Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study

Abstract Background Osteonecrosis of the femoral head (ONFH) is a common but intractable disease that appears to involve lipid metabolic disorders. Although numerous studies have demonstrated that high blood levels of low-density lipoprotein (LDL) are closely associated with ONFH, there is limited e...

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Autores principales:	Xin-Yuan Wang, Tian-Le Ma, Kang-Ning Chen, Zhi-Ying Pang, Hao Wang, Jun-Ming Huang, Guo-Bin Qi, Chen-Zhong Wang, Zeng-Xin Jiang, Lin-Jing Gong, Zhe Wang, Chang Jiang, Zuo-Qin Yan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Osteonecrosis of the femoral head Low-density lipoprotein Oxidized low-density lipoprotein Osteocyte Hypoxia Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/0ec2d62544e84a08b88268df5151479d
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spelling	oai:doaj.org-article:0ec2d62544e84a08b88268df5151479d2021-11-28T12:39:00ZAccumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study10.1186/s12944-021-01601-x1476-511Xhttps://doaj.org/article/0ec2d62544e84a08b88268df5151479d2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12944-021-01601-xhttps://doaj.org/toc/1476-511XAbstract Background Osteonecrosis of the femoral head (ONFH) is a common but intractable disease that appears to involve lipid metabolic disorders. Although numerous studies have demonstrated that high blood levels of low-density lipoprotein (LDL) are closely associated with ONFH, there is limited evidence to explain the pathological role of LDL. Pathological and in vitro studies were performed to investigate the role of disordered metabolism of LDL and oxidized LDL (ox-LDL) in the femoral head in the pathology of ONFH. Methods Nineteen femoral head specimens from patients with ONFH were obtained for immunohistochemistry analysis. Murine long-bone osteocyte Y4 cells were used to study the effects of LDL/ox-LDL on cell viability, apoptosis, and metabolism process of LDL/ox-LDL in osteocytes in normoxic and hypoxic environments. Results In the pathological specimens, marked accumulation of LDL/ox-LDL was observed in osteocytes/lacunae of necrotic regions compared with healthy regions. In vitro studies showed that ox-LDL, rather than LDL, reduced the viability and enhanced apoptosis of osteocytes. Pathological sections indicated that the accumulation of ox-LDL was significantly associated with impaired blood supply. Exposure to a hypoxic environment appeared to be a key factor leading to LDL/ox-LDL accumulation by enhancing internalisation and oxidation of LDL in osteocytes. Conclusions The accumulation of LDL/ox-LDL in the necrotic region may contribute to the pathology of ONFH. These findings could provide new insights into the prevention and treatment of ONFH.Xin-Yuan WangTian-Le MaKang-Ning ChenZhi-Ying PangHao WangJun-Ming HuangGuo-Bin QiChen-Zhong WangZeng-Xin JiangLin-Jing GongZhe WangChang JiangZuo-Qin YanBMCarticleOsteonecrosis of the femoral headLow-density lipoproteinOxidized low-density lipoproteinOsteocyteHypoxiaNutritional diseases. Deficiency diseasesRC620-627ENLipids in Health and Disease, Vol 20, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteonecrosis of the femoral head Low-density lipoprotein Oxidized low-density lipoprotein Osteocyte Hypoxia Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	Osteonecrosis of the femoral head Low-density lipoprotein Oxidized low-density lipoprotein Osteocyte Hypoxia Nutritional diseases. Deficiency diseases RC620-627 Xin-Yuan Wang Tian-Le Ma Kang-Ning Chen Zhi-Ying Pang Hao Wang Jun-Ming Huang Guo-Bin Qi Chen-Zhong Wang Zeng-Xin Jiang Lin-Jing Gong Zhe Wang Chang Jiang Zuo-Qin Yan Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
description	Abstract Background Osteonecrosis of the femoral head (ONFH) is a common but intractable disease that appears to involve lipid metabolic disorders. Although numerous studies have demonstrated that high blood levels of low-density lipoprotein (LDL) are closely associated with ONFH, there is limited evidence to explain the pathological role of LDL. Pathological and in vitro studies were performed to investigate the role of disordered metabolism of LDL and oxidized LDL (ox-LDL) in the femoral head in the pathology of ONFH. Methods Nineteen femoral head specimens from patients with ONFH were obtained for immunohistochemistry analysis. Murine long-bone osteocyte Y4 cells were used to study the effects of LDL/ox-LDL on cell viability, apoptosis, and metabolism process of LDL/ox-LDL in osteocytes in normoxic and hypoxic environments. Results In the pathological specimens, marked accumulation of LDL/ox-LDL was observed in osteocytes/lacunae of necrotic regions compared with healthy regions. In vitro studies showed that ox-LDL, rather than LDL, reduced the viability and enhanced apoptosis of osteocytes. Pathological sections indicated that the accumulation of ox-LDL was significantly associated with impaired blood supply. Exposure to a hypoxic environment appeared to be a key factor leading to LDL/ox-LDL accumulation by enhancing internalisation and oxidation of LDL in osteocytes. Conclusions The accumulation of LDL/ox-LDL in the necrotic region may contribute to the pathology of ONFH. These findings could provide new insights into the prevention and treatment of ONFH.
format	article
author	Xin-Yuan Wang Tian-Le Ma Kang-Ning Chen Zhi-Ying Pang Hao Wang Jun-Ming Huang Guo-Bin Qi Chen-Zhong Wang Zeng-Xin Jiang Lin-Jing Gong Zhe Wang Chang Jiang Zuo-Qin Yan
author_facet	Xin-Yuan Wang Tian-Le Ma Kang-Ning Chen Zhi-Ying Pang Hao Wang Jun-Ming Huang Guo-Bin Qi Chen-Zhong Wang Zeng-Xin Jiang Lin-Jing Gong Zhe Wang Chang Jiang Zuo-Qin Yan
author_sort	Xin-Yuan Wang
title	Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
title_short	Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
title_full	Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
title_fullStr	Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
title_full_unstemmed	Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
title_sort	accumulation of ldl/ox-ldl in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0ec2d62544e84a08b88268df5151479d
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Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study

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