Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques

Abstract Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were design...

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Autores principales:	Amir Shamloo, Amin Naghdloo, Mohsen Besanjideh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ed473b20e7f465fa026a5b00d6c8a05
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spelling	oai:doaj.org-article:0ed473b20e7f465fa026a5b00d6c8a052021-12-02T13:51:06ZCancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques10.1038/s41598-021-81661-22045-2322https://doaj.org/article/0ed473b20e7f465fa026a5b00d6c8a052021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81661-2https://doaj.org/toc/2045-2322Abstract Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is reinforced by a stack of two permanent magnets to capture the magnetically labeled target cells at the end of the microchannel. These designs were optimized by numerical simulations and tested experimentally for isolation of MCF-7 human breast cancer cells from the population of mouse fibroblast L929 cells. In order to use the hybrid design, magnetite nanoparticles were attached to the MCF-7 cells through specific Ep-CAM antibodies, and two permanent magnets of 0.34 T were utilized at the downstream of the CEA microchannel. These devices were tested at different disk rotational speeds and it was found that the passive design can isolate MCF-7 cells with a recovery rate of 76% for the rotational speed of 2100 rpm while its hybrid counterpart is able to separate the target cells with a recovery rate of 85% for the rotational speed of 1200 rpm. Although the hybrid design of separator has a better separation efficiency and higher purity, the passive one has no need for a time-consuming process of cell labeling, occupies less space on the disk, and does not impose additional costs and complexity.Amir ShamlooAmin NaghdlooMohsen BesanjidehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amir Shamloo Amin Naghdloo Mohsen Besanjideh Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
description	Abstract Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is reinforced by a stack of two permanent magnets to capture the magnetically labeled target cells at the end of the microchannel. These designs were optimized by numerical simulations and tested experimentally for isolation of MCF-7 human breast cancer cells from the population of mouse fibroblast L929 cells. In order to use the hybrid design, magnetite nanoparticles were attached to the MCF-7 cells through specific Ep-CAM antibodies, and two permanent magnets of 0.34 T were utilized at the downstream of the CEA microchannel. These devices were tested at different disk rotational speeds and it was found that the passive design can isolate MCF-7 cells with a recovery rate of 76% for the rotational speed of 2100 rpm while its hybrid counterpart is able to separate the target cells with a recovery rate of 85% for the rotational speed of 1200 rpm. Although the hybrid design of separator has a better separation efficiency and higher purity, the passive one has no need for a time-consuming process of cell labeling, occupies less space on the disk, and does not impose additional costs and complexity.
format	article
author	Amir Shamloo Amin Naghdloo Mohsen Besanjideh
author_facet	Amir Shamloo Amin Naghdloo Mohsen Besanjideh
author_sort	Amir Shamloo
title	Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_short	Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_full	Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_fullStr	Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_full_unstemmed	Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_sort	cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ed473b20e7f465fa026a5b00d6c8a05
work_keys_str_mv	AT amirshamloo cancercellenrichmentonacentrifugalmicrofluidicplatformusinghydrodynamicandmagnetophoretictechniques AT aminnaghdloo cancercellenrichmentonacentrifugalmicrofluidicplatformusinghydrodynamicandmagnetophoretictechniques AT mohsenbesanjideh cancercellenrichmentonacentrifugalmicrofluidicplatformusinghydrodynamicandmagnetophoretictechniques
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Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques

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