From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.

<h4>Objective</h4>To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in...

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Autores principales:	Lesla E S Bruijnesteijn van Coppenraet, Jacky Flipse, Janny A Wallinga, Marloes Vermeer, Wil A van der Reijden, Jan F L Weel, Adri G M van der Zanden, Theo A Schuurs, Gijs J H M Ruijs
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0eac3db073844807829795735a2953fc
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spelling	oai:doaj.org-article:0eac3db073844807829795735a2953fc2021-12-02T20:04:29ZFrom a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.1932-620310.1371/journal.pone.0258680https://doaj.org/article/0eac3db073844807829795735a2953fc2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258680https://doaj.org/toc/1932-6203<h4>Objective</h4>To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in the Netherlands (2010-2012).<h4>Methods</h4>Archived stool samples from 1340 cases and 1100 controls were retested using internally controlled multiplex real-time PCRs for putative pathogenic gastroenteritis viruses: adenovirus, astrovirus, bocavirus, enterovirus, norovirus GI and GII, human parechovirus, rotavirus, salivirus, sapovirus, and torovirus.<h4>Results</h4>The prevalence of any virus in symptomatic cases and asymptomatic controls was 16.6% (223/1340) and 10.2% (112/1100), respectively. Prevalence of astrovirus (adjusted odds ratio (aOR) 10.37; 95% confidence interval (CI) 1.34-80.06) and norovirus GII (aOR 3.10; CI 1.62-5.92) was significantly higher in cases versus controls. Rotavirus was encountered only in cases. We did not find torovirus and there was no statistically significant relationship with cases for salivirus (aOR 1,67; (CI) 0.43-6.54)), adenovirus non-group F (aOR 1.20; CI 0.75-1.91), bocavirus (aOR 0.85; CI 0.05-13.64), enterovirus (aOR 0.83; CI 0.50-1.37), human parechovirus (aOR 1.61; CI 0.54-4.77) and sapovirus (aOR 1.15; CI 0.67-1.98). Though adenovirus group F (aOR 6.37; CI 0.80-50.92) and norovirus GI (aOR 2.22, CI: 0.79-6.23) are known enteropathogenic viruses and were more prevalent in cases than in controls, this did not reach significance in this study. The Ct value did not discriminate between carriage and disease in PCR-positive subjects.<h4>Conclusions</h4>In our population, diagnostic gastroenteritis tests should screen for adenovirus group F, astrovirus, noroviruses GI and GII, and rotavirus. Case-control studies as ours are lacking and should also be carried out in populations from other epidemiological backgrounds.Lesla E S Bruijnesteijn van CoppenraetJacky FlipseJanny A WallingaMarloes VermeerWil A van der ReijdenJan F L WeelAdri G M van der ZandenTheo A SchuursGijs J H M RuijsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0258680 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lesla E S Bruijnesteijn van Coppenraet Jacky Flipse Janny A Wallinga Marloes Vermeer Wil A van der Reijden Jan F L Weel Adri G M van der Zanden Theo A Schuurs Gijs J H M Ruijs From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
description	<h4>Objective</h4>To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in the Netherlands (2010-2012).<h4>Methods</h4>Archived stool samples from 1340 cases and 1100 controls were retested using internally controlled multiplex real-time PCRs for putative pathogenic gastroenteritis viruses: adenovirus, astrovirus, bocavirus, enterovirus, norovirus GI and GII, human parechovirus, rotavirus, salivirus, sapovirus, and torovirus.<h4>Results</h4>The prevalence of any virus in symptomatic cases and asymptomatic controls was 16.6% (223/1340) and 10.2% (112/1100), respectively. Prevalence of astrovirus (adjusted odds ratio (aOR) 10.37; 95% confidence interval (CI) 1.34-80.06) and norovirus GII (aOR 3.10; CI 1.62-5.92) was significantly higher in cases versus controls. Rotavirus was encountered only in cases. We did not find torovirus and there was no statistically significant relationship with cases for salivirus (aOR 1,67; (CI) 0.43-6.54)), adenovirus non-group F (aOR 1.20; CI 0.75-1.91), bocavirus (aOR 0.85; CI 0.05-13.64), enterovirus (aOR 0.83; CI 0.50-1.37), human parechovirus (aOR 1.61; CI 0.54-4.77) and sapovirus (aOR 1.15; CI 0.67-1.98). Though adenovirus group F (aOR 6.37; CI 0.80-50.92) and norovirus GI (aOR 2.22, CI: 0.79-6.23) are known enteropathogenic viruses and were more prevalent in cases than in controls, this did not reach significance in this study. The Ct value did not discriminate between carriage and disease in PCR-positive subjects.<h4>Conclusions</h4>In our population, diagnostic gastroenteritis tests should screen for adenovirus group F, astrovirus, noroviruses GI and GII, and rotavirus. Case-control studies as ours are lacking and should also be carried out in populations from other epidemiological backgrounds.
format	article
author	Lesla E S Bruijnesteijn van Coppenraet Jacky Flipse Janny A Wallinga Marloes Vermeer Wil A van der Reijden Jan F L Weel Adri G M van der Zanden Theo A Schuurs Gijs J H M Ruijs
author_facet	Lesla E S Bruijnesteijn van Coppenraet Jacky Flipse Janny A Wallinga Marloes Vermeer Wil A van der Reijden Jan F L Weel Adri G M van der Zanden Theo A Schuurs Gijs J H M Ruijs
author_sort	Lesla E S Bruijnesteijn van Coppenraet
title	From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
title_short	From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
title_full	From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
title_fullStr	From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
title_full_unstemmed	From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
title_sort	from a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0eac3db073844807829795735a2953fc
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From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners' patients.

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