A novel piggyBac transposon inducible expression system identifies a role for AKT signalling in primordial germ cell migration.
In this work, we describe a single piggyBac transposon system containing both a tet-activator and a doxycycline-inducible expression cassette. We demonstrate that a gene product can be conditionally expressed from the integrated transposon and a second gene can be simultaneously targeted by a short...
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Autores principales: | James D Glover, Lorna Taylor, Adrian Sherman, Caroline Zeiger-Poli, Helen M Sang, Michael J McGrew |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2013
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Materias: | |
Acceso en línea: | https://doaj.org/article/0eb05673365341ecaf68a58a5c7dffb9 |
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