Light microscopy based approach for mapping connectivity with molecular specificity

Mapping neuroanatomy is a foundational goal of connectomics, and the gold standard method is electron microscopy as light microscopy lacks nanoscale resolution. Here the authors develop a strategy using multicolor genetic labeling (Brainbow) and expansion microscopy to map putative synaptic connecti...

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Autores principales:	Fred Y. Shen, Margaret M. Harrington, Logan A. Walker, Hon Pong Jimmy Cheng, Edward S. Boyden, Dawen Cai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0eb3bbd03f284394bd5506dd6b183bad
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spelling	oai:doaj.org-article:0eb3bbd03f284394bd5506dd6b183bad2021-12-02T18:02:16ZLight microscopy based approach for mapping connectivity with molecular specificity10.1038/s41467-020-18422-82041-1723https://doaj.org/article/0eb3bbd03f284394bd5506dd6b183bad2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18422-8https://doaj.org/toc/2041-1723Mapping neuroanatomy is a foundational goal of connectomics, and the gold standard method is electron microscopy as light microscopy lacks nanoscale resolution. Here the authors develop a strategy using multicolor genetic labeling (Brainbow) and expansion microscopy to map putative synaptic connections using light microscopy.Fred Y. ShenMargaret M. HarringtonLogan A. WalkerHon Pong Jimmy ChengEdward S. BoydenDawen CaiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fred Y. Shen Margaret M. Harrington Logan A. Walker Hon Pong Jimmy Cheng Edward S. Boyden Dawen Cai Light microscopy based approach for mapping connectivity with molecular specificity
description	Mapping neuroanatomy is a foundational goal of connectomics, and the gold standard method is electron microscopy as light microscopy lacks nanoscale resolution. Here the authors develop a strategy using multicolor genetic labeling (Brainbow) and expansion microscopy to map putative synaptic connections using light microscopy.
format	article
author	Fred Y. Shen Margaret M. Harrington Logan A. Walker Hon Pong Jimmy Cheng Edward S. Boyden Dawen Cai
author_facet	Fred Y. Shen Margaret M. Harrington Logan A. Walker Hon Pong Jimmy Cheng Edward S. Boyden Dawen Cai
author_sort	Fred Y. Shen
title	Light microscopy based approach for mapping connectivity with molecular specificity
title_short	Light microscopy based approach for mapping connectivity with molecular specificity
title_full	Light microscopy based approach for mapping connectivity with molecular specificity
title_fullStr	Light microscopy based approach for mapping connectivity with molecular specificity
title_full_unstemmed	Light microscopy based approach for mapping connectivity with molecular specificity
title_sort	light microscopy based approach for mapping connectivity with molecular specificity
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0eb3bbd03f284394bd5506dd6b183bad
work_keys_str_mv	AT fredyshen lightmicroscopybasedapproachformappingconnectivitywithmolecularspecificity AT margaretmharrington lightmicroscopybasedapproachformappingconnectivitywithmolecularspecificity AT loganawalker lightmicroscopybasedapproachformappingconnectivitywithmolecularspecificity AT honpongjimmycheng lightmicroscopybasedapproachformappingconnectivitywithmolecularspecificity AT edwardsboyden lightmicroscopybasedapproachformappingconnectivitywithmolecularspecificity AT dawencai lightmicroscopybasedapproachformappingconnectivitywithmolecularspecificity
_version_	1718378910815617024

Light microscopy based approach for mapping connectivity with molecular specificity

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