The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease
Abstract The objective of this research was to study the effect of Benzothiadiazole (BTH) and Salicylic acid (SA) on the systemic acquired resistance (SAR) of sugarcane the phytoplasma associated with the sugarcane white leaf (SCWL) disease. The experiment was conducted on plants of the sugarcane va...
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2021
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oai:doaj.org-article:0e80a98e07654507a58b179bcbc34c9d2021-12-02T18:47:04ZThe role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease10.1038/s41598-021-94746-92045-2322https://doaj.org/article/0e80a98e07654507a58b179bcbc34c9d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94746-9https://doaj.org/toc/2045-2322Abstract The objective of this research was to study the effect of Benzothiadiazole (BTH) and Salicylic acid (SA) on the systemic acquired resistance (SAR) of sugarcane the phytoplasma associated with the sugarcane white leaf (SCWL) disease. The experiment was conducted on plants of the sugarcane variety Khon Kaen 3 (KK3) infected with SCWL phytoplasma using insect vectors. Biochemical changes related to the SAR such as SA and total phenolic compounds were followed according to 4 different timepoints: 7, 14, 21 and 28 days after inoculation. Together, phytoplasma were quantified by RT-qPCR using the secA gene of phytoplasma. According to our results, the spraying of BTH and SA tended to increase the amounts of SA, total phenolic compounds and a lower presence of phytoplasma in the plants in comparison with the inoculated control. Spraying BTH at a concentration of 2.4 mM and SA at a concentration of 2.4 mM exhibited the best efficiency to reduce the concentration of phytoplasma. According to RT-qPCR results, the inoculated plants sprayed with BTH displayed a significantly lower concentration of phytoplasma compared to the inoculated controls. Overall, our results indicated that the spray of BTH and SA could induce an efficient SAR response to the phytoplasma associated with the SCWL disease. We expect these results will give support to the development of new products for controlling white leaf disease in sugarcane.Manuwat Tintarasara Na RatchaseemaLawan KladsuwanLaurent SoulardPratchya SwangmaneecharernPrapat PunpeePeeraya Klomsa-ardKlanarong SrirothSuttipun KeawsompongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
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Medicine R Science Q Manuwat Tintarasara Na Ratchaseema Lawan Kladsuwan Laurent Soulard Pratchya Swangmaneecharern Prapat Punpee Peeraya Klomsa-ard Klanarong Sriroth Suttipun Keawsompong The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| description |
Abstract The objective of this research was to study the effect of Benzothiadiazole (BTH) and Salicylic acid (SA) on the systemic acquired resistance (SAR) of sugarcane the phytoplasma associated with the sugarcane white leaf (SCWL) disease. The experiment was conducted on plants of the sugarcane variety Khon Kaen 3 (KK3) infected with SCWL phytoplasma using insect vectors. Biochemical changes related to the SAR such as SA and total phenolic compounds were followed according to 4 different timepoints: 7, 14, 21 and 28 days after inoculation. Together, phytoplasma were quantified by RT-qPCR using the secA gene of phytoplasma. According to our results, the spraying of BTH and SA tended to increase the amounts of SA, total phenolic compounds and a lower presence of phytoplasma in the plants in comparison with the inoculated control. Spraying BTH at a concentration of 2.4 mM and SA at a concentration of 2.4 mM exhibited the best efficiency to reduce the concentration of phytoplasma. According to RT-qPCR results, the inoculated plants sprayed with BTH displayed a significantly lower concentration of phytoplasma compared to the inoculated controls. Overall, our results indicated that the spray of BTH and SA could induce an efficient SAR response to the phytoplasma associated with the SCWL disease. We expect these results will give support to the development of new products for controlling white leaf disease in sugarcane. |
| format |
article |
| author |
Manuwat Tintarasara Na Ratchaseema Lawan Kladsuwan Laurent Soulard Pratchya Swangmaneecharern Prapat Punpee Peeraya Klomsa-ard Klanarong Sriroth Suttipun Keawsompong |
| author_facet |
Manuwat Tintarasara Na Ratchaseema Lawan Kladsuwan Laurent Soulard Pratchya Swangmaneecharern Prapat Punpee Peeraya Klomsa-ard Klanarong Sriroth Suttipun Keawsompong |
| author_sort |
Manuwat Tintarasara Na Ratchaseema |
| title |
The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| title_short |
The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| title_full |
The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| title_fullStr |
The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| title_full_unstemmed |
The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| title_sort |
role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/0e80a98e07654507a58b179bcbc34c9d |
| work_keys_str_mv |
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