Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway

Objective The aim of present report was to elucidate the effect of cell division cycle associated 4 (CDCA4) on the proliferation and apoptosis of Wilm’s tumor cells, and to further evaluate its underlying mechanism. Methods The expression profiles of CDCA4 and clinical information of Wilm’s tumor pa...

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Autores principales:	Suqing Li, Cong Qin, Yike Chen, Dan Wei, Zhijun Tan, Jiadong Meng
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cdca4 nephroblastoma proliferation apoptosis Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/0eb8b3087b41420098b4534191fa9c33
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spelling	oai:doaj.org-article:0eb8b3087b41420098b4534191fa9c332021-11-04T15:00:41ZImplications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway0886-022X1525-604910.1080/0886022X.2021.1994994https://doaj.org/article/0eb8b3087b41420098b4534191fa9c332021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/0886022X.2021.1994994https://doaj.org/toc/0886-022Xhttps://doaj.org/toc/1525-6049Objective The aim of present report was to elucidate the effect of cell division cycle associated 4 (CDCA4) on the proliferation and apoptosis of Wilm’s tumor cells, and to further evaluate its underlying mechanism. Methods The expression profiles of CDCA4 and clinical information of Wilm’s tumor patients were obtained from public Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database portal. Real-time qPCR and western blot analyses were utilized to determine the expression levels of CDCA4. Gain- and loss-of-function of CDCA4 assays were conducted with transfection technology to investigate the biological role of CDCA4 in Wilm’s tumor cells. Cell counting kit 8 and flow cytometer assays were employed to examine the effect of CDCA4 on the cells proliferation and apoptosis. Protein expression levels of indicated markers in each group of Wilm’s tumor cells were measured by western blot. Results The transcriptional expression of CDCA4 was drastically upregulated in Wilm’s tumor tissues according to the public TARGET database and in Wilm’s tumor cells. The cells viability was remarkably reduced whereas the cells apoptosis was increased in CDCA4-knockdown group compared with negative control group. However, CDCA4-overexpression group promoted the cells proliferation and suppressed the cells apoptosis. Furthermore, the protein expression levels of p-AKT, p-mTOR, and Cyclin D1 were significantly reduced after depletion of CDCA4, whereas overexpression of CDCA4 dramatically elevated these markers’ expression levels. Conclusions CDCA4 is highly expressed in Wilm’s tumor and promoted the proliferation whereas inhibited the apoptosis of Wilm’s tumor cells through activating the AKT/mTOR signaling pathway.Suqing LiCong QinYike ChenDan WeiZhijun TanJiadong MengTaylor & Francis Grouparticlecdca4nephroblastomaproliferationapoptosisDiseases of the genitourinary system. UrologyRC870-923ENRenal Failure, Vol 43, Iss 1, Pp 1470-1478 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cdca4 nephroblastoma proliferation apoptosis Diseases of the genitourinary system. Urology RC870-923
spellingShingle	cdca4 nephroblastoma proliferation apoptosis Diseases of the genitourinary system. Urology RC870-923 Suqing Li Cong Qin Yike Chen Dan Wei Zhijun Tan Jiadong Meng Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway
description	Objective The aim of present report was to elucidate the effect of cell division cycle associated 4 (CDCA4) on the proliferation and apoptosis of Wilm’s tumor cells, and to further evaluate its underlying mechanism. Methods The expression profiles of CDCA4 and clinical information of Wilm’s tumor patients were obtained from public Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database portal. Real-time qPCR and western blot analyses were utilized to determine the expression levels of CDCA4. Gain- and loss-of-function of CDCA4 assays were conducted with transfection technology to investigate the biological role of CDCA4 in Wilm’s tumor cells. Cell counting kit 8 and flow cytometer assays were employed to examine the effect of CDCA4 on the cells proliferation and apoptosis. Protein expression levels of indicated markers in each group of Wilm’s tumor cells were measured by western blot. Results The transcriptional expression of CDCA4 was drastically upregulated in Wilm’s tumor tissues according to the public TARGET database and in Wilm’s tumor cells. The cells viability was remarkably reduced whereas the cells apoptosis was increased in CDCA4-knockdown group compared with negative control group. However, CDCA4-overexpression group promoted the cells proliferation and suppressed the cells apoptosis. Furthermore, the protein expression levels of p-AKT, p-mTOR, and Cyclin D1 were significantly reduced after depletion of CDCA4, whereas overexpression of CDCA4 dramatically elevated these markers’ expression levels. Conclusions CDCA4 is highly expressed in Wilm’s tumor and promoted the proliferation whereas inhibited the apoptosis of Wilm’s tumor cells through activating the AKT/mTOR signaling pathway.
format	article
author	Suqing Li Cong Qin Yike Chen Dan Wei Zhijun Tan Jiadong Meng
author_facet	Suqing Li Cong Qin Yike Chen Dan Wei Zhijun Tan Jiadong Meng
author_sort	Suqing Li
title	Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway
title_short	Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway
title_full	Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway
title_fullStr	Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway
title_full_unstemmed	Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway
title_sort	implications of cell division cycle associated 4 on the wilm’s tumor cells viability via akt/mtor signaling pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/0eb8b3087b41420098b4534191fa9c33
work_keys_str_mv	AT suqingli implicationsofcelldivisioncycleassociated4onthewilmstumorcellsviabilityviaaktmtorsignalingpathway AT congqin implicationsofcelldivisioncycleassociated4onthewilmstumorcellsviabilityviaaktmtorsignalingpathway AT yikechen implicationsofcelldivisioncycleassociated4onthewilmstumorcellsviabilityviaaktmtorsignalingpathway AT danwei implicationsofcelldivisioncycleassociated4onthewilmstumorcellsviabilityviaaktmtorsignalingpathway AT zhijuntan implicationsofcelldivisioncycleassociated4onthewilmstumorcellsviabilityviaaktmtorsignalingpathway AT jiadongmeng implicationsofcelldivisioncycleassociated4onthewilmstumorcellsviabilityviaaktmtorsignalingpathway
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Implications of cell division cycle associated 4 on the Wilm’s tumor cells viability via AKT/mTOR signaling pathway

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