Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids

Abstract Membrane fluidity, essential for cell functions, is obviously affected by copper, but the molecular mechanism is poorly understood. Here, we unexpectedly observed that a decrease in phospholipid (PL) bilayer fluidity caused by Cu2+ was more significant than those by Zn2+ and Ca2+, while a c...

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Autores principales:	Xiankai Jiang, Jinjin Zhang, Bo Zhou, Pei Li, Xiaojuan Hu, Zhi Zhu, Yanwen Tan, Chao Chang, Junhong Lü, Bo Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Supported Lipid Bilayers Small Unilamellar Vesicles Suspension Wiberg Bond Order FRAP Experiments Fluorescence Recovery After Photobleaching (FRAP) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e9900c342eb4c9c838c199ccb99afbb
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spelling	oai:doaj.org-article:0e9900c342eb4c9c838c199ccb99afbb2021-12-02T15:08:55ZAnomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids10.1038/s41598-018-32322-42045-2322https://doaj.org/article/0e9900c342eb4c9c838c199ccb99afbb2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32322-4https://doaj.org/toc/2045-2322Abstract Membrane fluidity, essential for cell functions, is obviously affected by copper, but the molecular mechanism is poorly understood. Here, we unexpectedly observed that a decrease in phospholipid (PL) bilayer fluidity caused by Cu2+ was more significant than those by Zn2+ and Ca2+, while a comparable reduction occurred in the last two ions. This finding disagrees with the placement in the periodic table of Cu just next to Zn and far from Ca. The physical nature was revealed to be an anomalous attraction between Cu+ cations, as well as the induced motif of two phospholipids coupled by Cu-Cu bond (PL-diCu-PL). Namely, upon Cu2+ ion binding to a negatively charged phosphate group of lipid, Cu2+ was reduced to Cu+. The attraction of the cations then caused one Cu+ ion simultaneously binding to two lipids and another Cu+, resulting in the formation of PL-diCu-PL structure. In contrast, this attraction cannot occur in the cases of Zn and Ca ions. Remarkably, besides lipids, the phosphate group also widely exists in other biological molecules, including DNA, RNA, ADP and ATP. Our findings thus provide a new view for understanding the biological functions of copper and the mechanism underlying copper-related diseases, as well as lipid assembly.Xiankai JiangJinjin ZhangBo ZhouPei LiXiaojuan HuZhi ZhuYanwen TanChao ChangJunhong LüBo SongNature PortfolioarticleSupported Lipid BilayersSmall Unilamellar Vesicles SuspensionWiberg Bond OrderFRAP ExperimentsFluorescence Recovery After Photobleaching (FRAP)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Supported Lipid Bilayers Small Unilamellar Vesicles Suspension Wiberg Bond Order FRAP Experiments Fluorescence Recovery After Photobleaching (FRAP) Medicine R Science Q
spellingShingle	Supported Lipid Bilayers Small Unilamellar Vesicles Suspension Wiberg Bond Order FRAP Experiments Fluorescence Recovery After Photobleaching (FRAP) Medicine R Science Q Xiankai Jiang Jinjin Zhang Bo Zhou Pei Li Xiaojuan Hu Zhi Zhu Yanwen Tan Chao Chang Junhong Lü Bo Song Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
description	Abstract Membrane fluidity, essential for cell functions, is obviously affected by copper, but the molecular mechanism is poorly understood. Here, we unexpectedly observed that a decrease in phospholipid (PL) bilayer fluidity caused by Cu2+ was more significant than those by Zn2+ and Ca2+, while a comparable reduction occurred in the last two ions. This finding disagrees with the placement in the periodic table of Cu just next to Zn and far from Ca. The physical nature was revealed to be an anomalous attraction between Cu+ cations, as well as the induced motif of two phospholipids coupled by Cu-Cu bond (PL-diCu-PL). Namely, upon Cu2+ ion binding to a negatively charged phosphate group of lipid, Cu2+ was reduced to Cu+. The attraction of the cations then caused one Cu+ ion simultaneously binding to two lipids and another Cu+, resulting in the formation of PL-diCu-PL structure. In contrast, this attraction cannot occur in the cases of Zn and Ca ions. Remarkably, besides lipids, the phosphate group also widely exists in other biological molecules, including DNA, RNA, ADP and ATP. Our findings thus provide a new view for understanding the biological functions of copper and the mechanism underlying copper-related diseases, as well as lipid assembly.
format	article
author	Xiankai Jiang Jinjin Zhang Bo Zhou Pei Li Xiaojuan Hu Zhi Zhu Yanwen Tan Chao Chang Junhong Lü Bo Song
author_facet	Xiankai Jiang Jinjin Zhang Bo Zhou Pei Li Xiaojuan Hu Zhi Zhu Yanwen Tan Chao Chang Junhong Lü Bo Song
author_sort	Xiankai Jiang
title	Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
title_short	Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
title_full	Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
title_fullStr	Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
title_full_unstemmed	Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
title_sort	anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0e9900c342eb4c9c838c199ccb99afbb
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Anomalous behavior of membrane fluidity caused by copper-copper bond coupled phospholipids

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