Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients
Abstract An expansive collateral artery network is correlated with improved survival in case of adverse cardiac episodes. We aimed to identify cellular microRNAs (miRNA; miR) important for collateral artery growth. Chronic total occlusion (CTO) patients (n = 26) were dichotomized using pressure-deri...
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Nature Portfolio
2017
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oai:doaj.org-article:0e85cd1fb88d4b2d94ff98e2213f87702021-12-02T11:40:44ZMonocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients10.1038/s41598-017-01695-32045-2322https://doaj.org/article/0e85cd1fb88d4b2d94ff98e2213f87702017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01695-3https://doaj.org/toc/2045-2322Abstract An expansive collateral artery network is correlated with improved survival in case of adverse cardiac episodes. We aimed to identify cellular microRNAs (miRNA; miR) important for collateral artery growth. Chronic total occlusion (CTO) patients (n = 26) were dichotomized using pressure-derived collateral flow index (CFIp) measurements; high collateral capacity (CFIp > 0.39; n = 14) and low collateral (CFIp < 0.39; n = 12) capacity. MiRNA profiling via next generation sequencing from various monocyte phenotypes (freshly isolated monocytes, monocytes cultured without stimulant, or stimulation with lipopolysaccharide, interleukin 4, transforming growth factor beta-1, or interferon gamma) revealed significantly different miRNA expression patterns between high versus low collateral capacity patients. Validation by real-time polymerase chain reaction demonstrated significantly decreased expression of miR339-5p in all stimulated monocyte phenotypes of low collateral capacity patients. MiR339-5p showed significant correlation with CFIp values in stimulated monocytes. Ingenuity pathway analysis of predicted gene targets of miR339-5p and differential gene expression data from high versus low CFIp patients (n = 20), revealed significant association with STAT3 pathway, and also suggested a possible regulatory role for this signaling pathway. These results identify a novel association between miR339-5p and coronary collateral function. Future work examining modulation of miR339-5p and downstream effects on the STAT3 pathway and subsequent collateral vessel growth are warranted.Nazanin HakimzadehJoëlle EliasGilbert W. M. WijntjensRuud TheunissenAngela van WeertMartijn W. SmuldersNynke van den AkkerPerry D. MoerlandHein J. VerberneLoes P. HoebersJose P. S. HenriquesAnja M. van der LaanMustafa IlhanMark PostSebastiaan C. A. M. BekkersJan J. PiekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
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Medicine R Science Q Nazanin Hakimzadeh Joëlle Elias Gilbert W. M. Wijntjens Ruud Theunissen Angela van Weert Martijn W. Smulders Nynke van den Akker Perry D. Moerland Hein J. Verberne Loes P. Hoebers Jose P. S. Henriques Anja M. van der Laan Mustafa Ilhan Mark Post Sebastiaan C. A. M. Bekkers Jan J. Piek Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients |
| description |
Abstract An expansive collateral artery network is correlated with improved survival in case of adverse cardiac episodes. We aimed to identify cellular microRNAs (miRNA; miR) important for collateral artery growth. Chronic total occlusion (CTO) patients (n = 26) were dichotomized using pressure-derived collateral flow index (CFIp) measurements; high collateral capacity (CFIp > 0.39; n = 14) and low collateral (CFIp < 0.39; n = 12) capacity. MiRNA profiling via next generation sequencing from various monocyte phenotypes (freshly isolated monocytes, monocytes cultured without stimulant, or stimulation with lipopolysaccharide, interleukin 4, transforming growth factor beta-1, or interferon gamma) revealed significantly different miRNA expression patterns between high versus low collateral capacity patients. Validation by real-time polymerase chain reaction demonstrated significantly decreased expression of miR339-5p in all stimulated monocyte phenotypes of low collateral capacity patients. MiR339-5p showed significant correlation with CFIp values in stimulated monocytes. Ingenuity pathway analysis of predicted gene targets of miR339-5p and differential gene expression data from high versus low CFIp patients (n = 20), revealed significant association with STAT3 pathway, and also suggested a possible regulatory role for this signaling pathway. These results identify a novel association between miR339-5p and coronary collateral function. Future work examining modulation of miR339-5p and downstream effects on the STAT3 pathway and subsequent collateral vessel growth are warranted. |
| format |
article |
| author |
Nazanin Hakimzadeh Joëlle Elias Gilbert W. M. Wijntjens Ruud Theunissen Angela van Weert Martijn W. Smulders Nynke van den Akker Perry D. Moerland Hein J. Verberne Loes P. Hoebers Jose P. S. Henriques Anja M. van der Laan Mustafa Ilhan Mark Post Sebastiaan C. A. M. Bekkers Jan J. Piek |
| author_facet |
Nazanin Hakimzadeh Joëlle Elias Gilbert W. M. Wijntjens Ruud Theunissen Angela van Weert Martijn W. Smulders Nynke van den Akker Perry D. Moerland Hein J. Verberne Loes P. Hoebers Jose P. S. Henriques Anja M. van der Laan Mustafa Ilhan Mark Post Sebastiaan C. A. M. Bekkers Jan J. Piek |
| author_sort |
Nazanin Hakimzadeh |
| title |
Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients |
| title_short |
Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients |
| title_full |
Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients |
| title_fullStr |
Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients |
| title_full_unstemmed |
Monocytic microRNA profile associated with coronary collateral artery function in chronic total occlusion patients |
| title_sort |
monocytic microrna profile associated with coronary collateral artery function in chronic total occlusion patients |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/0e85cd1fb88d4b2d94ff98e2213f8770 |
| work_keys_str_mv |
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