Long-term stability of cortical ensembles
Neuronal ensembles, coactive groups of neurons found in spontaneous and evoked cortical activity, are causally related to memories and perception, but it is still unknown how stable or flexible they are over time. We used two-photon multiplane calcium imaging to track over weeks the activity of the...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:0eb9184eca7b4484a5ef94bc3a9d3d4e2021-11-25T14:34:50ZLong-term stability of cortical ensembles10.7554/eLife.644492050-084Xe64449https://doaj.org/article/0eb9184eca7b4484a5ef94bc3a9d3d4e2021-07-01T00:00:00Zhttps://elifesciences.org/articles/64449https://doaj.org/toc/2050-084XNeuronal ensembles, coactive groups of neurons found in spontaneous and evoked cortical activity, are causally related to memories and perception, but it is still unknown how stable or flexible they are over time. We used two-photon multiplane calcium imaging to track over weeks the activity of the same pyramidal neurons in layer 2/3 of the visual cortex from awake mice and recorded their spontaneous and visually evoked responses. Less than half of the neurons remained active across any two imaging sessions. These stable neurons formed ensembles that lasted weeks, but some ensembles were also transient and appeared only in one single session. Stable ensembles preserved most of their neurons for up to 46 days, our longest imaged period, and these ‘core’ cells had stronger functional connectivity. Our results demonstrate that neuronal ensembles can last for weeks and could, in principle, serve as a substrate for long-lasting representation of perceptual states or memories.Jesús Pérez-OrtegaTzitzitlini Alejandre-GarcíaRafael YusteeLife Sciences Publications Ltdarticleensemblesvisual cortexstabilitymemorytwo-photonmultiplaneMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
ensembles visual cortex stability memory two-photon multiplane Medicine R Science Q Biology (General) QH301-705.5 |
| spellingShingle |
ensembles visual cortex stability memory two-photon multiplane Medicine R Science Q Biology (General) QH301-705.5 Jesús Pérez-Ortega Tzitzitlini Alejandre-García Rafael Yuste Long-term stability of cortical ensembles |
| description |
Neuronal ensembles, coactive groups of neurons found in spontaneous and evoked cortical activity, are causally related to memories and perception, but it is still unknown how stable or flexible they are over time. We used two-photon multiplane calcium imaging to track over weeks the activity of the same pyramidal neurons in layer 2/3 of the visual cortex from awake mice and recorded their spontaneous and visually evoked responses. Less than half of the neurons remained active across any two imaging sessions. These stable neurons formed ensembles that lasted weeks, but some ensembles were also transient and appeared only in one single session. Stable ensembles preserved most of their neurons for up to 46 days, our longest imaged period, and these ‘core’ cells had stronger functional connectivity. Our results demonstrate that neuronal ensembles can last for weeks and could, in principle, serve as a substrate for long-lasting representation of perceptual states or memories. |
| format |
article |
| author |
Jesús Pérez-Ortega Tzitzitlini Alejandre-García Rafael Yuste |
| author_facet |
Jesús Pérez-Ortega Tzitzitlini Alejandre-García Rafael Yuste |
| author_sort |
Jesús Pérez-Ortega |
| title |
Long-term stability of cortical ensembles |
| title_short |
Long-term stability of cortical ensembles |
| title_full |
Long-term stability of cortical ensembles |
| title_fullStr |
Long-term stability of cortical ensembles |
| title_full_unstemmed |
Long-term stability of cortical ensembles |
| title_sort |
long-term stability of cortical ensembles |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/0eb9184eca7b4484a5ef94bc3a9d3d4e |
| work_keys_str_mv |
AT jesusperezortega longtermstabilityofcorticalensembles AT tzitzitlinialejandregarcia longtermstabilityofcorticalensembles AT rafaelyuste longtermstabilityofcorticalensembles |
| _version_ |
1718413361875517440 |