Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers

Abstract. Saptadi D, Asbani N, Heliyanto B, Setiawan A, Sudarsono. 2020. Identification of interspecific hybrid between Jatropha curcas x J. integerrima using morphological and molecular markers. Biodiversitas 21: 814-823. Eight F1 progenies derived from Jatropha curcas × J. integerrima hybridizatio...

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Autores principales:	DARMAWAN SAPTADI, NUR ASBANI, BAMBANG HELIYANTO, ASEP SETIAWAN, SUDARSONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	f1 interspecific, microsatellite, molecular marker, morphological variation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0e8bb84b740f409e8aecbf319177ca94
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spelling	oai:doaj.org-article:0e8bb84b740f409e8aecbf319177ca942021-11-21T22:01:10ZIdentification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers1412-033X2085-472210.13057/biodiv/d210249https://doaj.org/article/0e8bb84b740f409e8aecbf319177ca942020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5106https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Saptadi D, Asbani N, Heliyanto B, Setiawan A, Sudarsono. 2020. Identification of interspecific hybrid between Jatropha curcas x J. integerrima using morphological and molecular markers. Biodiversitas 21: 814-823. Eight F1 progenies derived from Jatropha curcas × J. integerrima hybridizations were evaluated for their morphological characters and using RAPD, ISSR and SSR markers. Morphological variations among the hybrids were limited and they were intermediate between the Jatropha parents. The eight F1 progenies derived from J. curcas × J. integerrima hybridizations were most probably the interspecific F1 hybrids. The confirmed identity of the progenies as interspecific hybrids between J. curcas × J. integerrima was based on the presence of several phenotypic characters from both parents in the F1 progenies and by similarity of the molecular marker banding patterns among the parents and the F1 progenies. Among the evaluated molecular markers, the ISSR primers and the majority of either RAPD and SSR primers were not able to generate marker for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. However, the RAPD primer OPC 10 and the SSR primers AF469003, EU099522 and EU586348 were able to generate polymorphic markers in the Jatropha parents and their F1 progenies. Therefore, these four primers were able to generate usable markers for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. The evaluated interspecific F1 progenies are potentially useful to increase genetic diversity of J. curcas and support its breeding program.DARMAWAN SAPTADINUR ASBANIBAMBANG HELIYANTOASEP SETIAWANSUDARSONOMBI & UNS Soloarticlef1 interspecific, microsatellite, molecular marker, morphological variationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	f1 interspecific, microsatellite, molecular marker, morphological variation Biology (General) QH301-705.5
spellingShingle	f1 interspecific, microsatellite, molecular marker, morphological variation Biology (General) QH301-705.5 DARMAWAN SAPTADI NUR ASBANI BAMBANG HELIYANTO ASEP SETIAWAN SUDARSONO Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
description	Abstract. Saptadi D, Asbani N, Heliyanto B, Setiawan A, Sudarsono. 2020. Identification of interspecific hybrid between Jatropha curcas x J. integerrima using morphological and molecular markers. Biodiversitas 21: 814-823. Eight F1 progenies derived from Jatropha curcas × J. integerrima hybridizations were evaluated for their morphological characters and using RAPD, ISSR and SSR markers. Morphological variations among the hybrids were limited and they were intermediate between the Jatropha parents. The eight F1 progenies derived from J. curcas × J. integerrima hybridizations were most probably the interspecific F1 hybrids. The confirmed identity of the progenies as interspecific hybrids between J. curcas × J. integerrima was based on the presence of several phenotypic characters from both parents in the F1 progenies and by similarity of the molecular marker banding patterns among the parents and the F1 progenies. Among the evaluated molecular markers, the ISSR primers and the majority of either RAPD and SSR primers were not able to generate marker for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. However, the RAPD primer OPC 10 and the SSR primers AF469003, EU099522 and EU586348 were able to generate polymorphic markers in the Jatropha parents and their F1 progenies. Therefore, these four primers were able to generate usable markers for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. The evaluated interspecific F1 progenies are potentially useful to increase genetic diversity of J. curcas and support its breeding program.
format	article
author	DARMAWAN SAPTADI NUR ASBANI BAMBANG HELIYANTO ASEP SETIAWAN SUDARSONO
author_facet	DARMAWAN SAPTADI NUR ASBANI BAMBANG HELIYANTO ASEP SETIAWAN SUDARSONO
author_sort	DARMAWAN SAPTADI
title	Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
title_short	Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
title_full	Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
title_fullStr	Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
title_full_unstemmed	Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
title_sort	identification of interspecific hybrid between jatropha curcas × j. integerrima using morphological and molecular markers
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/0e8bb84b740f409e8aecbf319177ca94
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_version_	1718418564548919296

Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers

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