ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA

Objective: To determine the frequency of Del 13q14 in Chronic lymphocytic leukaemia, to compare its association with clinicpathologic features and to define the contribution of this abnormality to the prognosis. Study Design: Cross-sectional study. Place and Duration of Study: Department of Ha...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ali Ahmed, Ch Altaf Hussain, Mohammad Abdul Naeem, Hamid Saeed Malik, Maria Khan, Saqib Hussain Korejo
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2021
Materias:	chronic lymphocytic leukaemia fluorescence in situ hybridization del 13q14 trisomy 12 Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0ea462e6b62c4120ab8c952dc6099058
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0ea462e6b62c4120ab8c952dc6099058
record_format	dspace
spelling	oai:doaj.org-article:0ea462e6b62c4120ab8c952dc60990582021-12-02T18:44:47ZASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA0030-96482411-8842https://doaj.org/article/0ea462e6b62c4120ab8c952dc60990582021-04-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/6671https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the frequency of Del 13q14 in Chronic lymphocytic leukaemia, to compare its association with clinicpathologic features and to define the contribution of this abnormality to the prognosis. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Armed Forces Bone Marrow Transplant Centre and Oncology department, Combined Military Hospital Rawalpindi, from Apr 2017 to Jul 2018. Methodology: A total of 56 newly diagnosed cases of CLL were included in the study. Patients were diagnosed on the basis of National Cancer Institute Working Group guidelines for diagnosis of CLL. After detailed history and thorough clinical examination; complete blood counts, biochemical profile, bone marrow examination, immunophenotyping on bone marrow or peripheral blood samples were done and Interphase FISH studies on blood or bone marrow specimens for detection of Del 13q14 were performed. Clinico-pathological features of CLL patients with Del 13q14 were compared with other cytogenetic abnormalities. Results: The frequency of Trisomy 12 was found to be 37.5%. Most of CLL patients with Del 13q14 were aymptomatic and were diagnosed on routine workup. The WBC count and Absolute lymphocyte count was slightly lower in patients with Del13 q14 lower when compared with the CLL patients without 13q14 Deletion. Most of the patients with this aberration presented in early stage (Binet stage A) and this association of Del 13q14 with Binet stage was statistically significant (p<0.05). However, no association was found between 13q14 deletion and ZAP70 as all of our patients were negative for this marker. Many patients with Del 13q14 did not require chemotherapy at diagnosis and during follow up as compared to patients without del13q14, and this association was statistically significant (p<0.05). The study also showed that disease progression in patients with deletion 13q14 become significantly less as compared to patient without Del 13q14. Conclusions: The deletion of 13q14 influence clinical outcome of patients with CLL and was found to be associated with favourable prognosis. A determination of Del 13q14 should therefore be included in the investigations of the prognostic factors of B-cell chronic lymphocytic leukemia. This can prevent unwanted chemotherapy in these patients.Ali AhmedCh Altaf HussainMohammad Abdul NaeemHamid Saeed MalikMaria KhanSaqib Hussain KorejoArmy Medical College Rawalpindiarticlechronic lymphocytic leukaemiafluorescence in situ hybridizationdel 13q14trisomy 12MedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 71, Iss 2, Pp 579-83 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic lymphocytic leukaemia fluorescence in situ hybridization del 13q14 trisomy 12 Medicine R Medicine (General) R5-920
spellingShingle	chronic lymphocytic leukaemia fluorescence in situ hybridization del 13q14 trisomy 12 Medicine R Medicine (General) R5-920 Ali Ahmed Ch Altaf Hussain Mohammad Abdul Naeem Hamid Saeed Malik Maria Khan Saqib Hussain Korejo ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA
description	Objective: To determine the frequency of Del 13q14 in Chronic lymphocytic leukaemia, to compare its association with clinicpathologic features and to define the contribution of this abnormality to the prognosis. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Armed Forces Bone Marrow Transplant Centre and Oncology department, Combined Military Hospital Rawalpindi, from Apr 2017 to Jul 2018. Methodology: A total of 56 newly diagnosed cases of CLL were included in the study. Patients were diagnosed on the basis of National Cancer Institute Working Group guidelines for diagnosis of CLL. After detailed history and thorough clinical examination; complete blood counts, biochemical profile, bone marrow examination, immunophenotyping on bone marrow or peripheral blood samples were done and Interphase FISH studies on blood or bone marrow specimens for detection of Del 13q14 were performed. Clinico-pathological features of CLL patients with Del 13q14 were compared with other cytogenetic abnormalities. Results: The frequency of Trisomy 12 was found to be 37.5%. Most of CLL patients with Del 13q14 were aymptomatic and were diagnosed on routine workup. The WBC count and Absolute lymphocyte count was slightly lower in patients with Del13 q14 lower when compared with the CLL patients without 13q14 Deletion. Most of the patients with this aberration presented in early stage (Binet stage A) and this association of Del 13q14 with Binet stage was statistically significant (p<0.05). However, no association was found between 13q14 deletion and ZAP70 as all of our patients were negative for this marker. Many patients with Del 13q14 did not require chemotherapy at diagnosis and during follow up as compared to patients without del13q14, and this association was statistically significant (p<0.05). The study also showed that disease progression in patients with deletion 13q14 become significantly less as compared to patient without Del 13q14. Conclusions: The deletion of 13q14 influence clinical outcome of patients with CLL and was found to be associated with favourable prognosis. A determination of Del 13q14 should therefore be included in the investigations of the prognostic factors of B-cell chronic lymphocytic leukemia. This can prevent unwanted chemotherapy in these patients.
format	article
author	Ali Ahmed Ch Altaf Hussain Mohammad Abdul Naeem Hamid Saeed Malik Maria Khan Saqib Hussain Korejo
author_facet	Ali Ahmed Ch Altaf Hussain Mohammad Abdul Naeem Hamid Saeed Malik Maria Khan Saqib Hussain Korejo
author_sort	Ali Ahmed
title	ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA
title_short	ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA
title_full	ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA
title_fullStr	ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA
title_full_unstemmed	ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA
title_sort	association of deletion 13q14 with clinicopathologic features in chronic lymphocytic leukemia
publisher	Army Medical College Rawalpindi
publishDate	2021
url	https://doaj.org/article/0ea462e6b62c4120ab8c952dc6099058
work_keys_str_mv	AT aliahmed associationofdeletion13q14withclinicopathologicfeaturesinchroniclymphocyticleukemia AT chaltafhussain associationofdeletion13q14withclinicopathologicfeaturesinchroniclymphocyticleukemia AT mohammadabdulnaeem associationofdeletion13q14withclinicopathologicfeaturesinchroniclymphocyticleukemia AT hamidsaeedmalik associationofdeletion13q14withclinicopathologicfeaturesinchroniclymphocyticleukemia AT mariakhan associationofdeletion13q14withclinicopathologicfeaturesinchroniclymphocyticleukemia AT saqibhussainkorejo associationofdeletion13q14withclinicopathologicfeaturesinchroniclymphocyticleukemia
_version_	1718377663340478464

ASSOCIATION OF DELETION 13Q14 WITH CLINICOPATHOLOGIC FEATURES IN CHRONIC LYMPHOCYTIC LEUKEMIA

Ejemplares similares