Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients

Introduction: Gastric cancer (GC) is the third leading cause of cancer death and a major public health-care problem worldwide. At present, methods for plasma detection of cancer are limited. MicroRNAs (miRNAs) have recently been proposed as genetic regulators, which are deregulated in different type...

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Autores principales:	Hugo Cuellar-Gomez, Ma Esther Ocharán-Hernández, Claudia C. Calzada-Mendoza, David A. Comoto-Santacruz
Formato:	article
Lenguaje:	EN ES
Publicado:	Permanyer 2021
Materias:	Gastric cancer. MicroRNAs. Plasma. Biomarker. Diagnosis. Surgery RD1-811
Acceso en línea:	https://doaj.org/article/0eb9efce98b845ceac8561c35a033f9f
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spelling	oai:doaj.org-article:0eb9efce98b845ceac8561c35a033f9f2021-12-01T20:30:18ZSerum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients10.24875/CIRU.200009632444-054Xhttps://doaj.org/article/0eb9efce98b845ceac8561c35a033f9f2021-01-01T00:00:00Zhttps://www.cirugiaycirujanos.com/frame_esp.php?id=577https://doaj.org/toc/2444-054XIntroduction: Gastric cancer (GC) is the third leading cause of cancer death and a major public health-care problem worldwide. At present, methods for plasma detection of cancer are limited. MicroRNAs (miRNAs) have recently been proposed as genetic regulators, which are deregulated in different types of cancer. The miRNAs are stable in serum/plasma and can be detected. Circulating miRNAs in plasma have been proposed as potential diagnostic biomarkers in GC. Materials and methods: After reviewing the relevant literature, the expression levels of seven miRNAs (miR-16, miR-21, miR-25, miR-26a, miR-92, miR-218, miR-223, and miR-451) were assessed by quantitative reverse transcription polymerase chain reaction using TaqMan microRNA Assays (Applied Biosystems) in plasma samples from GC patients (n = 80) and healthy controls (n = 80). Results: Our results demonstrated that the expression levels of miR-21 and miR-25 were significantly upregulated in GC patients compared to healthy controls with a Fold Change of 11.551 and 60.129, respectively, while miR-223 showed downregulation in GC patients compared to healthy controls with a Fold Change of −247.281. The absolute value of Fold Change > 2 was consider significant, p < 0.05. Conclusions: Our results indicated that miR-21, miR-25, and miR-223 in plasma samples can be served as a potential noninvasive tool in detection of GC. Hugo Cuellar-GomezMa Esther Ocharán-HernándezClaudia C. Calzada-MendozaDavid A. Comoto-SantacruzPermanyerarticleGastric cancer. MicroRNAs. Plasma. Biomarker. Diagnosis.SurgeryRD1-811ENESCirugía y Cirujanos, Vol 89, Iss 6 (2021)
institution	DOAJ
collection	DOAJ
language	EN ES
topic	Gastric cancer. MicroRNAs. Plasma. Biomarker. Diagnosis. Surgery RD1-811
spellingShingle	Gastric cancer. MicroRNAs. Plasma. Biomarker. Diagnosis. Surgery RD1-811 Hugo Cuellar-Gomez Ma Esther Ocharán-Hernández Claudia C. Calzada-Mendoza David A. Comoto-Santacruz Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients
description	Introduction: Gastric cancer (GC) is the third leading cause of cancer death and a major public health-care problem worldwide. At present, methods for plasma detection of cancer are limited. MicroRNAs (miRNAs) have recently been proposed as genetic regulators, which are deregulated in different types of cancer. The miRNAs are stable in serum/plasma and can be detected. Circulating miRNAs in plasma have been proposed as potential diagnostic biomarkers in GC. Materials and methods: After reviewing the relevant literature, the expression levels of seven miRNAs (miR-16, miR-21, miR-25, miR-26a, miR-92, miR-218, miR-223, and miR-451) were assessed by quantitative reverse transcription polymerase chain reaction using TaqMan microRNA Assays (Applied Biosystems) in plasma samples from GC patients (n = 80) and healthy controls (n = 80). Results: Our results demonstrated that the expression levels of miR-21 and miR-25 were significantly upregulated in GC patients compared to healthy controls with a Fold Change of 11.551 and 60.129, respectively, while miR-223 showed downregulation in GC patients compared to healthy controls with a Fold Change of −247.281. The absolute value of Fold Change > 2 was consider significant, p < 0.05. Conclusions: Our results indicated that miR-21, miR-25, and miR-223 in plasma samples can be served as a potential noninvasive tool in detection of GC.
format	article
author	Hugo Cuellar-Gomez Ma Esther Ocharán-Hernández Claudia C. Calzada-Mendoza David A. Comoto-Santacruz
author_facet	Hugo Cuellar-Gomez Ma Esther Ocharán-Hernández Claudia C. Calzada-Mendoza David A. Comoto-Santacruz
author_sort	Hugo Cuellar-Gomez
title	Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients
title_short	Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients
title_full	Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients
title_fullStr	Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients
title_full_unstemmed	Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients
title_sort	serum mirna profile as a potential tool for non-invasive gastric cancer diagnosis in mexican patients
publisher	Permanyer
publishDate	2021
url	https://doaj.org/article/0eb9efce98b845ceac8561c35a033f9f
work_keys_str_mv	AT hugocuellargomez serummirnaprofileasapotentialtoolfornoninvasivegastriccancerdiagnosisinmexicanpatients AT maestherocharanhernandez serummirnaprofileasapotentialtoolfornoninvasivegastriccancerdiagnosisinmexicanpatients AT claudiaccalzadamendoza serummirnaprofileasapotentialtoolfornoninvasivegastriccancerdiagnosisinmexicanpatients AT davidacomotosantacruz serummirnaprofileasapotentialtoolfornoninvasivegastriccancerdiagnosisinmexicanpatients
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Serum miRNA profile as a potential tool for non-invasive gastric cancer diagnosis in Mexican patients

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