Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering

Promising strategies for neural tissue engineering are based on the use of three-dimensional substrates for cell anchorage and tissue development. In this work, fibrillar scaffolds composed of electrospun randomly- and aligned-oriented fibers coated with plasma synthesized pyrrole polymer, doped and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Diana María Osorio-Londoño, José Rafael Godínez-Fernández, Ma. Cristina Acosta-García, Juan Morales-Corona, Roberto Olayo-González, Axayácatl Morales-Guadarrama
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plasma polymerization pyrrole electrospun scaffolds 3D cell culture Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/0ee805e15e9544c7aa6a56924f780cfa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0ee805e15e9544c7aa6a56924f780cfa
record_format	dspace
spelling	oai:doaj.org-article:0ee805e15e9544c7aa6a56924f780cfa2021-11-25T18:47:51ZPyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering10.3390/polym132238762073-4360https://doaj.org/article/0ee805e15e9544c7aa6a56924f780cfa2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3876https://doaj.org/toc/2073-4360Promising strategies for neural tissue engineering are based on the use of three-dimensional substrates for cell anchorage and tissue development. In this work, fibrillar scaffolds composed of electrospun randomly- and aligned-oriented fibers coated with plasma synthesized pyrrole polymer, doped and undoped with iodine, were fabricated and characterized. Infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction analysis revealed the functional groups and molecular integration of each scaffold, as well as the effect of plasma polymer synthesis on crystallinity. Scanning microscopy imaging demonstrated the porous fibrillar micrometric structure of the scaffolds, which afforded adhesion, infiltration, and survival for the neural cells. Orientation analysis of electron microscope images confirmed the elongation of neurite-like cell structures elicited by undoped plasma pyrrole polymer-coated aligned scaffolds, without any biochemical stimuli. The MTT colorimetric assay validated the biocompatibility of the fabricated composite materials, and further evidenced plasma pyrrole polymer-coated aligned scaffolds as permissive substrates for the support of neural cells. These results suggest plasma synthesized pyrrole polymer-coated aligned scaffolds are promising materials for tissue engineering applications.Diana María Osorio-LondoñoJosé Rafael Godínez-FernándezMa. Cristina Acosta-GarcíaJuan Morales-CoronaRoberto Olayo-GonzálezAxayácatl Morales-GuadarramaMDPI AGarticleplasma polymerizationpyrroleelectrospun scaffolds3D cell cultureOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3876, p 3876 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plasma polymerization pyrrole electrospun scaffolds 3D cell culture Organic chemistry QD241-441
spellingShingle	plasma polymerization pyrrole electrospun scaffolds 3D cell culture Organic chemistry QD241-441 Diana María Osorio-Londoño José Rafael Godínez-Fernández Ma. Cristina Acosta-García Juan Morales-Corona Roberto Olayo-González Axayácatl Morales-Guadarrama Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
description	Promising strategies for neural tissue engineering are based on the use of three-dimensional substrates for cell anchorage and tissue development. In this work, fibrillar scaffolds composed of electrospun randomly- and aligned-oriented fibers coated with plasma synthesized pyrrole polymer, doped and undoped with iodine, were fabricated and characterized. Infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction analysis revealed the functional groups and molecular integration of each scaffold, as well as the effect of plasma polymer synthesis on crystallinity. Scanning microscopy imaging demonstrated the porous fibrillar micrometric structure of the scaffolds, which afforded adhesion, infiltration, and survival for the neural cells. Orientation analysis of electron microscope images confirmed the elongation of neurite-like cell structures elicited by undoped plasma pyrrole polymer-coated aligned scaffolds, without any biochemical stimuli. The MTT colorimetric assay validated the biocompatibility of the fabricated composite materials, and further evidenced plasma pyrrole polymer-coated aligned scaffolds as permissive substrates for the support of neural cells. These results suggest plasma synthesized pyrrole polymer-coated aligned scaffolds are promising materials for tissue engineering applications.
format	article
author	Diana María Osorio-Londoño José Rafael Godínez-Fernández Ma. Cristina Acosta-García Juan Morales-Corona Roberto Olayo-González Axayácatl Morales-Guadarrama
author_facet	Diana María Osorio-Londoño José Rafael Godínez-Fernández Ma. Cristina Acosta-García Juan Morales-Corona Roberto Olayo-González Axayácatl Morales-Guadarrama
author_sort	Diana María Osorio-Londoño
title	Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
title_short	Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
title_full	Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
title_fullStr	Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
title_full_unstemmed	Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
title_sort	pyrrole plasma polymer-coated electrospun scaffolds for neural tissue engineering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0ee805e15e9544c7aa6a56924f780cfa
work_keys_str_mv	AT dianamariaosoriolondono pyrroleplasmapolymercoatedelectrospunscaffoldsforneuraltissueengineering AT joserafaelgodinezfernandez pyrroleplasmapolymercoatedelectrospunscaffoldsforneuraltissueengineering AT macristinaacostagarcia pyrroleplasmapolymercoatedelectrospunscaffoldsforneuraltissueengineering AT juanmoralescorona pyrroleplasmapolymercoatedelectrospunscaffoldsforneuraltissueengineering AT robertoolayogonzalez pyrroleplasmapolymercoatedelectrospunscaffoldsforneuraltissueengineering AT axayacatlmoralesguadarrama pyrroleplasmapolymercoatedelectrospunscaffoldsforneuraltissueengineering
_version_	1718410693580947456

Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering

Ejemplares similares