The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions

Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice...

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Autores principales: Turhadi, Miftahuddin, Hamim, Munif Ghulamahdi
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Publicado: Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah 2021
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spelling oai:doaj.org-article:0eca70ae86ab4dc7a48e4fe3a33a801f2021-11-22T13:46:40ZThe Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions2614-154X2614-155810.22236/j.bes/516395https://doaj.org/article/0eca70ae86ab4dc7a48e4fe3a33a801f2021-04-01T00:00:00Zhttps://journal.uhamka.ac.id/index.php/bioeduscience/article/view/6395https://doaj.org/toc/2614-154Xhttps://doaj.org/toc/2614-1558Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida’s Half-Strength solution (HSY), Yoshida’s Half-Strength + 0.2% agar solution (HSYA), and Yoshida’s Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.TurhadiMiftahuddinHamimMunif GhulamahdiDepartment of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyaharticleagar solutionleaf bronzingriceEducationLBiology (General)QH301-705.5ENIDBioeduscience, Vol 5, Iss 1, Pp 24-29 (2021)
institution DOAJ
collection DOAJ
language EN
ID
topic agar solution
leaf bronzing
rice
Education
L
Biology (General)
QH301-705.5
spellingShingle agar solution
leaf bronzing
rice
Education
L
Biology (General)
QH301-705.5
Turhadi
Miftahuddin
Hamim
Munif Ghulamahdi
The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
description Background: Evaluation of the tolerance level of rice to iron (Fe) toxicity stress can be done using a hydroponic system in a nutrient culture solution under a controlled condition. This study aimed to obtain a nutrient culture solution that effective as a medium for evaluating the response of rice under Fe toxicity stress condition. Methods: This experiment was carried out by comparing the effectiveness of three kinds of nutrient culture media, namely Yoshida’s Half-Strength solution (HSY), Yoshida’s Half-Strength + 0.2% agar solution (HSYA), and Yoshida’s Full-Strength + 0.2% agar solution (FSYA) using two rice genotypes, Inpara 5 (sensitive to Fe toxicity) and Mahsuri (tolerant to Fe toxicity). Leaf bronzing level, plant dry weight, and pH of nutrient culture media were observed in this experiment. Results: The results showed that the stress response as represented by the bronzing score in Inpara 5 leaves was known to be higher than that of Mahsuri in the three nutrient culture media. The decrease of root and shoot dry weight in Inpara 5 was higher than that of Mahsuri. In addition, the decrease in the pH of nutrient culture solution media without an agar addition (HSY) occurred faster than the media with the agar addition (HSYA and FSYA). Conclusion: The HSYA and FSYA media exhibited a similar pattern of pH declining but causing significant differences in growth responses between Inpara 5 and Mashuri indicating the HSYA medium is considered more efficient compared to the FSYA medium because it only requires a smaller amount of agar.
format article
author Turhadi
Miftahuddin
Hamim
Munif Ghulamahdi
author_facet Turhadi
Miftahuddin
Hamim
Munif Ghulamahdi
author_sort Turhadi
title The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
title_short The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
title_full The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
title_fullStr The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
title_full_unstemmed The Effectiveness of Nutrient Culture Solutions with Agar Addition as An Evaluation Media of Rice Under Iron Toxicity Conditions
title_sort effectiveness of nutrient culture solutions with agar addition as an evaluation media of rice under iron toxicity conditions
publisher Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah
publishDate 2021
url https://doaj.org/article/0eca70ae86ab4dc7a48e4fe3a33a801f
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