Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate

Abstract In S. aureus biofilms, bacteria are embedded in a matrix of extracellular polymeric substances (EPS) and are highly tolerant to antimicrobial drugs. We thus sought to identify non-antibiotic substances with broad-spectrum activity able to destroy the EPS matrix and enhance the effect of ant...

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Autores principales:	JingJing Liu, Jean-Yves Madec, Alain Bousquet-Mélou, Marisa Haenni, Aude A. Ferran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e8f2adad2b543d2a92fbf12f10b482b
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spelling	oai:doaj.org-article:0e8f2adad2b543d2a92fbf12f10b482b2021-12-02T17:05:11ZDestruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate10.1038/s41598-021-85722-42045-2322https://doaj.org/article/0e8f2adad2b543d2a92fbf12f10b482b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85722-4https://doaj.org/toc/2045-2322Abstract In S. aureus biofilms, bacteria are embedded in a matrix of extracellular polymeric substances (EPS) and are highly tolerant to antimicrobial drugs. We thus sought to identify non-antibiotic substances with broad-spectrum activity able to destroy the EPS matrix and enhance the effect of antibiotics on embedded biofilm bacteria. Among eight substances tested, subtilisin A (0.01 U/mL) and calcium gluconate (CaG, Ca2+ 1.25 mmol/L) significantly reduced the biomass of biofilms formed by at least 21/24 S. aureus isolates. Confocal laser scanning microscopy confirmed that they both eliminated nearly all the proteins and PNAG from the matrix. By contrast, antibiotics alone had nearly no effect on biofilm biomass and the selected one (oxytetracycline-OTC) could only slightly reduce biofilm bacteria. The combination of OTC with CaG or subtilisin A led to an additive reduction (average of 2 log10 CFU/mL) of embedded biofilm bacteria on the isolates susceptible to OTC (MBC < 10 μg/mL, 11/24). Moreover, these two combinations led to a reduction of the embedded biofilm bacteria higher than 3 log10 CFU/mL for 20–25% of the isolates. Further studies are now required to better understand the factors that cause the biofilm produced by specific isolates (20–25%) to be susceptible to the combinations.JingJing LiuJean-Yves MadecAlain Bousquet-MélouMarisa HaenniAude A. FerranNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q JingJing Liu Jean-Yves Madec Alain Bousquet-Mélou Marisa Haenni Aude A. Ferran Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate
description	Abstract In S. aureus biofilms, bacteria are embedded in a matrix of extracellular polymeric substances (EPS) and are highly tolerant to antimicrobial drugs. We thus sought to identify non-antibiotic substances with broad-spectrum activity able to destroy the EPS matrix and enhance the effect of antibiotics on embedded biofilm bacteria. Among eight substances tested, subtilisin A (0.01 U/mL) and calcium gluconate (CaG, Ca2+ 1.25 mmol/L) significantly reduced the biomass of biofilms formed by at least 21/24 S. aureus isolates. Confocal laser scanning microscopy confirmed that they both eliminated nearly all the proteins and PNAG from the matrix. By contrast, antibiotics alone had nearly no effect on biofilm biomass and the selected one (oxytetracycline-OTC) could only slightly reduce biofilm bacteria. The combination of OTC with CaG or subtilisin A led to an additive reduction (average of 2 log10 CFU/mL) of embedded biofilm bacteria on the isolates susceptible to OTC (MBC < 10 μg/mL, 11/24). Moreover, these two combinations led to a reduction of the embedded biofilm bacteria higher than 3 log10 CFU/mL for 20–25% of the isolates. Further studies are now required to better understand the factors that cause the biofilm produced by specific isolates (20–25%) to be susceptible to the combinations.
format	article
author	JingJing Liu Jean-Yves Madec Alain Bousquet-Mélou Marisa Haenni Aude A. Ferran
author_facet	JingJing Liu Jean-Yves Madec Alain Bousquet-Mélou Marisa Haenni Aude A. Ferran
author_sort	JingJing Liu
title	Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate
title_short	Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate
title_full	Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate
title_fullStr	Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate
title_full_unstemmed	Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate
title_sort	destruction of staphylococcus aureus biofilms by combining an antibiotic with subtilisin a or calcium gluconate
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0e8f2adad2b543d2a92fbf12f10b482b
work_keys_str_mv	AT jingjingliu destructionofstaphylococcusaureusbiofilmsbycombininganantibioticwithsubtilisinaorcalciumgluconate AT jeanyvesmadec destructionofstaphylococcusaureusbiofilmsbycombininganantibioticwithsubtilisinaorcalciumgluconate AT alainbousquetmelou destructionofstaphylococcusaureusbiofilmsbycombininganantibioticwithsubtilisinaorcalciumgluconate AT marisahaenni destructionofstaphylococcusaureusbiofilmsbycombininganantibioticwithsubtilisinaorcalciumgluconate AT audeaferran destructionofstaphylococcusaureusbiofilmsbycombininganantibioticwithsubtilisinaorcalciumgluconate
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Destruction of Staphylococcus aureus biofilms by combining an antibiotic with subtilisin A or calcium gluconate

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