Mapping the small RNA content of simian immunodeficiency virions (SIV).

Recent evidence indicates that regulatory small non-coding RNAs are not only components of eukaryotic cells and vesicles, but also reside within a number of different viruses including retroviral particles. Using ultra-deep sequencing we have comprehensively analyzed the content of simian immunodefi...

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Autores principales:	Markus Brameier, Wiebke Ibing, Katharina Höfer, Judith Montag, Christiane Stahl-Hennig, Dirk Motzkus
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ed141c9b4b54837bdaa001f113bc20c
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spelling	oai:doaj.org-article:0ed141c9b4b54837bdaa001f113bc20c2021-11-18T08:54:08ZMapping the small RNA content of simian immunodeficiency virions (SIV).1932-620310.1371/journal.pone.0075063https://doaj.org/article/0ed141c9b4b54837bdaa001f113bc20c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086438/?tool=EBIhttps://doaj.org/toc/1932-6203Recent evidence indicates that regulatory small non-coding RNAs are not only components of eukaryotic cells and vesicles, but also reside within a number of different viruses including retroviral particles. Using ultra-deep sequencing we have comprehensively analyzed the content of simian immunodeficiency virions (SIV), which were compared to mock-control preparations. Our analysis revealed that more than 428,000 sequence reads matched the SIV mac239 genome sequence. Among these we could identify 12 virus-derived small RNAs (vsRNAs) that were highly abundant. Beside known retrovirus-enriched small RNAs, like 7SL-RNA, tRNA(Lys3) and tRNA(Lys) isoacceptors, we also identified defined fragments derived from small ILF3/NF90-associated RNA snaR-A14, that were enriched more than 50 fold in SIV. We also found evidence that small nucleolar RNAs U2 and U12 were underrepresented in the SIV preparation, indicating that the relative number or the content of co-isolated exosomes was changed upon infection. Our comprehensive atlas of SIV-incorporated small RNAs provides a refined picture of the composition of retrovirions, which gives novel insights into viral packaging.Markus BrameierWiebke IbingKatharina HöferJudith MontagChristiane Stahl-HennigDirk MotzkusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75063 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Markus Brameier Wiebke Ibing Katharina Höfer Judith Montag Christiane Stahl-Hennig Dirk Motzkus Mapping the small RNA content of simian immunodeficiency virions (SIV).
description	Recent evidence indicates that regulatory small non-coding RNAs are not only components of eukaryotic cells and vesicles, but also reside within a number of different viruses including retroviral particles. Using ultra-deep sequencing we have comprehensively analyzed the content of simian immunodeficiency virions (SIV), which were compared to mock-control preparations. Our analysis revealed that more than 428,000 sequence reads matched the SIV mac239 genome sequence. Among these we could identify 12 virus-derived small RNAs (vsRNAs) that were highly abundant. Beside known retrovirus-enriched small RNAs, like 7SL-RNA, tRNA(Lys3) and tRNA(Lys) isoacceptors, we also identified defined fragments derived from small ILF3/NF90-associated RNA snaR-A14, that were enriched more than 50 fold in SIV. We also found evidence that small nucleolar RNAs U2 and U12 were underrepresented in the SIV preparation, indicating that the relative number or the content of co-isolated exosomes was changed upon infection. Our comprehensive atlas of SIV-incorporated small RNAs provides a refined picture of the composition of retrovirions, which gives novel insights into viral packaging.
format	article
author	Markus Brameier Wiebke Ibing Katharina Höfer Judith Montag Christiane Stahl-Hennig Dirk Motzkus
author_facet	Markus Brameier Wiebke Ibing Katharina Höfer Judith Montag Christiane Stahl-Hennig Dirk Motzkus
author_sort	Markus Brameier
title	Mapping the small RNA content of simian immunodeficiency virions (SIV).
title_short	Mapping the small RNA content of simian immunodeficiency virions (SIV).
title_full	Mapping the small RNA content of simian immunodeficiency virions (SIV).
title_fullStr	Mapping the small RNA content of simian immunodeficiency virions (SIV).
title_full_unstemmed	Mapping the small RNA content of simian immunodeficiency virions (SIV).
title_sort	mapping the small rna content of simian immunodeficiency virions (siv).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0ed141c9b4b54837bdaa001f113bc20c
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Mapping the small RNA content of simian immunodeficiency virions (SIV).

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