Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay

Lipids are one of the main organic components in industrial and municipal wastewaters. Lipid hydrolysis is the first step in the biological conversion process and requires a close contact between lipid emulsion droplets and microbial hydrolytic enzymes. Adequate lipid hydrolysis monitoring is crucia...

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Autores principales:	P. Van Gaelen, D. Springael, I. Smets
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	biological wastewater treatment enzyme activity hydrolysis lipids rhodamine b Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/0ed688746bd44beb8b551fdba7d552fe
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spelling	oai:doaj.org-article:0ed688746bd44beb8b551fdba7d552fe2021-11-05T21:09:35ZLipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay1751-231X10.2166/wpt.2021.022https://doaj.org/article/0ed688746bd44beb8b551fdba7d552fe2021-04-01T00:00:00Zhttp://wpt.iwaponline.com/content/16/2/605https://doaj.org/toc/1751-231XLipids are one of the main organic components in industrial and municipal wastewaters. Lipid hydrolysis is the first step in the biological conversion process and requires a close contact between lipid emulsion droplets and microbial hydrolytic enzymes. Adequate lipid hydrolysis monitoring is crucial to obtain mechanistic knowledge on lipid hydrolysis in response to changes in the process conditions and to improve the overall lipid conversion efficiency in aerobic and anaerobic wastewater treatment systems. We set out to develop a high-throughput lipid hydrolysis monitoring method based on vegetable oil model substrates and fluorescent quantification of product formation by exploiting the interaction with Rhodamine B. Olive oil and soybean oil emulsions were prepared with a high interfacial area and acceptable emulsion stability. The method was easy to apply and allowed to obtain detailed kinetic data over a time course of several hours for up to 16 samples in parallel. A proof-of-concept was obtained with a commercial enzyme, Amano lipase, but remains to be provided for wastewater treatment sludge samples. The findings of this study pave the way for further method development in lipid hydrolysis monitoring. Highlights Lipid hydrolysis monitoring is essential to optimize (an)aerobic wastewater treatment.; High-throughput monitoring methods are required.; The established p-nitrophenol palmitate method is not adequate.; A lipid assay model substrate should resemble the actual lipids present in wastewater.; Fluorescent lipid hydrolysis quantification with Rhodamine B is a promising approach for high-throughput monitoring.;P. Van GaelenD. SpringaelI. SmetsIWA Publishingarticlebiological wastewater treatmentenzyme activityhydrolysislipidsrhodamine bEnvironmental technology. Sanitary engineeringTD1-1066ENWater Practice and Technology, Vol 16, Iss 2, Pp 605-620 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biological wastewater treatment enzyme activity hydrolysis lipids rhodamine b Environmental technology. Sanitary engineering TD1-1066
spellingShingle	biological wastewater treatment enzyme activity hydrolysis lipids rhodamine b Environmental technology. Sanitary engineering TD1-1066 P. Van Gaelen D. Springael I. Smets Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
description	Lipids are one of the main organic components in industrial and municipal wastewaters. Lipid hydrolysis is the first step in the biological conversion process and requires a close contact between lipid emulsion droplets and microbial hydrolytic enzymes. Adequate lipid hydrolysis monitoring is crucial to obtain mechanistic knowledge on lipid hydrolysis in response to changes in the process conditions and to improve the overall lipid conversion efficiency in aerobic and anaerobic wastewater treatment systems. We set out to develop a high-throughput lipid hydrolysis monitoring method based on vegetable oil model substrates and fluorescent quantification of product formation by exploiting the interaction with Rhodamine B. Olive oil and soybean oil emulsions were prepared with a high interfacial area and acceptable emulsion stability. The method was easy to apply and allowed to obtain detailed kinetic data over a time course of several hours for up to 16 samples in parallel. A proof-of-concept was obtained with a commercial enzyme, Amano lipase, but remains to be provided for wastewater treatment sludge samples. The findings of this study pave the way for further method development in lipid hydrolysis monitoring. Highlights Lipid hydrolysis monitoring is essential to optimize (an)aerobic wastewater treatment.; High-throughput monitoring methods are required.; The established p-nitrophenol palmitate method is not adequate.; A lipid assay model substrate should resemble the actual lipids present in wastewater.; Fluorescent lipid hydrolysis quantification with Rhodamine B is a promising approach for high-throughput monitoring.;
format	article
author	P. Van Gaelen D. Springael I. Smets
author_facet	P. Van Gaelen D. Springael I. Smets
author_sort	P. Van Gaelen
title	Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
title_short	Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
title_full	Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
title_fullStr	Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
title_full_unstemmed	Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
title_sort	lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/0ed688746bd44beb8b551fdba7d552fe
work_keys_str_mv	AT pvangaelen lipidhydrolysismonitoringinwastewatertreatmentproofofconceptforahighthroughputvegetableoilemulsionbasedassay AT dspringael lipidhydrolysismonitoringinwastewatertreatmentproofofconceptforahighthroughputvegetableoilemulsionbasedassay AT ismets lipidhydrolysismonitoringinwastewatertreatmentproofofconceptforahighthroughputvegetableoilemulsionbasedassay
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Lipid hydrolysis monitoring in wastewater treatment: proof-of-concept for a high throughput vegetable oil emulsion based assay

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