A new experimental model for the investigation of sequential hermaphroditism

Abstract The stunning sexual transformation commonly triggered by age, size or social context in some fishes is one of the best examples of phenotypic plasticity thus far described. To date our understanding of this process is dominated by studies on a handful of subtropical and tropical teleosts, o...

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Autores principales:	A. Goikoetxea, S. Muncaster, E. V. Todd, P. M. Lokman, H. A. Robertson, C. E. De Farias e Moraes, E. L. Damsteegt, N. J. Gemmell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ed7e1382f6d4f9e8a6c1602ce62481f
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spelling	oai:doaj.org-article:0ed7e1382f6d4f9e8a6c1602ce62481f2021-11-28T12:17:21ZA new experimental model for the investigation of sequential hermaphroditism10.1038/s41598-021-02063-y2045-2322https://doaj.org/article/0ed7e1382f6d4f9e8a6c1602ce62481f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02063-yhttps://doaj.org/toc/2045-2322Abstract The stunning sexual transformation commonly triggered by age, size or social context in some fishes is one of the best examples of phenotypic plasticity thus far described. To date our understanding of this process is dominated by studies on a handful of subtropical and tropical teleosts, often in wild settings. Here we have established the protogynous New Zealand spotty wrasse, Notolabrus celidotus, as a temperate model for the experimental investigation of sex change. Captive fish were induced to change sex using aromatase inhibition or manipulation of social groups. Complete female-to-male transition occurred over 60 days in both cases and time-series sampling was used to quantify changes in hormone production, gene expression and gonadal cellular anatomy. Early-stage decreases in plasma 17β-estradiol (E2) concentrations or gonadal aromatase (cyp19a1a) expression were not detected in spotty wrasse, despite these being commonly associated with the onset of sex change in subtropical and tropical protogynous (female-to-male) hermaphrodites. In contrast, expression of the masculinising factor amh (anti-Müllerian hormone) increased during early sex change, implying a potential role as a proximate trigger for masculinisation. Collectively, these data provide a foundation for the spotty wrasse as a temperate teleost model to study sex change and cell fate in vertebrates.A. GoikoetxeaS. MuncasterE. V. ToddP. M. LokmanH. A. RobertsonC. E. De Farias e MoraesE. L. DamsteegtN. J. GemmellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Goikoetxea S. Muncaster E. V. Todd P. M. Lokman H. A. Robertson C. E. De Farias e Moraes E. L. Damsteegt N. J. Gemmell A new experimental model for the investigation of sequential hermaphroditism
description	Abstract The stunning sexual transformation commonly triggered by age, size or social context in some fishes is one of the best examples of phenotypic plasticity thus far described. To date our understanding of this process is dominated by studies on a handful of subtropical and tropical teleosts, often in wild settings. Here we have established the protogynous New Zealand spotty wrasse, Notolabrus celidotus, as a temperate model for the experimental investigation of sex change. Captive fish were induced to change sex using aromatase inhibition or manipulation of social groups. Complete female-to-male transition occurred over 60 days in both cases and time-series sampling was used to quantify changes in hormone production, gene expression and gonadal cellular anatomy. Early-stage decreases in plasma 17β-estradiol (E2) concentrations or gonadal aromatase (cyp19a1a) expression were not detected in spotty wrasse, despite these being commonly associated with the onset of sex change in subtropical and tropical protogynous (female-to-male) hermaphrodites. In contrast, expression of the masculinising factor amh (anti-Müllerian hormone) increased during early sex change, implying a potential role as a proximate trigger for masculinisation. Collectively, these data provide a foundation for the spotty wrasse as a temperate teleost model to study sex change and cell fate in vertebrates.
format	article
author	A. Goikoetxea S. Muncaster E. V. Todd P. M. Lokman H. A. Robertson C. E. De Farias e Moraes E. L. Damsteegt N. J. Gemmell
author_facet	A. Goikoetxea S. Muncaster E. V. Todd P. M. Lokman H. A. Robertson C. E. De Farias e Moraes E. L. Damsteegt N. J. Gemmell
author_sort	A. Goikoetxea
title	A new experimental model for the investigation of sequential hermaphroditism
title_short	A new experimental model for the investigation of sequential hermaphroditism
title_full	A new experimental model for the investigation of sequential hermaphroditism
title_fullStr	A new experimental model for the investigation of sequential hermaphroditism
title_full_unstemmed	A new experimental model for the investigation of sequential hermaphroditism
title_sort	new experimental model for the investigation of sequential hermaphroditism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ed7e1382f6d4f9e8a6c1602ce62481f
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A new experimental model for the investigation of sequential hermaphroditism

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