Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.

Various semen extender formulas are in use to maintain sperm longevity and quality whilst acting against bacterial contamination in liquid sperm preservation. Aminoglycosides are commonly supplemented to aid in the control of bacteria. As bacterial resistance is increasing worldwide, antimicrobial p...

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Autores principales:	Stephanie Speck, Alexandre Courtiol, Christof Junkes, Margitta Dathe, Karin Müller, Martin Schulze
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ebb0ee5df434e279679eaa145f3f6ab
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spelling	oai:doaj.org-article:0ebb0ee5df434e279679eaa145f3f6ab2021-11-25T06:03:33ZCationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.1932-620310.1371/journal.pone.0105949https://doaj.org/article/0ebb0ee5df434e279679eaa145f3f6ab2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25148109/?tool=EBIhttps://doaj.org/toc/1932-6203Various semen extender formulas are in use to maintain sperm longevity and quality whilst acting against bacterial contamination in liquid sperm preservation. Aminoglycosides are commonly supplemented to aid in the control of bacteria. As bacterial resistance is increasing worldwide, antimicrobial peptides (AMPs) received lively interest as alternatives to overcome multi-drug resistant bacteria. We investigated, whether synthetic cationic AMPs might be a suitable alternative for conventional antibiotics in liquid boar sperm preservation. The antibacterial activity of two cyclic AMPs (c-WWW, c-WFW) and a helical magainin II amide analog (MK5E) was studied in vitro against two Gram-positive and eleven Gram-negative bacteria. Isolates included ATCC reference strains, multi-resistant E. coli and bacteria cultured from boar semen. Using broth microdilution, minimum inhibitory concentrations were determined for all AMPs. All AMPs revealed activity towards the majority of bacteria but not against Proteus spp. (all AMPs) and Staphylococcus aureus ATCC 29213 (MK5E). We could also demonstrate that c-WWW and c-WFW were effective against bacterial growth in liquid preserved boar semen in situ, especially when combined with a small amount of gentamicin. Our results suggest that albeit not offering a complete alternative to traditional antibiotics, the use of AMPs offers a promising solution to decrease the use of conventional antibiotics and thereby limit the selection of multi-resistant strains.Stephanie SpeckAlexandre CourtiolChristof JunkesMargitta DatheKarin MüllerMartin SchulzePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105949 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephanie Speck Alexandre Courtiol Christof Junkes Margitta Dathe Karin Müller Martin Schulze Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
description	Various semen extender formulas are in use to maintain sperm longevity and quality whilst acting against bacterial contamination in liquid sperm preservation. Aminoglycosides are commonly supplemented to aid in the control of bacteria. As bacterial resistance is increasing worldwide, antimicrobial peptides (AMPs) received lively interest as alternatives to overcome multi-drug resistant bacteria. We investigated, whether synthetic cationic AMPs might be a suitable alternative for conventional antibiotics in liquid boar sperm preservation. The antibacterial activity of two cyclic AMPs (c-WWW, c-WFW) and a helical magainin II amide analog (MK5E) was studied in vitro against two Gram-positive and eleven Gram-negative bacteria. Isolates included ATCC reference strains, multi-resistant E. coli and bacteria cultured from boar semen. Using broth microdilution, minimum inhibitory concentrations were determined for all AMPs. All AMPs revealed activity towards the majority of bacteria but not against Proteus spp. (all AMPs) and Staphylococcus aureus ATCC 29213 (MK5E). We could also demonstrate that c-WWW and c-WFW were effective against bacterial growth in liquid preserved boar semen in situ, especially when combined with a small amount of gentamicin. Our results suggest that albeit not offering a complete alternative to traditional antibiotics, the use of AMPs offers a promising solution to decrease the use of conventional antibiotics and thereby limit the selection of multi-resistant strains.
format	article
author	Stephanie Speck Alexandre Courtiol Christof Junkes Margitta Dathe Karin Müller Martin Schulze
author_facet	Stephanie Speck Alexandre Courtiol Christof Junkes Margitta Dathe Karin Müller Martin Schulze
author_sort	Stephanie Speck
title	Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
title_short	Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
title_full	Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
title_fullStr	Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
title_full_unstemmed	Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
title_sort	cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0ebb0ee5df434e279679eaa145f3f6ab
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Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.

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