Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas.
T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that γδ T cells mediate antigen-specific killing of tumor cells, we studied the representation and the in vitro cytokine production and cytotoxic activity of tumor infiltrating γδ T cells from 74 patients wit...
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2012
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oai:doaj.org-article:0eb98e24e0154711873f3b3ca5ed46bf2021-11-18T08:07:36ZCharacterization of human γδ T lymphocytes infiltrating primary malignant melanomas.1932-620310.1371/journal.pone.0049878https://doaj.org/article/0eb98e24e0154711873f3b3ca5ed46bf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23189169/?tool=EBIhttps://doaj.org/toc/1932-6203T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that γδ T cells mediate antigen-specific killing of tumor cells, we studied the representation and the in vitro cytokine production and cytotoxic activity of tumor infiltrating γδ T cells from 74 patients with primary melanoma. We found that γδ T cells represent the major lymphocyte population infiltrating melanoma, and both Vδ1(+) and Vδ2(+) cells are involved. The majority of melanoma-infiltrating γδ cells showed effector memory and terminally-differentiated phenotypes and, accordingly, polyclonal γδ T cell lines obtained from tumor-infiltrating immune cells produced IFN-γ and TNF-α and were capable of killing melanoma cell lines in vitro. The cytotoxic capability of Vδ2 cell lines was further improved by pre-treatment of tumor target cells with zoledronate. Moreover, higher rate of γδ T cells isolation and percentages of Vδ2 cells correlate with early stage of development of melanoma and absence of metastasis. Altogether, our results suggest that a natural immune response mediated by γδ T lymphocytes may contribute to the immunosurveillance of melanoma.Adriana CordovaFrancesca ToiaCarmela La MendolaValentina OrlandoSerena MeravigliaGaetana RinaldiMatilde TodaroGiuseppe CiceroLeonardo ZichichiPaolo Li DonniNadia CaccamoGiorgio StassiFrancesco DieliFrancesco MoschellaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49878 (2012) |
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Medicine R Science Q |
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Medicine R Science Q Adriana Cordova Francesca Toia Carmela La Mendola Valentina Orlando Serena Meraviglia Gaetana Rinaldi Matilde Todaro Giuseppe Cicero Leonardo Zichichi Paolo Li Donni Nadia Caccamo Giorgio Stassi Francesco Dieli Francesco Moschella Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. |
| description |
T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that γδ T cells mediate antigen-specific killing of tumor cells, we studied the representation and the in vitro cytokine production and cytotoxic activity of tumor infiltrating γδ T cells from 74 patients with primary melanoma. We found that γδ T cells represent the major lymphocyte population infiltrating melanoma, and both Vδ1(+) and Vδ2(+) cells are involved. The majority of melanoma-infiltrating γδ cells showed effector memory and terminally-differentiated phenotypes and, accordingly, polyclonal γδ T cell lines obtained from tumor-infiltrating immune cells produced IFN-γ and TNF-α and were capable of killing melanoma cell lines in vitro. The cytotoxic capability of Vδ2 cell lines was further improved by pre-treatment of tumor target cells with zoledronate. Moreover, higher rate of γδ T cells isolation and percentages of Vδ2 cells correlate with early stage of development of melanoma and absence of metastasis. Altogether, our results suggest that a natural immune response mediated by γδ T lymphocytes may contribute to the immunosurveillance of melanoma. |
| format |
article |
| author |
Adriana Cordova Francesca Toia Carmela La Mendola Valentina Orlando Serena Meraviglia Gaetana Rinaldi Matilde Todaro Giuseppe Cicero Leonardo Zichichi Paolo Li Donni Nadia Caccamo Giorgio Stassi Francesco Dieli Francesco Moschella |
| author_facet |
Adriana Cordova Francesca Toia Carmela La Mendola Valentina Orlando Serena Meraviglia Gaetana Rinaldi Matilde Todaro Giuseppe Cicero Leonardo Zichichi Paolo Li Donni Nadia Caccamo Giorgio Stassi Francesco Dieli Francesco Moschella |
| author_sort |
Adriana Cordova |
| title |
Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. |
| title_short |
Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. |
| title_full |
Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. |
| title_fullStr |
Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. |
| title_full_unstemmed |
Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. |
| title_sort |
characterization of human γδ t lymphocytes infiltrating primary malignant melanomas. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/0eb98e24e0154711873f3b3ca5ed46bf |
| work_keys_str_mv |
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| _version_ |
1718422151886798848 |