Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.

We have investigated the underlying mechanism by which direct cell-cell contact enhances the efficiency of cell-to-cell transmission of retroviruses. Applying 4D imaging to a model retrovirus, the murine leukemia virus, we directly monitor and quantify sequential assembly, release, and transmission...

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Autores principales:	Jing Jin, Nathan M Sherer, Gisela Heidecker, David Derse, Walther Mothes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0eae1a6ed15f474a8c410f2c61120e91
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spelling	oai:doaj.org-article:0eae1a6ed15f474a8c410f2c61120e912021-11-25T05:34:05ZAssembly of the murine leukemia virus is directed towards sites of cell-cell contact.1544-91731545-788510.1371/journal.pbio.1000163https://doaj.org/article/0eae1a6ed15f474a8c410f2c61120e912009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19636361/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885We have investigated the underlying mechanism by which direct cell-cell contact enhances the efficiency of cell-to-cell transmission of retroviruses. Applying 4D imaging to a model retrovirus, the murine leukemia virus, we directly monitor and quantify sequential assembly, release, and transmission events for individual viral particles as they happen in living cells. We demonstrate that de novo assembly is highly polarized towards zones of cell-cell contact. Viruses assembled approximately 10-fold more frequently at zones of cell contact with no change in assembly kinetics. Gag proteins were drawn to adhesive zones formed by viral Env glycoprotein and its cognate receptor to promote virus assembly at cell-cell contact. This process was dependent on the cytoplasmic tail of viral Env. Env lacking the cytoplasmic tail while still allowing for contact formation, failed to direct virus assembly towards contact sites. Our data describe a novel role for the viral Env glycoprotein in establishing cell-cell adhesion and polarization of assembly prior to becoming a fusion protein to allow virus entry into cells.Jing JinNathan M ShererGisela HeideckerDavid DerseWalther MothesPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 7, p e1000163 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jing Jin Nathan M Sherer Gisela Heidecker David Derse Walther Mothes Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
description	We have investigated the underlying mechanism by which direct cell-cell contact enhances the efficiency of cell-to-cell transmission of retroviruses. Applying 4D imaging to a model retrovirus, the murine leukemia virus, we directly monitor and quantify sequential assembly, release, and transmission events for individual viral particles as they happen in living cells. We demonstrate that de novo assembly is highly polarized towards zones of cell-cell contact. Viruses assembled approximately 10-fold more frequently at zones of cell contact with no change in assembly kinetics. Gag proteins were drawn to adhesive zones formed by viral Env glycoprotein and its cognate receptor to promote virus assembly at cell-cell contact. This process was dependent on the cytoplasmic tail of viral Env. Env lacking the cytoplasmic tail while still allowing for contact formation, failed to direct virus assembly towards contact sites. Our data describe a novel role for the viral Env glycoprotein in establishing cell-cell adhesion and polarization of assembly prior to becoming a fusion protein to allow virus entry into cells.
format	article
author	Jing Jin Nathan M Sherer Gisela Heidecker David Derse Walther Mothes
author_facet	Jing Jin Nathan M Sherer Gisela Heidecker David Derse Walther Mothes
author_sort	Jing Jin
title	Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
title_short	Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
title_full	Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
title_fullStr	Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
title_full_unstemmed	Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
title_sort	assembly of the murine leukemia virus is directed towards sites of cell-cell contact.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/0eae1a6ed15f474a8c410f2c61120e91
work_keys_str_mv	AT jingjin assemblyofthemurineleukemiavirusisdirectedtowardssitesofcellcellcontact AT nathanmsherer assemblyofthemurineleukemiavirusisdirectedtowardssitesofcellcellcontact AT giselaheidecker assemblyofthemurineleukemiavirusisdirectedtowardssitesofcellcellcontact AT davidderse assemblyofthemurineleukemiavirusisdirectedtowardssitesofcellcellcontact AT walthermothes assemblyofthemurineleukemiavirusisdirectedtowardssitesofcellcellcontact
_version_	1718414560009912320

Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.

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