Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.

The facultative anaerobe, Bacillus cereus, causes diarrheal diseases in humans. Its ability to deal with oxygen availability is recognized to be critical for pathogenesis. The B. cereus genome comprises a gene encoding a protein with high similarities to the redox regulator, Rex, which is a central...

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Autores principales:	Sabrina Laouami, Géremy Clair, Jean Armengaud, Catherine Duport
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ed46592bcb244b889b17942e20b2922
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spelling	oai:doaj.org-article:0ed46592bcb244b889b17942e20b29222021-11-25T06:00:53ZProteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.1932-620310.1371/journal.pone.0107354https://doaj.org/article/0ed46592bcb244b889b17942e20b29222014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107354https://doaj.org/toc/1932-6203The facultative anaerobe, Bacillus cereus, causes diarrheal diseases in humans. Its ability to deal with oxygen availability is recognized to be critical for pathogenesis. The B. cereus genome comprises a gene encoding a protein with high similarities to the redox regulator, Rex, which is a central regulator of anaerobic metabolism in Bacillus subtilis and other Gram-positive bacteria. Here, we showed that B. cereus rex is monocistronic and down-regulated in the absence of oxygen. The protein encoded by rex is an authentic Rex transcriptional factor since its DNA binding activity depends on the NADH/NAD+ ratio. Rex deletion compromised the ability of B. cereus to cope with external oxidative stress under anaerobiosis while increasing B. cereus resistance against such stress under aerobiosis. The deletion of rex affects anaerobic fermentative and aerobic respiratory metabolism of B. cereus by decreasing and increasing, respectively, the carbon flux through the NADH-recycling lactate pathway. We compared both the cellular proteome and exoproteome of the wild-type and Δrex cells using a high throughput shotgun label-free quantitation approach and identified proteins that are under control of Rex-mediated regulation. Proteomics data have been deposited to the ProteomeXchange with identifier PXD000886. The data suggest that Rex regulates both the cross-talk between metabolic pathways that produce NADH and NADPH and toxinogenesis, especially in oxic conditions.Sabrina LaouamiGéremy ClairJean ArmengaudCatherine DuportPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107354 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabrina Laouami Géremy Clair Jean Armengaud Catherine Duport Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
description	The facultative anaerobe, Bacillus cereus, causes diarrheal diseases in humans. Its ability to deal with oxygen availability is recognized to be critical for pathogenesis. The B. cereus genome comprises a gene encoding a protein with high similarities to the redox regulator, Rex, which is a central regulator of anaerobic metabolism in Bacillus subtilis and other Gram-positive bacteria. Here, we showed that B. cereus rex is monocistronic and down-regulated in the absence of oxygen. The protein encoded by rex is an authentic Rex transcriptional factor since its DNA binding activity depends on the NADH/NAD+ ratio. Rex deletion compromised the ability of B. cereus to cope with external oxidative stress under anaerobiosis while increasing B. cereus resistance against such stress under aerobiosis. The deletion of rex affects anaerobic fermentative and aerobic respiratory metabolism of B. cereus by decreasing and increasing, respectively, the carbon flux through the NADH-recycling lactate pathway. We compared both the cellular proteome and exoproteome of the wild-type and Δrex cells using a high throughput shotgun label-free quantitation approach and identified proteins that are under control of Rex-mediated regulation. Proteomics data have been deposited to the ProteomeXchange with identifier PXD000886. The data suggest that Rex regulates both the cross-talk between metabolic pathways that produce NADH and NADPH and toxinogenesis, especially in oxic conditions.
format	article
author	Sabrina Laouami Géremy Clair Jean Armengaud Catherine Duport
author_facet	Sabrina Laouami Géremy Clair Jean Armengaud Catherine Duport
author_sort	Sabrina Laouami
title	Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
title_short	Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
title_full	Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
title_fullStr	Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
title_full_unstemmed	Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
title_sort	proteomic evidences for rex regulation of metabolism in toxin-producing bacillus cereus atcc 14579.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0ed46592bcb244b889b17942e20b2922
work_keys_str_mv	AT sabrinalaouami proteomicevidencesforrexregulationofmetabolismintoxinproducingbacilluscereusatcc14579 AT geremyclair proteomicevidencesforrexregulationofmetabolismintoxinproducingbacilluscereusatcc14579 AT jeanarmengaud proteomicevidencesforrexregulationofmetabolismintoxinproducingbacilluscereusatcc14579 AT catherineduport proteomicevidencesforrexregulationofmetabolismintoxinproducingbacilluscereusatcc14579
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Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.

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