis might be necessary.
format article
author Malou L. H. Snijders
Marina Zajec
Laurens A. J. Walter
Remco M. A. A. de Louw
Monique H. A. Oomen
Shazia Arshad
Thierry P. P. van den Bosch
Lennard J. M. Dekker
Michail Doukas
Theo M. Luider
Peter H. J. Riegman
Folkert J. van Kemenade
Marian C. Clahsen-van Groningen
author_facet Malou L. H. Snijders
Marina Zajec
Laurens A. J. Walter
Remco M. A. A. de Louw
Monique H. A. Oomen
Shazia Arshad
Thierry P. P. van den Bosch
Lennard J. M. Dekker
Michail Doukas
Theo M. Luider
Peter H. J. Riegman
Folkert J. van Kemenade
Marian C. Clahsen-van Groningen
author_sort Malou L. H. Snijders
title Cryo-Gel embedding compound for renal biopsy biobanking
title_short Cryo-Gel embedding compound for renal biopsy biobanking
title_full Cryo-Gel embedding compound for renal biopsy biobanking
title_fullStr Cryo-Gel embedding compound for renal biopsy biobanking
title_full_unstemmed Cryo-Gel embedding compound for renal biopsy biobanking
title_sort cryo-gel embedding compound for renal biopsy biobanking
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/0e7d90e3e42941199e6d89072dc636f0
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