Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice

Abstract Since it was first characterised in 1983, 2-acetyl-1-pyrroline (2AP) has been considered to be the most important aroma compound in rice. In this study, we show four other amine heterocycles: 6-methyl, 5-oxo-2,3,4,5-tetrahydropyridine (6M5OTP), 2-acetylpyrrole, pyrrole and 1-pyrroline, that...

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Autores principales:	Venea Dara Daygon, Mariafe Calingacion, Louise C. Forster, James J. De Voss, Brett D. Schwartz, Ben Ovenden, David E. Alonso, Susan R. McCouch, Mary J. Garson, Melissa A. Fitzgerald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ee4361e154b4b67b3ea808a8f8ad96a
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spelling	oai:doaj.org-article:0ee4361e154b4b67b3ea808a8f8ad96a2021-12-02T15:05:43ZMetabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice10.1038/s41598-017-07693-92045-2322https://doaj.org/article/0ee4361e154b4b67b3ea808a8f8ad96a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07693-9https://doaj.org/toc/2045-2322Abstract Since it was first characterised in 1983, 2-acetyl-1-pyrroline (2AP) has been considered to be the most important aroma compound in rice. In this study, we show four other amine heterocycles: 6-methyl, 5-oxo-2,3,4,5-tetrahydropyridine (6M5OTP), 2-acetylpyrrole, pyrrole and 1-pyrroline, that correlate strongly with the production of 2AP, and are present in consistent proportions in a set of elite aromatic rice varieties from South East Asia and Australia as well as in a collection of recombinant inbred lines (RILs) derived from indica Jasmine-type varieties, Australian long grain varieties (temperate japonica) and Basmati-type rice (Grp V). These compounds were detected through untargeted metabolite profiling by two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF-MS), and their identity were confirmed by comparison with authentic standards analysed using gas chromatography mass spectrometry (GC-MS) and High Resolution GC × GC-TOF-MS (GC × GC HRT-4D). Genome-wide association analysis indicates that all compounds co-localised with a single quantitative trait locus (QTL) that harbours the FGR gene responsible for the production of GABA. Together, these data provide new insights into the production of 2AP, and evidence for understanding the pathway leading to the accumulation of aroma in fragrant rice.Venea Dara DaygonMariafe CalingacionLouise C. ForsterJames J. De VossBrett D. SchwartzBen OvendenDavid E. AlonsoSusan R. McCouchMary J. GarsonMelissa A. FitzgeraldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Venea Dara Daygon Mariafe Calingacion Louise C. Forster James J. De Voss Brett D. Schwartz Ben Ovenden David E. Alonso Susan R. McCouch Mary J. Garson Melissa A. Fitzgerald Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
description	Abstract Since it was first characterised in 1983, 2-acetyl-1-pyrroline (2AP) has been considered to be the most important aroma compound in rice. In this study, we show four other amine heterocycles: 6-methyl, 5-oxo-2,3,4,5-tetrahydropyridine (6M5OTP), 2-acetylpyrrole, pyrrole and 1-pyrroline, that correlate strongly with the production of 2AP, and are present in consistent proportions in a set of elite aromatic rice varieties from South East Asia and Australia as well as in a collection of recombinant inbred lines (RILs) derived from indica Jasmine-type varieties, Australian long grain varieties (temperate japonica) and Basmati-type rice (Grp V). These compounds were detected through untargeted metabolite profiling by two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF-MS), and their identity were confirmed by comparison with authentic standards analysed using gas chromatography mass spectrometry (GC-MS) and High Resolution GC × GC-TOF-MS (GC × GC HRT-4D). Genome-wide association analysis indicates that all compounds co-localised with a single quantitative trait locus (QTL) that harbours the FGR gene responsible for the production of GABA. Together, these data provide new insights into the production of 2AP, and evidence for understanding the pathway leading to the accumulation of aroma in fragrant rice.
format	article
author	Venea Dara Daygon Mariafe Calingacion Louise C. Forster James J. De Voss Brett D. Schwartz Ben Ovenden David E. Alonso Susan R. McCouch Mary J. Garson Melissa A. Fitzgerald
author_facet	Venea Dara Daygon Mariafe Calingacion Louise C. Forster James J. De Voss Brett D. Schwartz Ben Ovenden David E. Alonso Susan R. McCouch Mary J. Garson Melissa A. Fitzgerald
author_sort	Venea Dara Daygon
title	Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
title_short	Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
title_full	Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
title_fullStr	Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
title_full_unstemmed	Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
title_sort	metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0ee4361e154b4b67b3ea808a8f8ad96a
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Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice

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