Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia

Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Method...

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Autores principales:	Concepción Santiago-Fernández, Flores Martín-Reyes, Monica Tome, Carolina Gutierrez-Repiso, Diego Fernandez-Garcia, Luis Ocaña-Wilhelmi, Jose Rivas-Becerra, Franz Tatzber, Edith Pursch, Francisco J. Tinahones, Eduardo García-Fuentes, Lourdes Garrido-Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hypoxia scavenger receptors oxidized LDL adipocytes morbid obesity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0eaeff0169834bdca0344bfcaeb08455
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spelling	oai:doaj.org-article:0eaeff0169834bdca0344bfcaeb084552021-11-25T16:51:12ZOxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia10.3390/biomedicines91117152227-9059https://doaj.org/article/0eaeff0169834bdca0344bfcaeb084552021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1715https://doaj.org/toc/2227-9059Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Methods: We studied in 17 non-obese and 20 subjects with morbid obesity (MO) the mRNA expression of HIF-1α, SRs (LOX-1, MSR1, CL-P1 and CXCL16), IL6 and TNFα in visceral adipocytes and the effect of hypoxia with or without ox-LDL on visceral in vitro-differentiated adipocytes (VDA). Results: HIF-1α, TNFα, IL6, LOX-1, MSR1 and CXCL16 expression in adipocytes was increased in MO when compared with those in non-obese subjects (<i>p</i> < 0.05). The expression of most of the inflammatory markers and SRs gene correlated with HIF-1α. In VDA, hypoxia increased TNFα, IL6, MSR1, CXCL16 and CL-P1 (<i>p</i> < 0.05) in non-obese subjects, and TNFα, IL6, MSR1 and CXCL16 (<i>p</i> < 0.05) in MO. Silencing HIF-1α prevented the increase of TNFα, IL6, LOX-1, MSR1, CL-P1 and CXCL16 expression (<i>p</i> < 0.05). The combination of hypoxia and ox-LDL produced higher TNFα expression (<i>p</i> = 0.041). Conclusions: Morbid obesity and hypoxia increased SRs and inflammatory markers in visceral adipocytes. In a hypoxic state, ox-LDL increased the proinflammatory response of visceral adipocytes to hypoxia.Concepción Santiago-FernándezFlores Martín-ReyesMonica TomeCarolina Gutierrez-RepisoDiego Fernandez-GarciaLuis Ocaña-WilhelmiJose Rivas-BecerraFranz TatzberEdith PurschFrancisco J. TinahonesEduardo García-FuentesLourdes Garrido-SánchezMDPI AGarticlehypoxiascavenger receptorsoxidized LDLadipocytesmorbid obesityBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1715, p 1715 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypoxia scavenger receptors oxidized LDL adipocytes morbid obesity Biology (General) QH301-705.5
spellingShingle	hypoxia scavenger receptors oxidized LDL adipocytes morbid obesity Biology (General) QH301-705.5 Concepción Santiago-Fernández Flores Martín-Reyes Monica Tome Carolina Gutierrez-Repiso Diego Fernandez-Garcia Luis Ocaña-Wilhelmi Jose Rivas-Becerra Franz Tatzber Edith Pursch Francisco J. Tinahones Eduardo García-Fuentes Lourdes Garrido-Sánchez Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
description	Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Methods: We studied in 17 non-obese and 20 subjects with morbid obesity (MO) the mRNA expression of HIF-1α, SRs (LOX-1, MSR1, CL-P1 and CXCL16), IL6 and TNFα in visceral adipocytes and the effect of hypoxia with or without ox-LDL on visceral in vitro-differentiated adipocytes (VDA). Results: HIF-1α, TNFα, IL6, LOX-1, MSR1 and CXCL16 expression in adipocytes was increased in MO when compared with those in non-obese subjects (<i>p</i> < 0.05). The expression of most of the inflammatory markers and SRs gene correlated with HIF-1α. In VDA, hypoxia increased TNFα, IL6, MSR1, CXCL16 and CL-P1 (<i>p</i> < 0.05) in non-obese subjects, and TNFα, IL6, MSR1 and CXCL16 (<i>p</i> < 0.05) in MO. Silencing HIF-1α prevented the increase of TNFα, IL6, LOX-1, MSR1, CL-P1 and CXCL16 expression (<i>p</i> < 0.05). The combination of hypoxia and ox-LDL produced higher TNFα expression (<i>p</i> = 0.041). Conclusions: Morbid obesity and hypoxia increased SRs and inflammatory markers in visceral adipocytes. In a hypoxic state, ox-LDL increased the proinflammatory response of visceral adipocytes to hypoxia.
format	article
author	Concepción Santiago-Fernández Flores Martín-Reyes Monica Tome Carolina Gutierrez-Repiso Diego Fernandez-Garcia Luis Ocaña-Wilhelmi Jose Rivas-Becerra Franz Tatzber Edith Pursch Francisco J. Tinahones Eduardo García-Fuentes Lourdes Garrido-Sánchez
author_facet	Concepción Santiago-Fernández Flores Martín-Reyes Monica Tome Carolina Gutierrez-Repiso Diego Fernandez-Garcia Luis Ocaña-Wilhelmi Jose Rivas-Becerra Franz Tatzber Edith Pursch Francisco J. Tinahones Eduardo García-Fuentes Lourdes Garrido-Sánchez
author_sort	Concepción Santiago-Fernández
title	Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
title_short	Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
title_full	Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
title_fullStr	Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
title_full_unstemmed	Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
title_sort	oxidized ldl increase the proinflammatory profile of human visceral adipocytes produced by hypoxia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0eaeff0169834bdca0344bfcaeb08455
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Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia

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