Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet

Abstract In order to identify aqueous species formed in Plasma activated media (PAM), quantitative investigations of reactive oxygen and nitrogen species (ROS, RNS) were performed and compared to Milli-Q water and culture media without and with Fetal Calf Serum. Electron paramagnetic resonance, fluo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julie Chauvin, Florian Judée, Mohammed Yousfi, Patricia Vicendo, Nofel Merbahi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0eb1aa4aca2c4439a22ca0dfa5047b66
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0eb1aa4aca2c4439a22ca0dfa5047b66
record_format	dspace
spelling	oai:doaj.org-article:0eb1aa4aca2c4439a22ca0dfa5047b662021-12-02T12:32:06ZAnalysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet10.1038/s41598-017-04650-42045-2322https://doaj.org/article/0eb1aa4aca2c4439a22ca0dfa5047b662017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04650-4https://doaj.org/toc/2045-2322Abstract In order to identify aqueous species formed in Plasma activated media (PAM), quantitative investigations of reactive oxygen and nitrogen species (ROS, RNS) were performed and compared to Milli-Q water and culture media without and with Fetal Calf Serum. Electron paramagnetic resonance, fluorometric and colorimetric analysis were used to identify and quantify free radicals generated by helium plasma jet in these liquids. Results clearly show the formation of ROS such as hydroxyl radical, superoxide anion radical and singlet oxygen in order of the micromolar range of concentrations. Nitric oxide, hydrogen peroxide and nitrite-nitrate anions (in range of several hundred micromolars) are the major species observed in PAM. The composition of the medium has a major impact on the pH of the solution during plasma treatment, on the stability of the different RONS that are produced and on their reactivity with biomolecules. To emphasize the interactions of plasma with a complex medium, amino acid degradation by means of mass spectrometry was also investigated using methionine, tyrosine, tryptophan and arginine. All of these components such as long lifetime RONS and oxidized biological compounds may contribute to the cytotoxic effect of PAM. This study provides mechanistic insights into the mechanisms involved in cell death after treatment with PAM.Julie ChauvinFlorian JudéeMohammed YousfiPatricia VicendoNofel MerbahiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julie Chauvin Florian Judée Mohammed Yousfi Patricia Vicendo Nofel Merbahi Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
description	Abstract In order to identify aqueous species formed in Plasma activated media (PAM), quantitative investigations of reactive oxygen and nitrogen species (ROS, RNS) were performed and compared to Milli-Q water and culture media without and with Fetal Calf Serum. Electron paramagnetic resonance, fluorometric and colorimetric analysis were used to identify and quantify free radicals generated by helium plasma jet in these liquids. Results clearly show the formation of ROS such as hydroxyl radical, superoxide anion radical and singlet oxygen in order of the micromolar range of concentrations. Nitric oxide, hydrogen peroxide and nitrite-nitrate anions (in range of several hundred micromolars) are the major species observed in PAM. The composition of the medium has a major impact on the pH of the solution during plasma treatment, on the stability of the different RONS that are produced and on their reactivity with biomolecules. To emphasize the interactions of plasma with a complex medium, amino acid degradation by means of mass spectrometry was also investigated using methionine, tyrosine, tryptophan and arginine. All of these components such as long lifetime RONS and oxidized biological compounds may contribute to the cytotoxic effect of PAM. This study provides mechanistic insights into the mechanisms involved in cell death after treatment with PAM.
format	article
author	Julie Chauvin Florian Judée Mohammed Yousfi Patricia Vicendo Nofel Merbahi
author_facet	Julie Chauvin Florian Judée Mohammed Yousfi Patricia Vicendo Nofel Merbahi
author_sort	Julie Chauvin
title	Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
title_short	Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
title_full	Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
title_fullStr	Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
title_full_unstemmed	Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
title_sort	analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0eb1aa4aca2c4439a22ca0dfa5047b66
work_keys_str_mv	AT juliechauvin analysisofreactiveoxygenandnitrogenspeciesgeneratedinthreeliquidmediabylowtemperatureheliumplasmajet AT florianjudee analysisofreactiveoxygenandnitrogenspeciesgeneratedinthreeliquidmediabylowtemperatureheliumplasmajet AT mohammedyousfi analysisofreactiveoxygenandnitrogenspeciesgeneratedinthreeliquidmediabylowtemperatureheliumplasmajet AT patriciavicendo analysisofreactiveoxygenandnitrogenspeciesgeneratedinthreeliquidmediabylowtemperatureheliumplasmajet AT nofelmerbahi analysisofreactiveoxygenandnitrogenspeciesgeneratedinthreeliquidmediabylowtemperatureheliumplasmajet
_version_	1718394139830124544

Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet

Ejemplares similares